PLoS ONE
Articles in this Issue
Integrated metabolomics and bioactivity analysis of new chrysanthemum cultivar petals: Insights into eye-protecting agents
Chrysanthemum is a globally significant economical crop. Through broadly targeted metabolomics, 58 polyphenols and 65 xanthophylls were quantitatively and qualitatively assessed in chrysanthemum petal...
Cardiac markers in children and adolescents at the onset of type 1 diabetes mellitus
Background The incidence of type 1 diabetes mellitus (T1DM) in children and adolescents is rising, and both micro- and macrovascular complications pose significant health risks. Fo...
Feasibility and testing outcomes of task-shared implementation of advanced HIV disease point of care tests in Beira (Mozambique) and Kinshasa (DRC)
Introduction Mortality from advanced HIV disease (AHD) remains high and current strategies to promptly test people eligible for AHD screening, are insufficient. Task sharing for po...
Individual and interaction effects of health determinants on health-related quality of life in Korean adults aged 50–81 years: A causal Bayesian network analysis
Health-related quality of life (HRQoL) reflects physical and mental well-being and is increasingly important in aging populations, yet traditional approaches often fail to capture the complex causal p...
Genome-wide identification and characterization of argonaute, dicer-like, and RNA-dependent RNA polymerase gene families in potato (Solanum tuberosum): Advancing RNA interference-based crop enhancement
In eukaryotic species, RNA silencing is a conserved mechanism for controlling gene expression. The RNA-dependent RNA polymerase (RDR), argonaute (AGO) , and...
High accuracy breast cancer classification with BIRADS and coclustering
Breast cancer is one of the most common disease in women. Most of existing breast cancer classification methods include region segmentation, feature extraction and classification phases. It is hard fo...
Building an implementation framework for directly observed feedback by attending physicians
Background Effective formative feedback from attending physicians to residents is critical for competency-based medical education. Feedback curricula commonly focus on simulated fe...
Medial pivot designs result in improved patient reported outcome measures and range of motion when compared to cruciate retaining total knee replacements: A systematic review and meta-analysis
Background Knee arthroplasty remains one of the most important treatment options in improving quality of life for patients with end-stage knee osteoarthritis. However, roughly 20%...
The WEAR-BOT checklist: A risk of bias tool for evaluating validity and reliability research in wearable technology
This paper proposes an innovative tool designed to standardize the evaluation of validity and reliability studies in the rapidly evolving field of wearable technology. We introduce the WEArable Techno...
Broad immunogenicity of house dust mite proteins contrasts restricted specific IgE and IgG4 associated with allergy
Background House dust mite (HDM) allergy involves IgE and T H 2 responses to major and minor allergens. Less is k...
An upstream secondary DNA motif within the IL3 insulator CTCF binding site is required for enhancer-blocking insulator activity
CCCTC-binding Factor (CTCF) is the only known vertebrate protein that functions to organise the genome into distinct functional chromatin domains. A subset of CTCF sites also provide insulator activit...
Development and evaluation of a stepwise clinical competency development program for new nurses: A single-group repeated-measures quasi-experimental study
Background New nurses often encounter adaptation challenges in hospital settings owing to gaps in clinical knowledge and skills, leading to high turnover rates and patient safety c...
The impact of policy restrictions and mobility changes on excess mortality during the COVID-19 pandemic in The Netherlands, 2020–2022
We analyzed the impact of the COVID-19 policy restrictions on mobility patterns and excess mortality at the regional level in The Netherlands between 2020 and 2022. Our analysis combines data on publi...
Regional variations in the diet of the South Polar Skua (Stercorarius maccormicki) in the Ross Sea region, Antarctica
The South Polar Skua ( Stercorarius maccormicki ) is an opportunistic feeder, predator, and scavenger widely distributed in the coastal ecosystems of Antarctica...
Emotion meets coordination: Designing multi-agent LLMs for fine-grained user sentiment detection on social media
Social media platforms have become central channels for emotional communication, posing new challenges for fine-grained sentiment analysis due to their high contextual variability, multimodal content,...
Trends in usage and regional variations in Ethiopian animal feeding practices: A 15-year analysis for informed policy
Livestock feeding practices play a pivotal role in sustaining agricultural productivity and food security in Ethiopia. However, the sector continues to face structural challenges, including a heavy re...
Relationship between land use type and bacterial composition in adjacent streams and riparian zones
Anthropogenic activities can negatively impact riparian and stream ecosystems, resulting in declines in biodiversity and certain ecosystem functions. Microbiomes in these environments play crucial rol...
The 83 symptoms of tinnitus: Content overlap of commonly used scales for tinnitus burden
Background Clinical management of tinnitus remains challenging due to unclear etiology and diverse phenotypic manifestations. To quantify its associated burden, a variety of patien...
Study protocol for a multicenter phase II prospective externally controlled non-inferiority trial of hypofractionated re-irradiation in patients with recurrent high-grade glioma (RISinG)
Background Reirradiation is a widely accepted option for second-line treatment in patients with recurrent glioma. However, no standard radiation regimen has been defined. Hypofract...
Interpretable ensemble machine learning framework for cardiovascular disease prediction using EMR data and large language models in Ethiopia
Cardiovascular diseases (CVDs) are leading causes of morbidity and mortality globally, with a growing burden in low- and middle-income countries such as Ethiopia. Early detection is limited by resourc...
Association between physical activity levels and functional recovery, quality of life, and psychological well-being in patients undergoing physiotherapy for musculoskeletal disorders: A cross-sectional study
Physical activity is known to enhance functional recovery and quality of life (QoL) in individuals with musculoskeletal disorders; however, the extent to which varying levels of physical activity infl...
Impact of protected areas on deforestation in Madagascar from 2000 to 2023: A pre-analysis plan
Protected areas are the most prevalent strategy to mitigate biodiversity loss and deforestation, especially in biodiversity hotspots like Madagascar. This pre-analysis plan outlines the data, methods,...
Exploring physicochemical property and food safety in dried shrimp production: Risks and mitigation strategies
Dried shrimp is a widely consumed aquatic product whose safety and quality depend on various physicochemical and microbiological properties influenced by production practices. This study aimed to asse...
Professional dancers’ beliefs and conceptualisations of their posture and movement: A qualitative research study
Many issues are unresolved regarding posture and movement and their utility as target for rehabilitation and function. As movement experts, elite ballet and contemporary dancers could provide insights...
Opportunities and challenges of implementing an urban primary healthcare delivery model: Programmatic lessons from Aalo Clinic, Bangladesh
Background Rapid urbanization in Bangladesh has strained the healthcare system, leaving urbanites underserved in primary healthcare (PHC). To address this gap, a UNICEF-supported G...