Proceedings of the National Academy of Sciences
Articles in this Issue
L-lactic acid enhances type I interferon immune response and inhibits virus infection by promoting IRF9 L-lactylation
Lysine lactylation is a crucial posttranslational modification (PTM) that regulates protein function. Here, this study revealed that L-lactic acid promotes host immune response and inhibits viral infe...
Dosa: A method to covalently barcode proteins for high-throughput biochemistry
Deep mutational scanning couples a protein’s activity to DNA sequencing for high-throughput assessment of the effects of all single amino acid substitutions, but it largely uses indirect assays, like...
Shouldering the challenge of deciphering avian palate evolution
Tetraspanin TSP-12 and SUP-17/ADAM10 exhibit cell type–specific codependence for trafficking through the Golgi
A subset of the four-pass transmembrane proteins called C8 tetraspanins (TspanC8) can bind and promote the cell surface localization of ADAM10 (A Disintegrin and Metalloproteinase 10). ADAM10 is a con...
Cotranslational assembly directs the biogenesis of the m <sup>6</sup> A methyltransferase complex
The chemical modification N 6 -methyladenosine (m 6 A) is catalyzed by the m 6...
Polymer conformational entropy as the driver of complex coacervation
Understanding the mechanisms leading to complex coacervation is a fundamental challenge in biomolecular sciences. Here, we explored the condensation transition and pretransitional behavior of an arche...
Combinatorial treatment of glioblastoma with temozolomide (TMZ) plus 5-ethynyl-2′-deoxyuridine (EdU)
Glioblastoma (GBM) is the most aggressive malignant primary brain tumor in adults, with incidence peaking in later life. It is commonly treated with surgery followed by administration of ionizing radi...
Controlled dynamic remodeling of the spliceosome active site enables the first step of splicing
Recent cryoelectron microscopy data have revealed significant conformational rearrangements of the megadalton spliceosome structure during splicing, an essential catalytic process for maturing most hu...
Correction for Xiao et al., The modern pattern of insect herbivory predates the advent of angiosperms by 60 My
Life without sex: Lack of desire or traumatic retire?
Glycosylation of GETV E2 promotes pathogenesis in animal models
Getah virus (GETV) is a mosquito-borne alphavirus. It has posed a threat to various livestock and humans during decades of evolution and spread. However, the viral determinants and mechanisms underlyi...
Locating a control of plant and ecosystem water fluxes deep in the leaf
MET goes to the membrane—A new place for the “old” juvenile hormone receptor
Competition between a transmembrane helix on Scap and a membrane cholesterol regulates Scap–Insig interaction and SREBP activation
Cholesterol homeostasis in mammalian cells relies on the interaction between two endoplasmic reticulum (ER) sterol-sensing membrane proteins, Scap and Insig. Their interaction regulates activation of...
Peptide-based covalent inhibitor of tubulin detyrosination promotes mesenchymal-to-epithelial transition in lung cancer cells
Detyrosination is a reversible posttranslational modification specific to α-tubulin, which has been implicated in cancer progression and invasiveness by promoting epithelial-to-mesenchymal transition....
Mass spectrometry footprinting reveals how kinetic stabilizers counteract transthyretin dynamics altered by pathogenic mutations
The aggregation of transthyretin (TTR) results in life-threatening transthyretin amyloidosis. Familial forms of the disease arise from point mutations that destabilize the TTR tetramer, leading to its...
How attention-like behavior emerges in an artificial neural network
Competition for heritable wealth, not cultural group selection, drives the evolution of monogamy
The decline of polygyny and the rise of monogamy among complex, stratified societies characterized by high wealth inequality present a long-standing puzzle in anthropology. Competing explanations sugg...
Systematic identification of microtubule lumen proteins reveals a taxane-sensitive luminal resident JPT2 regulating MEC17 accessibility
Microtubules have been extensively studied for their interactions at the outer surface and ends, yet the regulatory mechanisms within the microtubule lumen, particularly in singlet microtubules, remai...
Dual-state stepwise methane-to-methanol conversion by water droplets with excellent yield and selectivity
Leveraging the continuous and rapid deposition and evaporation processes of water microdroplets on photocatalyst surfaces, this study presents an innovative dual-state stepwise methane conversion appr...
Experimental manipulation of ecological and cognitive conditions produces the entire conformity–diversity spectrum in a single species
Identifying the factors that drive individuals in a population to conform to the same behavior or to exhibit behavioral diversity is crucial for understanding collective decision-making, information t...
Frustrated random-singlet state with ice-type structural fluctuation in spinel titanates
In ice, it is well known that the orientation of H 2 O molecules is disordered by geometrical frustration. Ice-analogous materials having a pyrochlore lattice d...
Intrinsic OASL expression governs heterogeneity in interferon induction during influenza A virus infection
Effective control of viral infection requires rapid induction of the innate immune response, especially the type I and type III interferon (IFN) systems. Despite the critical role of IFN induction in...
Circular RNA–based therapy targeting metabolic vulnerability of fatty acid synthesis overcomes castration-resistant prostate cancer
Androgen receptor (AR) signaling is essential for prostate cancer (PCa) cell growth and remains a key therapeutic target in castration-resistant PCa (CRPC). While circular RNAs (circRNAs) are increasi...
NFE2L1/Nrf1 forms a coactivator complex post-peptide: <i>N</i> -glycanase-mediated sequence editing and mitigates proteasome dysfunction
Nuclear factor erythroid 2-like 1 (NFE2L1/Nrf1), an endoplasmic reticulum (ER)-associated transcription factor, is responsible for the coordinated expression of proteasome subunit genes upon proteasom...