Browse Articles
Discover research articles across all indexed journals
Structural characterization of intermediates in Sec1/Munc18 protein-catalyzed SNARE assembly
Construction of Isolated Pd <sub>3</sub> Geometry on GaO <sub> <i>x</i> </sub> -Modified Pd/Al <sub>2</sub> O <sub>3</sub> as a Highly Active and Selective Catalyst for Semihydrogenation of Acetylene
The application of contrast-enhanced T2-FLAIR MRI in gross tumor target volume determination for radiotherapy of large-volume brain metastases
Informing HIV vaccine design
Alternative Extracellular Disulfide Bond Formation by N-terminal Cysteines Supports Functional Expression of Odorant Receptors
A Water-Soluble Diarylethene Base for Light-Controlled pH Modulation in Biological Systems
Efficacy of historical context and exogenous features on deep learning for cooling load forecasting in chilled water plants
Inhibiting lactate transport reduces lung fibrosis
p53 regulates mitochondrial function and alpha-synuclein aggregation in Parkinson's disease
Targeting rapidly cycling receptors CD2 and CD7 increases nanoparticle delivery to primary CD4+ T cells
Abstract T cells are critically important to many diseases but are traditionally difficult to transfect. We hypothesise that the delivery of therapeutic cargo to T cells can be improved by targeting nanoparticles to surface receptors that undergo rapid receptor-mediated endocytosis. Using an internalisation assay that labelled intracellular and surface proteins with different fluorophores, we find that CD2 and CD7 exhibit significantly higher internalisation than other T cell receptors, such as CD3 or CD4. Targeting CD2 and CD7 improves nanoparticle internalisation by non-stimulated, primary CD4 + T cells and enhances the specificity of association to CD4 + T cells. Similarly, functionalising mRNA-lipid nanoparticles with antibodies targeting CD2 or CD7 enhances mRNA delivery to CD4 + T cells in vitro. Importantly, targeting CD2 or CD7 enables efficient lipid nanoparticle-mediated delivery of mRNA to T cells in blood and lymphoid tissue in vivo, demonstrating that targeting T cell receptor endocytosis can enhance nanoparticle-mediated drug delivery to T cells.
Mass spectrometry-based proteomics to study gastric carcinogenesis: pathophysiological molecular characterization
Inhibiting Nipah virus
Molecular and Structural Dissection of Psalmopoeus pulcher Venom Reveals Cysteine-Rich Peptides that Target Acid-Sensing Ion Channel 1a
Ultralong, spin-photon fibres enable polarization-enhanced wearable sensing
Late-Stage Carboranylation of Nucleos(t)ides Enabled by Minisci and Pd-Catalyzed B–N Cross-Coupling Reactions: Toward the Synthesis of Carboranyl-Modified Oligonucleotides
Bio-efficacy of Syzygium aromaticum bud extract as eco-friendly green acaricide for controlling Hyalomma dromedarii
Abstract The most common hard tick in Egypt is Hyalomma dromedarii as a one of the most dangerous ectoparasites. The current study aims to detect the chemical composition of Syzygium aromaticum bud ethanolic extract using GC-MS and some phytochemical analysis in addition to investigating its acaricidal activity on H . dromedarii engorged females through biological and biochemical studies. The GC-MS revealed the presence of eugenol, eugenol acetate and D-(-)-fructofuranose as the most active compounds, and it appeared to be rich in alkaloids, flavonoids, phenolic compounds, tannins and terpenoids. At high concentration, the extract caused mortality to reach 100% after 35 days of treatment in engorged females, with LC 50 reached 100.22 and 0.83 mg/mL after the 3rd and 5th week (half and end of examined period) following treatment, respectively. The blood digestion was affected by significantly decreasing in their body weights. Furthermore, the extract showed a decrease in their oviposition, hatchability, and fertility. The extract at 100 mg/mL caused a reduction in ovary total protein content with disappearance of some protein bands using SDS-PAGE, compared with the control group. Overall, the current study revealed the effectiveness of S . aromaticum extract as a bio-acaricide for controlling H . dromedarii and it recommended numerous additional field studies to assess its effectiveness under different conditions.