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The chain mediating role of perceived social support and cognitive reappraisal in the relationship between physical exercise and adolescent social anxiety
MiRNA expression analysis from circulating exosomes in Barrett’s esophagus patients with visceral obesity
Abstract Visceral obesity is a recognized risk factor for Barrett’s esophagus (BE). Circulating exosomal mirnas have been identified as potential biomarkers of BE. The aim of this study was to elucidate the characteristics of plasma exosomal miRNA expression in BE patients with or without visceral obesity and to explore its potential regulatory mechanisms in the pathogenesis of the disease. From June 1, 2017 to August 31, 2020, healthy people and BE patients were recruited at center and divided into four groups: Barrett’s esophagus patients with visceral obesity (VOBE), Barrett’s esophagus patients (BE), visceral obese controls (VOC), and healthy controls (HC).Serum samples were collected after approval by the Ethics Committee, exosomes were isolated by ultrafast centrifugation, and the expression of candidate mirnas was verified by qRT-PCR. Data showed that 19 of the common 27 differential miRNAs were up-regulated and two down-regulated among the three comparison groups of VOBE vs. VOC, VOBE vs. HC and VOBE vs. BE.By integrating TargetScan, miRDB and mirDIP databases, 594 target genes were predicted. The enrichment analysis of KEGG pathway suggested that these genes were significantly enriched in the cancer, PI3K-Akt and other pathway. There is an association between some circulating exosomal miRNAs and Barrett’s esophagus in patients with visceral obesity.This provides some ideas for exploring the molecular mechanism of visceral obesity involved in the development of BE.
Correction: A comprehensive assessment using multiple factors based on HAS-Flow analysis predicts ATL development and progression
Greater exposure to PM2.5 and PM10 is associated with a higher BAD score: the Maastricht study
Differential expression of miR-23a-3p/-210-3p and /-18b-5p in sudden sensorineural hearing loss patients: A North American cohort study
Landslide susceptibility assessment using hybrid geospatial, frequency ratio, and AHP models in Souk Ahras province, Northeastern of Algeria
Steel fibre-reinforced concrete with fully recycled coarse and partially recycled fine aggregates
Combination use of intravenous ketamine-midazolam as a sedative agent in endoscopic retrograde cholangiopancreatography: a randomized control trial
Evaluation of groundwater resources in Wadi Qena, Egypt: a geophysical and hydrogeochemical perspective
Abstract An integrated hydro-geophysical and hydrochemical investigation was conducted to delineate aquifer geometry, assess groundwater potential, and evaluate water quality in the southern part of Wadi Qena, Eastern Desert, Egypt. Eighteen time-domain electromagnetic (TDEM) soundings, ground magnetic profiles, pumping-test data, and six groundwater chemical analyses were jointly interpreted. The integrated datasets reveal five geo-electrical layers and identify two main aquifer systems: a shallow Quaternary aquifer (50–300 m depth; 4.9–86 Ω m) and a deeper Nubian Sandstone aquifer (300–650 m depth; 4.7–17.6 Ω m). Magnetic modeling delineates a variable basement surface (350–850 m) that controls aquifer thickness and the spatial distribution of transmissive zones. Areas of deep basement lows coincide with high-transmissivity wells (655–1170 m 2 /day) and low resistivity, indicating thick, well-connected sandstone bodies. Hydrochemical data (TDS: 1447–1607 mg/L; Na–Cl facies) indicate increasing salinity toward the northwest, consistent with upward leakage along magnetic lineaments and the dissolution of salt-bearing formations. The integrated interpretation demonstrates that combining TDEM, magnetic, and geochemical approaches provides a robust framework for identifying productive aquifers, understanding salinity sources, and optimizing groundwater development in arid terrains.
Identification and functional characterization of splicing factors implicated in mantle cell lymphoma aggressiveness
Machine learning and bioinformatics framework integration reveal potential characteristic genes related to immune cell infiltration in post-traumatic stress disorder
Self-attention enhanced GraphSAGE for UAV fault diagnosis using vibration signals
Temporal changes in Angkorian ironmaking technology in Northern Cambodia during the 10th–14th centuries
Immunomodulatory effects of QsCATH on macrophages: transcriptomic insights and molecular docking analysis
Abstract The molecular mechanisms underlying the immunomodulatory effects of cathelicidins on macrophages remain poorly characterized. This study aimed to elucidate the immunomodulatory mechanisms of QsCATH, an antimicrobial peptide derived from the Chinese spiny frog ( Quasipaa spinosa ), in RAW264.7 macrophages using RNA sequencing and molecular docking. Transcriptomic analysis revealed that QsCATH significantly downregulated inflammatory-related genes ( tnf , il6 , ccl5 , il1b ) and suppressed pro-inflammatory cytokine production (tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β) by inhibiting key pathways such as NF-κB, TNF, and NOD-like receptor signaling. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analyses showed that QsCATH balanced inflammatory resolution with host defense by modulating biological processes including “immune system processes,” “defense responses,” and “oxidative stress responses.” Using molecular docking simulations, potential interactions were predicted between QsCATH and membrane-associated proteins Nod2, Ripk2, and Itga3 (binding scores: − 239.00, − 213.24, and − 290.08, respectively), suggesting direct interference with receptor-mediated signaling cascades. This study presents the first transcriptome-level analysis of the immunoregulatory network of amphibian cathelicidins, providing insights for developing novel peptide-based therapeutics against drug-resistant infections and inflammatory disorders.
Discovery and structural characterization of newly identified mutations in the HBx gene of chronic HBV patients
Single molecule nanopore counting assay targeting small extracellular vesicle cargo for non-invasive monitoring of cerebral organoid development and health
Abstract Organoids are three-dimensional tissue cultures intended to replicate in vivo organs such that their function can be analyzed for applications in drug discovery, diagnostics, and research. This requires the ability to assess organoid health, development, and function on the cellular and molecular level, possibly frequently and over long periods of time. Here, we report an assay for monitoring organoid development and health by tracking the molecular cargo of small extracellular vesicles (sEV) with an integrated nanopore sensor chip. Specifically, we implement amplification-free and label-free quantification of the organoid stress marker ENO1 produced by cerebral organoid tissue. We demonstrate that mRNA levels measured non-invasively in sEVs are representative of the amounts measured by PCR measurements of the tissue cells. We also quantify the ENO1 RNA load in sEVs over the course of 15 weeks and show that ENO1 expression levels are correlated with other physiological parameters such as organoid glucose consumption. These results illustrate the capability of single molecule nanopore sensors for providing simple, continuous, quantitative assessment of organoids’ phenotypes on the molecular level. This approach can be expanded to other molecular biomarkers such as protein transcripts, multiplexed analysis, and fully integrated in-line analysis in an automated tissue culture platform.