Browse Articles

Prognostic molecular subtype reveals the heterogeneity of tumor immune microenvironment in gastric cancer

A suppression-modification gene drive for malaria control targeting the ultra-conserved RNA gene mir-184

Abstract Gene drive technology presents a promising approach to controlling malaria vector populations. Suppression drives are intended to disrupt essential mosquito genes whereas modification drives aim to reduce the individual vectorial capacity of mosquitoes. Here we present a highly efficient homing gene drive in the African malaria vector Anopheles gambiae that targets the microRNA gene mir-184 and combines suppression with modification. Homozygous gene drive (miR-184D) individuals incur significant fitness costs, including high mortality following a blood meal, that curtail their propensity for malaria transmission. We attribute this to a role of miR-184 in regulating solute transport in the mosquito gut. However, females remain fully fertile, and pure-breeding miR-184D populations suitable for large-scale releases can be reared under laboratory conditions. Cage invasion experiments show that miR-184D can spread to fixation thereby reducing population fitness, while being able to propagate a separate antimalarial effector gene at the same time. Modelling indicates that the miR-184D drive integrates aspects of population suppression and population replacement strategies into a candidate strain that should be evaluated further as a tool for malaria eradication.

Machine learning approach for optimizing usability of healthcare websites

Chromosomal instability in human trophoblast stem cells and placentas

Mixed matrix membrane of poly(4-methyl-1-pentyne) and ZIF-8 for enhanced CO2 separation over H2 and CH4

An individual-based modelling study estimating the impact of maternity service delivery on health in Malawi

Abstract Maternal and perinatal morbidity and mortality remain high in Malawi, partially due to gaps in the coverage and quality of health services. We developed an individual-based model of maternal and perinatal health and healthcare in Malawi, situated in a ‘whole-health system, all-disease’ framework ( Thanzi La Onse ). We modelled sixteen scenarios estimating the impact of current and improved coverage and quality of antenatal, intrapartum, and postnatal services from 2023 to 2030. Whilst current service delivery is inferred to avert morbidity and mortality, the largest reductions in the stillbirth, maternal and neonatal mortality rates were observed when the use and quality of all services was maximised concurrently (a 10%, 52% and 57% reduction respectively). When services were considered in isolation, generally, increased coverage without quality improvement did not impact mortality or DALYs. In only three scenarios was a sufficient reduction in neonatal mortality observed to achieve target 3.2 of the United Nation’s Sustainable Development Goals (SDG), and in no scenarios was a reduction in maternal mortality sufficient to achieve SDG target 3.1 observed, reaffirming that system wide investments are essential to achieve these goals.

Surface modification of electrospun polycaprolactone and zein using cold atmospheric plasma for tissue engineering applications

Single-cell RNA sequencing dissects the immunosuppressive signatures in Helicobacter pylori-infected human gastric ecosystem

Trust-driven approach to enhance early forest fire detection using machine learning

Chern networks: reconciling fundamental physics and device engineering

The effect of preparation process on combustibility of Robinia pseudoacacia charcoal and its structure-performance relationship

A specific microbial consortium enhances Th1 immunity, improves LCMV viral clearance but aggravates LCMV disease pathology in mice

Abstract Anti-viral immunity can vary tremendously from individual to individual but mechanistic understanding is still scarce. Here, we show that a defined, low complex bacterial community (OMM12) but not the general absence of microbes in germ-free mice leads to a more potent immune response compared to the microbiome of specific-pathogen-free (SPF) mice after a systemic viral infection with LCMV Clone-13. Consequently, gnotobiotic mice colonized with OMM12 have more severe LCMV-induced disease pathology but also enhance viral clearance in the intestinal tract. Mechanistically, single-cell RNA sequencing analysis of adoptively transferred virus-specific T helper cells and endogenous T helper cells in the intestinal tract reveal a stronger pro-inflammatory Th1 profile and a more vigorous expansion in OMM12 than SPF mice. Altogether, our work highlights the causative function of the intestinal microbiome for shaping adaptive anti-viral immunity with implications for vaccination strategies and anti-cancer treatment regimens.

Optimization of tangential interior brushless DC motor rotor for hybrid vehicles

Deciphering distinct genetic risk factors for FTLD-TDP pathological subtypes via whole-genome sequencing

Robust optical design of high-contrast vehicle headlamps with cylindrical lens array and inverted triangular beam pattern

T-bet+CD8+ T cells govern anti-PD-1 responses in microsatellite-stable gastric cancers

BPIFB4 rs4339026 A > G polymorphism impacts COPD susceptibility in the Kashi population, China

Blockchain-based isotopic big data-driven tracing of global PM sources and interventions

Abstract Tracing sources and assessing intervention effectiveness are crucial for controlling atmospheric particulate matter (PM) pollution. Isotopic techniques enable precise top-down tracing, but the absence of long-term, global-scale multi-compound isotopic data limits comprehensive analysis. Here, we establish a blockchain-based isotopic database, compiling 34,815 isotopic fingerprints of global PM and its emissions from 1,890 pollution events across 66 countries. This allows retrospective analysis and predictions, revealing that PM sources are distinct, dynamically changing over time, and often asynchronous with interventions. Additionally, we estimate source contributions to PM2.5 and its compounds, highlighting the increasing impact of biomass burning. Furthermore, projections indicate that by 2100, PM levels may decline to 5.38 ± 0.16 μg/m³ in the Americas and 13.9 ± 1.82 μg/m³ in Asia under climate mitigation scenarios but will still exceed WHO guidelines without further controls on natural emissions. Guiding future interventions with isotopic big data is essential for addressing air pollution challenges.

Development and application of tail brush elastic test device

Wild and domesticated animal abundance is associated with greater late-Holocene alpine plant diversity

Abstract In the face of human land use and climate dynamics, it is essential to know the key drivers of plant species diversity in montane regions. However, the relative roles of climate and ungulates in alpine ecosystem change is an open question. Neither observational data nor traditional palaeoecological data have the power to resolve this issue over decadal to centennial timescales, but sedimentary ancient DNA (sedaDNA) does. Here we record 603 plant taxa, as well as 5 wild, and 6 domesticated mammals from 14 lake sediment records over the last 14,000 years in the European Alps. Sheep were the first domesticated animals detected (at 5.8 ka), with cattle appearing at the early Bronze Age (4.2 ka) and goats arriving later (3.5 ka). While sheep had an impact similar to wild ungulates, cattle have been associated with increased plant diversity over the last 2 ka by promoting the diversity of forbs and graminoids. Modelling of the sedaDNA data revealed a significantly larger effect of cattle and wild ungulates than temperature on plant diversity. Our findings highlight the significant alteration of alpine vegetation and the entire ecosystem in the Alps by wild and domesticated herbivores. This study has immediate implications for the maintenance and management of high plant species diversity in the face of ongoing anthropogenic changes in the land use of montane regions.