Proceedings of the National Academy of Sciences
Articles in this Issue
Deep learning reveals how cells pull, buckle, and navigate fibrous environments
Cells in tissues navigate fibrous environments fundamentally differently than they do on flat substrates, but the establishment of cell forces in physiological fibrous settings remains poorly understo...
Artificial cells with liquid–liquid phase separation–regulated cell-free protein synthesis
The rapid advancement of synthetic biology has enabled the construction of artificial cells that closely mimic the morphology and functionality of their natural counterparts. However, significant limi...
Emergence of bulk-like structural features and 2D-to-3D transition in boron nanoclusters
As an electron-deficient element, boron possesses fascinating three-dimensional structures and unconventional chemical bonds. Nanoclusters of boron have also been found to exhibit intriguing structura...
The telomeric valine–arginine dipeptide repeat protein changes state to diffuse staining in mitosis and represses in vitro translation
Translation of mammalian G-rich telomeric RNA via the Repeat Associated non-AUG (AUG, the mRNA start codon) mechanism can produce proteins consisting of long repeats of valine–arginine (VR) or glycine...
Interactions between long- and short-term synaptic plasticity transform temporal neural representations into spatial
Information processing in the brain relies on the transmission of spikes through chemical synapses whose efficacies often depend on their recent firing history. While effects of such short-term plasti...
Breast cancer cell coculture induces normal lung fibroblast transition to CAFs, promoting tumor cell dormancy and therapy resistance
Cancer-associated fibroblasts (CAFs) shape the tumor microenvironment of primary breast tumors to promote tumor progression and therapy resistance. While the lung is a top metastatic site in breast ca...
Longitudinal transformation of mitochondrial metabolism during neurogenesis
Neural stem cells (NSCs) are valuable in the quest to conquer neurodegenerative diseases due to their capability to reconstruct the damaged neuronal networks. However, deep understanding of the interc...
Cloud fraction response to aerosol driven by nighttime processes
Aerosol–cloud interactions remain one of the largest uncertainties in the anthropogenic forcing of the climate; a significant contribution to this is due to the aerosol effect on the development of cl...
Correction for Robinson et al., Dried fish provide widespread access to critical nutrients across Africa
Targeting orthotopic and metastatic pancreatic cancer with allogeneic stem cell–engineered mesothelin-redirected CAR-NKT cells
Pancreatic cancer (PC) remains one of the leading causes of cancer-related mortality worldwide. The majority of patients are diagnosed at advanced stages, with over 50% presenting with metastatic dise...
Perception of own centrality in social networks
This study explores how individuals perceive their social networks, with a focus on their own positioning. Using experimental methods and network analysis, we show that people have a limited understan...
Dynamics and variegation in the Treg response to Interleukin-2
Interleukin-2 (IL2) is the key trophic factor for T regulatory (Treg) cells, controlling their differentiation and homeostasis. To understand how temporally regulated responses to IL2 unfold in Tregs,...
Slow relaxation and landscape-driven dynamics in viscous ripening foams
Foams and dense emulsions display complex mechanical behavior, including intermittent rearrangement dynamics, power-law rheology, and slow recovery after perturbation. These effects have long been con...
Static morphogen scaling enables proportional growth in a tissue growth model inspired by axolotl limb regeneration
Axolotls can regenerate lost limbs throughout life, while they continue to grow. This poses the question of how the size and pattern of a regenerating limb is matched to a widely varying animal size....
Turbulence in the terrestrial magnetosheath: Space–time correlation using the Magnetospheric Multiscale mission
Spatiotemporal correlation of magnetic field fluctuations is investigated using the Magnetospheric Multiscale mission in the terrestrial magnetosheath. The first observation of the turbulence propagat...
High-throughput screening for class I peptide MHC binding via yeast surface display
T cells rely on short peptides presented by highly polymorphic major histocompatibility complexes (MHCs) to selectively initiate adaptive immune responses. Despite its importance, few techniques can s...
Ribonuclease RNase Z is an evolutionarily conserved deAMPylase
Protein AMPylation is a highly conserved posttranslational modification in which adenosine monophosphate (AMP) is covalently attached to protein substrates. Our studies revealed that the mitochondrial...
The potential existential threat of large language models to online survey research
The advancement of large language models poses a severe, potentially existential threat to online survey research, a fundamental tool for data collection across the sciences. This work demonstrates th...
Engineering a spatiotemporal macrophage circuit via STING phase separation to override immune suppression in pancreatic cancer
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies, largely due to its highly immunosuppressive tumor microenvironment (TME), which fuels metastasis and resistance to...
Putative muscle stem cells promote <i>Xenopus</i> tail regeneration by modifying macrophage function via <i>c1qtnf3</i>
In Xenopus laevis tadpole tail regeneration, lineage-restricted tissue stem cells produce differentiated cells that...
The immunoproteasome regulates ILC2 responses by modulating mitochondrial capacity
Type 2 innate lymphoid cells (ILC2s) contribute to type 2 immunity but have also been associated with multiple inflammatory diseases, including airway inflammation and asthma. We report that beyond it...
Autism-associated <i>Scn2a</i> haploinsufficiency disrupts in vivo dendritic signaling and impairs flexible decision-making
SCN2A is a high-confidence risk gene for autism spectrum disorder. Loss-of-function mutations in Scn2a reduce dendritic excitability in neoc...
OPEN STOMATA 1 activates SLAC1 anion channel primarily through CPK15 in ABA-induced stomatal closure in <i>Arabidopsis</i>
The Ca 2+ -independent OST1 and Ca 2+ -dependent protein kinases CPKs both activate the anion channel SLAC1 during ABA-i...
Capped high-force integrin bond lifetimes and spacing-tuned binding frequency drive rapid fibroblast migration
Cell migration relies on balancing focal adhesion (FA) stability—necessary for traction generation—and turnover—essential for forward translocation. Here, we dissect how integrin binding frequency and...
Genome integrity relies on rapid recycling of DNA Pol III in bacteria
DNA replication requires precise coordination between DNA unwinding and DNA synthesis. In all domains of life, protein–protein interactions at the replisome maintain proximity between the enzymes that...