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Warming Alaskan rivers affect first-year growth in critical northern food fishes

Ammonia Evolution in Glycine Pyrolysis via Ionic-Pair Reaction Mechanisms

A hybrid quantitative approach for assessment of geotechnical hazards in rock tunnels using finite element and variation coefficient methods

Nickel-Catalyzed Enantioconvergent Cross-Electrophile Coupling of Benzylic Alcohols with Alkenyl Electrophiles

Structural Transition from Closed to Open for the Influenza A M2 Proton Channel as Observed by Proton-Detected Solid-State NMR

Assessment of the in vitro antimicrobial activity and fatty acid composition of crocodile oil from Crocodylus siamensis

<i><b>N</b></i>-Oxide-Driven Heme-Activatable Biomolecule Labeling for Visualization of Labile Heme in Living Cells and Mouse Brain

Incidence and risk factors of new-onset hypertension up to 3 years post SARS-CoV-2 infection

Unveiling Water-Vapor-Promoted Oxidation of Palladium Nanoparticles via Atomic-Scale Transmission Electron Microscopy at Atmospheric Pressure

Metabolically engineered plant cell cultures as biofactories for the production of high-value carotenoids astaxanthin and canthaxanthin

Abstract Astaxanthin and canthaxanthin are high-value carotenoids with growing demand due to their antioxidant properties and applications in food, cosmetic, and pharmaceutical sectors. However, natural sources are limited and current production methods are often costly or unsustainable. In this study, we developed a plant-based platform for ketocarotenoid biosynthesis using metabolically engineered Nicotiana tabacum BY-2 cell suspension cultures. Specifically, we expressed a marine bacterial crtW gene ( β-carotene ketolase ) alone or in combination with overexpressed plant psy ( phytoene synthase ) and crtI ( phytoene desaturase ) genes. The resulting cell lines displayed visually distinct pigmentation and accumulated different ketocarotenoid profiles based on their genetic modifications. Single-gene transformants expressing crtW produced up to 50 µg g⁻¹ DW of canthaxanthin and 127 µg g⁻¹ DW of astaxanthin. Co-expression of all three genes significantly increased canthaxanthin accumulation to 788 µg g⁻¹ DW. Our results establish suspended undifferentiated plant cells as a scalable and sustainable system for ketocarotenoid production, offering a biological alternative to natural producers and chemical synthesis.

Electronic Modification of a Reduced Mononuclear Nonheme Iron Nitrosyl Complex Leads to HNO Release

Novel SMA BASED Elmanspiking neural network modelled fuzzy PI controller for speed-torque regulation of PMSM

O-Induced Diatomic Fe–Mo Mimetic Enzyme for Efficient Electrocatalytic Nitrogen Reduction at Universal pH

Interplay of electronic and magnetic phase modulation in a spin-polarised nanomagnet

Daily briefing: Reflections from a survivor of the Hiroshima bombing

Predictions of Steady-State Photo-CIDNP Enhancement by Machine Learning

Investigation of parallel joint density thresholds for granite tunnel failure based on physical model experiments and multiscale monitoring techniques

Isolation of Diamond Spin Chains in a Layered Halide Perovskite Heterostructure

Natural history study of recessive dystrophic epidermolysis bullosa wounds and patient reported outcomes using mobile application home photography

Symmetric Boron-Bridged Carbon Quantum Frameworks for Light-Emitting Diodes with over 20% External Quantum Efficiency