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Linker-GPT: design of Antibody-drug conjugates linkers with molecular generators and reinforcement learning
An in vitro study on the efficacy of nanoparticles and nanocomposites as coating materials on surgical sutures
Abstract Upgrading the surgical sutures, as the main wound closure device, is essential. The evolution of bacterial resistance and the plummeting of antibiotics have directed research toward augmented sutures. Nanotechnology has provided answers to these concerns. The use of bacterial isolates as bio-factory for synthesized silver nanoparticles (AgNPs) and silver nanocomposites via a one pot ex situ method provides environmentally friendly silver nanocomposites in addition to the use of chitosan and polyvinyl alcohol polymers as carriers. Transmission electron microscopy (TEM) and zeta potential analysis revealed spherical negatively charged AgNPs. These nanoparticles and nanocomposites were used as coatings for absorbable vicryl and nonabsorbable silk surgical sutures. Atomic force microscopy (AFM) 3D images of these coated sutures showed a significant decrease in surface roughness with improved surface topography, specifically with chitosan-silver (CS-Ag) vicryl coated sutures with effective attachment of the nanocomposite and nanoparticles thin film on the suture surface. Field emission scanning electron microscopy with energy dispersive x-ray spectroscopy (FE-SEM/EDX) analysis showed the significant presence of the thin film of coating materials on the surface of the sutures and the significant elemental presentation of Ag. Vicryl and silk coated CS-Ag sutures showed significant antibacterial and antibiofilm activities against both gram positive and gram-negative bacterial isolates. AgNPs coated silk and vicryl sutures recorded the lowest amounts of Ag ions at 0.03–0.45 ppm released after 14 days, while polyvinyl alcohol-silver (PVA-Ag) coated ones showed the highest rates at 0.75–0.93 ppm.
Analysis of spatiotemporal evolution and driving factors of ecological environment quality in the Ili-Balkhash Lake Basin in Central Asia
Integrative network pharmacology and experimental validation of anti-inflammatory triterpenoids from hawthorn leaves
Agricultural production and international trade analysis of competitiveness between natural and conventional dyes in the textile industry
Optimal scheduling of integrated energy system with gas–liquid phase change carbon dioxide energy storage considering multi-layer low-carbon benefits
Stock composition of cryptic Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) in the Restigouche River and estuary, Canada
A multi layered encryption framework using intuitionistic fuzzy graphs and graph theoretic domination for secure communication networks
Ionic organic cage as a versatile platform for immobilizing chemical and enzymatic sites for chemoenzymatic catalysis
A heterozygous CEBPA mutation disrupting the bZIP domain in a RUNX1 and SRSF2 mutational background causes MDS disease progression
Abstract Myelodysplastic syndrome disease (MDS) is caused by the successive acquisition of mutations and thus displays a variable risk for progression to AML. Mutations in CEBPA are commonly associated with a high risk of disease progression, but whether they are causative for AML development is unclear. To analyse the molecular basis of disease progression we generated MDS patient-derived induced pluripotent stem cells from a low risk male patient harbouring RUNX1 / SRSF2 mutations. This experimental model faithfully recapitulates the patient disease phenotypes upon hematopoietic differentiation. Introduction of a frameshift mutation affecting the C/EBPα bZIP domain in cells from low-risk stages mimicks disease progression by reducing clonogenicity of myeloid cells, blocking granulopoiesis and increasing erythroid progenitor self-renewal capacity. The acquisition of this mutation reshapes the chromatin landscape at distal cis-regulatory regions and promotes changes in cellular composition as observed by single cell RNAseq. Mutant C/EBPα is therefore causative for MDS disease progression. Our work identifies mutant CEBPA as causative for MDS disease progression, providing a new isogenic MDS experimental model for drug screening to improve diagnostic and therapeutic strategies.
Total-area world-record efficiency of 27.03% for 350.0 cm2 commercial-sized single-junction silicon solar cells
De Novo Design of Structure‐Tunable Multivalent Targeting Chimeras for Tumor‐Targeted PD‐L1 Degradation and Potentiated Cancer Immunotherapy
Abstract Targeted protein degradation (TPD) technology holds significant potential for modulating protein homeostasis and treating diseases. However, current methods for degrading membrane proteins highly depend on the lysosome‐targeting ligands or membrane receptors. In this study, we present a set of multivalent targeting chimeras (multi‐TACs) for tumor‐specific degradation of programmed death ligand 1 (PD‐L1) on the surface of the tumor cell membrane. The multi‐TACs are synthesized by copolymerization of small‐molecule PD‐L1 inhibitor BMS‐1 with acid‐responsive monomers. The chemical structures of the multi‐TACs are optimized by investigating the correlation between PD‐L1 degradation efficacy and the key parameters, including acid‐sensitive moieties, BMS‐1 valency, and spacer length. Mechanistic study reveals that the multi‐TACs highly efficiently degrade PD‐L1 on the surface of tumor cells via the adsorption‐mediated endocytosis and lysosomal degradation pathways, which differ from the reported strategies for membrane protein degradation. The outperformed multi‐TAC GG56 with tumor extracellular acidity and enzyme‐sensitivity dramatically reduces PD‐L1 levels and suppresses tumor growth in mouse models of B16‐F10 melanoma and 4T1 breast tumors. Furthermore, GG56 serves as a versatile nanoplatform for combinatory chemo‐immunotherapy and radio‐immunotherapy of 4T1 breast tumor by co‐delivery of chemotherapeutic and radio‐sensitizer, respectively.
Antibacterial activity of a conventional hydrogel and a nanoparticle based hydrogel containing Satureja khuzestanica essential oil
PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling
Abstract In this study, we investigated the potential relationship between the mitochondrial network and the microbiome using wild-type and skeletal muscle-specific PGC-1α (Pparg coactivator 1 alpha) overexpressing mice, both with and without exercise training. Basal PGC-1α levels were significantly higher in the skeletal muscle (J Physiol Biochem 80:329–335, 2024. https://doi.org/10.1007/s13105-024-01006-1 ) and, notably, in the colon, which is anatomically proximal to the microbiome. However, no significant changes were observed in cell signaling or mitochondria-related proteins within the colon. On the other hand, mitochondrial H₂O₂ production in the colon decreased in the PGC-1α overexpressing group. The relative abundance of several bacterial taxa differed between wild-type and PGC-1α overexpressing groups at baseline condition, indicating a shift in the microbiome milieu probably to cope with the increased metabolism, enhanced short-chain fatty acid utilization, and improved endurance capacity. Ten weeks of exercise training differentially modulated the host microbiome in PGC-1α overexpressing and wild-type mice, facilitating adaptations to a broad range of exercise-induced challenges. The results of this study provide new insights into the possible cross-talk between mitochondria and the microbiome.
Biosynthesis of zinc oxide nanoparticles using Moringa oleifera leaf extract, probing antibacterial and antioxidant activities
Periplocin induces necroptosis in papillary thyroid carcinoma through DR4 mediated RIPK3 and MLKL signaling
Multi-step finite element simulation for clear aligner space closure: a proof-of-concept compensation protocol
Engineering M13 bacteriophage to display HER2 mimotopes on pVIII for vaccine development
Negative life events, sleep quality and depression in university students
Abstract The rate of depression among university students is increasing. University students experiencing negative life events are at risk of developing depression, which could further decrease their academic engagement. In addition, negative life events are often accompanied by sleep problems. This study used a cross-sectional design to explore the complex relationships among negative life events, sleep quality, and depression in university students, with a particular focus on whether sleep quality serves as a mediating factor in the association between negative life events and depression. Three self-report scales were completed by 828 participants recruited from three universities. The results revealed that negative life events and sleep quality had a significant effect on depression separately and that sleep quality played a mediating role in the relationship between negative life events and depression in university students. Our study contributes to a deeper understanding of the relationships among negative life events, sleep quality, and depression and has important implications for the prevention and intervention of depressive symptoms in university students. By acknowledging the role of negative life events, educators and counselors can be more proactive in identifying students who may be struggling and providing them with the necessary support. By understanding the mediating role of sleep quality, university students can more effectively recognize and manage their mental health needs and cultivate resilience strategies to handle stressors to reduce their risk of developing depressive symptoms.