Browse Articles
Discover research articles across all indexed journals
Catalyst-Enabled Chemoselective Metalloradical Activation for Molecular Rearrangement via Ester Migration and Allylic C(sp<sup>3</sup>)–H Amination
Accuracy assessment of a cochlear implant imaging tool using clinical computed tomography images
In Situ Single-Crystal X-ray Diffraction Studies of an Anomalous Nitric Oxide Adsorption in a Partially Activated Metal–Organic Framework
Cefiderocol-resistant pathogens in German hospital wastewater: a reservoir for multidrug resistance
Abstract Cefiderocol-resistant bacteria pose a growing concern in both clinical and environmental settings. This study investigates cefiderocol-resistant bacteria in wastewater from six German tertiary care hospitals. A total of 36 samples were analysed using a culture-dependent approach involving cefiderocol pre-enrichment, yielding 97 cefiderocol-resistant isolates—primarily Enterobacter roggenkampii, Klebsiella oxytoca, Serratia marcescens, and Citrobacter farmeri. Most isolates exhibited high minimum inhibitory concentrations against cefiderocol and resistance to multiple antibiotics. Resistance rates were lower for meropenem-vaborbactam (10.3%) and imipenem-relebactam (33.0%), while all isolates remained susceptible to aztreonam-avibactam. Whole-genome sequencing of 79 isolates revealed a diverse resistome, with 78.5% (62/79) carrying carbapenemase genes. Some isolates harbored up to six distinct β-lactamase genes, including combinations of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and one or multiple carbapenemases—such as as bla OXA−48 co-occurring with bla NDM−1 or bla VIM−1. In addition, biocide and heavy metal resistance genes were prevalent, highlighting bacterial adaptation to harsh environments. Plasmid profiling showed significant interspecies variation, with C. farmeri and K. oxytoca displaying the highest plasmid loads. Across all isolates, 38 unique plasmid incompatibility types were detected, 18 of which were species-specific. These findings highlight the multidrug-resistant nature of wastewater-derived pathogens and the importance of monitoring resistance dissemination in healthcare environments.
Photochemical Insights on Acyl Azolium Salts Enable the Design of a Tandem Hydrogen Atom Transfer/Halogen Atom Transfer Acylation of Alkyl Bromides and Chlorides
Author Correction: EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses
Energy-efficient design of CNTFET-based quaternary arithmetic circuits
Interaction of genetic variants activates latent metabolic pathways in yeast
Abstract Genetic interactions are fundamental to the architecture of complex traits, yet the molecular mechanisms by which variant combinations influence cellular pathways remain poorly understood. Here, we answer the question of whether interactions between genetic variants can activate unique pathways and if such pathways can be targeted to modulate phenotypic outcomes. The model organism Saccharomyces cerevisiae was used to dissect how two causal SNPs, MKT1 89G and TAO3 4477C , interact to modulate metabolic and phenotypic outcomes during sporulation. By integrating time-resolved transcriptomics, absolute proteomics, and targeted metabolomics in isogenic allele replacement yeast strains, we show that the combined presence of these SNPs uniquely activates the arginine biosynthesis pathway and suppresses ribosome biogenesis, reflecting a metabolic trade-off that enhances sporulation efficiency. Functional validation demonstrates that the arginine pathway is essential for mitochondrial activity and efficient sporulation only in the double-SNP background. Our findings show how genetic variant interactions can rewire core metabolic networks, providing a mechanistic framework for understanding polygenic trait regulation and the emergence of additive effects in complex traits.
A novel soft-switched trans-inverse ultra-high-gain DC/DC converter with low switch voltage stress
Reversible Electron Transfer in the Formation of Radical Anionic and Dianionic 1,4-Diborabutatrienes
Gene expression QTL mapping in stimulated iPSC-derived macrophages provides insights into common complex diseases
Abstract Many disease-associated variants are thought to be regulatory but are not present in existing catalogues of expression quantitative trait loci (eQTL). We hypothesise that these variants may regulate expression in specific biological contexts, such as stimulated immune cells. Here, we used human iPSC-derived macrophages to map eQTLs across 24 cellular conditions. We found that 76% of eQTLs detected in at least one stimulated condition were also found in naive cells. The percentage of response eQTLs (reQTLs) varied widely across conditions (3.7% − 28.4%), with reQTLs specific to a single condition being rare (1.11%). Despite their relative rarity, reQTLs were overrepresented among disease-colocalizing eQTLs. We nominated an additional 21.7% of disease effector genes at GWAS loci via colocalization of reQTLs, with 38.6% of these not found in the Genotype–Tissue Expression (GTEx) catalogue. Our study highlights the diversity of genetic effects on expression and demonstrates how condition-specific regulatory variation can enhance our understanding of common disease risk alleles.
Barriers and facilitators for stroke patients’ adherence to rehabilitation in China: a qualitative study based on medical experts
AI versus skin cancer: the future of dermatology diagnosis
Bimolecular Nucleophilic Substitution (S<sub>N</sub>2) Reaction Catalyzed by <scp>l</scp>-Threonine Aldolase
Dyson sphere-like evaporators enhanced interfacial solar evaporation via self-generated internal convection
Thyme essential oil potentials as a bactericidal and biofilm-preventive agent against prevalent bacterial pathogens
Abstract Antimicrobial resistance represents a significant global issue that requires the investigation of innovative approaches for infection management. In pursuit of alternative natural antimicrobials, nine plant essential oils were evaluated for their antibacterial properties against nine common bacterial pathogens. Among the tested essential oils, thyme essential oil demonstrated the highest antibacterial activity against all tested bacterial species, Thyme essential oil exhibited inhibition zones ranging from 17.3 to 51 mm with relative minimum inhibitory concentrations ranging from 99.2 to 450 µg/ml, implying the bactericidal effect. The ultrastructural changes in bacterial cells treated with thyme essential oil were visualized using transmission electron microscope. Thyme essential oil exhibited a potent inhibitory effect toward the biofilm formations for all the tested pathogenic strains. GC/MS analysis was used to determine the thyme essential oil composition. The major components of thyme essential oil were thymol (28.29%), o-cymene (18.31%), ç-terpinene (8.51%), eucalyptol (5%), linalool (2.86%), borneol (2.17%), á-myrcene (1.55%), à-pinene (1.52%) and camphene (1%). Molecular docking analysis demonstrated that the constituents present in the thyme essential oil had high binding affinity for ECF, FimH, LasR, PrfA and RhlA proteins, which were found to be associated with improved anti-biofilm efficacy. Furthermore, treatment with thyme essential oil led to the downregulation of essential genes associated with virulence and biofilm formation in the tested pathogens. These findings suggest that thyme essential oil has promising potential as an antibacterial and a biofilm inhibitory agent to combat bacterial infections in food and pharmaceutical industries.