Browse Articles

Gonadotropin-releasing hormone regulates transcription of the inhibin B co-receptor, TGFBR3L, via early growth response one

Imaging‐Based High‐Content Screening with Clickable Probes Identifies XPB Inhibitors

Abstract High‐content screening (HCS) has become a powerful tool in drug discovery; however, its reliance on indirect readouts and surrogate markers limits HCS's ability to directly assess drug‐protein interactions at endogenous levels, particularly in subcellular contexts. Here, we report an approach to address these limitations by combining confocal imaging‐based HCS and bio‐orthogonal labeling with clickable probes. As a proof‐of‐concept, we synthesized a probe Triptolide‐alkyne (TL‐alk) that rapidly and specifically labels xeroderma pigmentosum type B (XPB), a critical protein in nucleotide excision repair (NER). Probe‐labeled XPB was conjugated to TAMRA to visualize the occupation of active sites, and EGFP and DAPI signals indicated XPB expression in the nucleus. Such a colorimetric HCS assay enabled the direct and precise measurement of drug occupancy rates in nuclear XPB of live cells. With this platform, pelitinib was identified as a novel ligand to bind XPB out of 1874 compounds containing. Food and Drug Administration (FDA)‐approved drugs. Pelitinib formed a covalent bond with cysteine residue 342 of XPB, suppressed XPB's ATPase activity, impaired NER, and synergistically enhanced chemotherapy. This study not only overcomes limitations of HCS, but also demonstrates the transformative potential of bio‐orthogonal labeling, such as in integration with HCS technologies, offering a novel framework for drug discovery targeting challenging protein systems.

Deuterated Multi‐Resonance Thermally Activated Delayed Fluorescence Emitters for Pure‐Green Organic Light‐Emitting Diodes with CIE Coordinates of (0.16, 0.75) and Long Lifetimes

Abstract Multi‐resonance thermally activated delayed fluorescence (MR‐TADF) emitters, especially high‐order B/N MR‐TADF emitters, offer new opportunities in obtaining wide‐color‐gamut, high color purity, and highly efficient organic light‐emitting diodes (OLEDs). Extending the device durability is still very challengeable for these emitters. Deuteration of emitters to slow down their degradation and lengthen device lifespan is a promising strategy. However, how to access deuterated high‐order MR‐TADF emitters remains to be explored. Herein, a synthetic protocol of high‐order B/N products is proposed using deuterated starting materials and sequential borylation. Combining with fine‐tuning electronic effect through adjusting position of tert ‐butyl ( t Bu) substituents, we successfully obtained deuterated triboron MR‐TADF material ω’‐DABNA‐D that delivers precisely tuned pure‐green emission with close‐to‐unity quantum yield and rapid reverse intersystem crossing (RISC). The resulting binary OLED approaches the BT.2020 standard with CIE coordinates of (0.16, 0.75) and demonstrates impressive external quantum efficiency (EQE) of 34.6% at maximum and 30.7% at 1000 cd m −2 . Additionally, an Ir(ppy) 3 ‐sensitized device shows excellent operational stability, with an extrapolated lifetime (LT 80 ) of 6078 h at an initial luminance of 1000 cd m −2 . This study hence offers a promising methodology toward high‐performance OLED with long device lifetime.

Lead-free perovskite KCsSnI1.7Cl1.3 material exhibiting superior photocatalytic antimicrobial activity

Abstract The development of environmentally friendly and highly efficient materials is critical for next-generation antibacterial and optoelectronic applications. In this study, we present the successful synthesis of a novel lead-free perovskite, KCsSnI1.7Cl1.3, via a rapid and scalable chemical bath deposition method at 150 °C for just 5 min. The resulting film features well-defined orthorhombic, pyramid-like crystals with uniform grain sizes (800–1000 nm) and a compact, pinhole-free morphology. Remarkably, the material exhibits strong optical absorption up to 800 nm, positioning it as a promising candidate for hot electron generation and light-driven applications. KCsSnI1.7Cl1.3 also demonstrated outstanding antibacterial performance, showing broad-spectrum activity against multiple bacterial strains. The highest inhibition was recorded against Staphylococcus aureus, with inhibition zones increasing from 17 mm in the dark to 35 mm under UV illumination—highlighting its efficient photocatalytic response. The antibacterial effect followed the order: S. aureus > B. subtilis > E. coli > Salmonella sp., and was significantly enhanced with increasing concentrations (100–400 ppm). No inhibition was observed in control wells, confirming the selective activity of the material. With its lead-free composition, strong light-harvesting ability, and exceptional antibacterial properties, KCsSnI1.7Cl1.3 emerges as a highly promising material for future applications in antibacterial coatings, water purification systems, and advanced optoelectronic devices. Its green synthesis, scalability, and multifunctionality offer a sustainable pathway toward real-world deployment in environmental and biomedical fields.

Identification and characterization of nanobodies specific for the human ubiquitin–like ISG15 protein

Janus‐Type Photophysics of Rotational Isomers in a Diphenylanthracene Dimer

Abstract Organic molecular dimers serve as valuable model systems for manipulating and exploring interchromophore interactions. However, the structural flexibility introduced by linker or chromophore rotation gives rise to conformational isomers with distinct atomic arrangements, complicating the interpretation of photophysical processes. In this study, we investigate 9,9′,10,10′‐tetraphenyl‐2,2′‐bianthracene ( TPBA ) to elucidate the distinct electronic characteristics of its two primary rotational isomers: syn ‐ and anti ‐TPBA . To address the “Janus‐type” photophysical behavior of these isomers, we employed a comprehensive suite of static and time‐resolved spectroscopic techniques, including excitation‐wavelength‐dependent time‐resolved photoluminescence, transient absorption, and time‐resolved electron paramagnetic resonance, complemented by theoretical calculations. syn ‐ and anti ‐TPBA exhibit markedly different emissive properties and charge‐transfer characteristics, reflecting their unique exciton coupling behaviors. Additionally, they showcase distinct triplet formation rates and exhibit environment‐dependent triplet formation mechanisms. This in‐depth study of the Janus‐like electronic properties of TPBA underscores the critical role of conformational isomerism in organic molecular dimers. Neglecting these structural variations can obscure the true photophysical landscape and lead to misinterpretations of mechanistic processes, highlighting the necessity of considering conformational heterogeneity in molecular design and photophysical studies.

Profiling plasma protease activity with charge-changing peptides enables detection and classification of gastrointestinal cancers

Abstract Early detection of gastrointestinal (GI) cancers—including colorectal cancer (CRC), gastric cancer (GC), and esophagogastric junction cancer (EGJC)—is essential for improving patient outcomes. However, current diagnostic methods such as endoscopy and colonoscopy are invasive, costly, and not widely accessible. Proteases are elevated in many cancers and are detectable in peripheral blood, making them promising candidates for noninvasive diagnostic strategies. We employed a six-probe charge-changing peptide (CCP) panel to profile cancer-associated protease activity in human plasma. Each CCP undergoes a charge shift upon cleavage by a specific protease, enabling detection via gel electrophoresis. Plasma samples from GI cancer patients (CRC, GC, EGJC; N = 68) and healthy controls (HC; N = 31) were analyzed. Protease activity profiles were analyzed using statistical tests, principal component analysis, and binary logistic regression (LR) models trained on the most informative probes. Model performance was evaluated through repeated cross-validation. Distinct protease activity profiles were observed among CRC, upper GI cancers (UGIC; GC + EGJC), and HC groups. Probe designed to be cleaved by cathepsin B showed the strongest discrimination between cancer and control samples, while probes designed to be cleaved by ubiquitin-specific peptidase 15 and plasmin were identified as the most informative subtype-specific markers for UGIC and CRC, respectively. LR models built on these single probes demonstrated excellent diagnostic performance, with AUCs exceeding 0.95, and both sensitivity and specificity greater than 90%. Our findings highlight CCP-based protease profiling as a minimally invasive, accurate, and scalable method for GI cancer detection and classification. This platform holds strong potential for clinical application in cancer screening, pending further validation in larger, independent cohorts.

Gut microbiota–derived metabolite trimethylamine N-oxide alters the host epigenome through inhibition of S-adenosylhomocysteine hydrolase

Cooperative Activation of CO and Pyridine by an Aluminum(I) Complex Ligated with a Silylene–Borane Ligand

Abstract Cooperative main‐group systems based on alumylenes are highly attractive due to their potential for activating and transforming inert chemical bonds and small molecules. However, their development has been hindered by the scarcity of suitable supporting ligands. Herein, we report the synthesis of an amphiphilic carboranyl silylene–borane ligand ( 1 ) and demonstrate its effectiveness in stabilizing an aluminum(I) complex 2 . Complex 2 has been unambiguously characterized by spectroscopic analysis, X‐ray diffraction analysis, and DFT calculations, which reveals a unique structure featuring both silicon(II)→aluminum(I) and aluminum(I)→boron(III) donor–acceptor bonds. The synergistic interplay between the silylene–borane ligand and the aluminum(I) center in 2 drives its unusual reactivity toward CO and pyridine activation, facilitating cleavage of the C≡O bond and dearomatization of pyridine.

Analysis of the seepage-diffusion law and transformation mechanism in the CO2 flow adsorption test under various laminae

Repeated activation of Gαq has a detrimental impact on C. elegans in an age-dependent manner

Solitary and soliton solutions of the nonlinear fractional Chen Lee Liu model with beta derivative

Polyserine–tau interactions modulate tau fibrillization

Atomic‐Scale Mott–Schottky Analogy in SnCu Nanoalloy Promote High‐Efficiency Urea Electrosynthesis at Ultralow Potential

Abstract Electrocatalytic urea synthesis from CO 2 and NO 3 − offers a sustainable strategy to address environmental challenges and growing urea demand. However, current systems suffer inefficient C‐N coupling due to poor selectivity toward critical C/N‐intermediates. Herein, we engineered atomic‐scale Mott–Schottky analogy in SnCu nanoalloy to create electron‐enriched Cu sites, enabling remarkable urea production through quadruple synergy. Sn 2 Cu delivered exceptional urea yield (28.9 mmol h −1 g cat. −1 ) with 46.7% Faradaic efficiency (FE) in H‐cell, while demonstrating practical potential with superior catalytic performance (yield: 72.6 mmol h −1 g cat. −1 , FE: 41.3%, stability: 60 h) at −0.52 V in flow cell. In‐situ synchrotron radiation‐Fourier transform infrared spectroscopy and theoretical calculations revealed electron‐enriched Cu active sites enhanced CO 2 /NO 3 − co‐adsorption and *CO coverage, while steering reaction pathway toward *CO‐*NHO coupling and suppressing hydrogen evolution, thereby reducing rate‐determining step energy barrier and prioritizing C‐N coupling. This work develops a structure–adsorption‐reactivity framework, providing fundamental guidance for advanced urea electrocatalyst design.

Improving lung cancer detection with enhanced convolutional sequential networks

WTAP-mediated m6A methylation of circRNA_404908 promotes esophageal squamous cell carcinoma progression

Atomic‐Level Design of Acid–Base Pairs in Oxides for Selective Catalytic Reduction of Nitrogen Oxides with Ammonia

Abstract Selective catalytic reduction of nitrogen oxides (NO x ) with NH 3 (NH 3 ‐SCR) poses considerable potential in the abatement of NO x emissions. However, the efficient adsorption and speedy reaction of reactants following the specific mechanism in a favorable way is still a challenge for enhancing catalysis. Herein, we propose the strategy aimed at adjusting electronic properties of Ce‐O v ‐W acid–base pairs through constructing oxygen vacancies on Ce/WO x , thereby fostering SCR activity. Experimental and theoretical results reveal that Ce‐O v ‐W acid–base pairs not only provide more Ce 3+ sites for promoting the reactivity of adsorbed NO, but also accelerate the reaction between NH 3 and gaseous NO owing to the generation of W 5+ species with superior surface acidity, which enhance Langmuir–Hinshelwood and Eley–Rideal mechanisms, respectively. Consequently, the designed catalysts achieve over 90% NO x conversion above 250 °C and exhibit higher activity than normal Ce/WO 3 and V/W‐TiO 2 commercial catalysts, with anti‐poisoning of SO 2 and H 2 O under harsh working conditions, expecting to provide the guidance for promoting de‐NO x industrial application.

Assessment of classroom design for physical education using COCOSO algorithm and modified Sugeno Weber aggregation operators

A three-stage assembly program governing pancreatic, plasma, pituitary, and bone secretory cell differentiation: A strategy to augment protein delivery

Light‐Induced Fe‐LMCT Catalysis for Redox‐Coupled Conversion of NO _x and SO ₂ Mixture

Abstract The coexistent nitrogen oxides (NO x ) and sulfur dioxide (SO 2 ) in flue gas pose inherent challenges for simultaneous removal due to their disparate reactivities. Conventional sequential treatments for their simultaneous removal face major issues of catalyst deactivation and byproduct generation. Here, we develop a subtle strategy using light‐induced ligand‐to‐metal charge transfer (LMCT) catalysis with Fe(II) ethylenediaminetetraacetic acid (EDTA‐Feᴵᴵ) to achieve redox‐coupled conversion of NO and SO 2 mixtures. LMCT excitation in EDTA‐Fe II induces directional charge separation under irradiation, routing photogenerated electrons (e⁻) to Feᴵᴵ for driving selective NO‐to‐N 2 conversion (selectivity: 99.89%), while photogenerated holes (h + ) oxidize SO 2 to SO 4 2 ⁻ (selectivity: 96.34%). This spatial segregation of redox pathways suppresses N 2 O generation, enabling continuous operation with 90.3% NO and nearly 100% SO 2 removal efficiency. Mechanism studies reveal the LMCT‐enhanced charge transfer from carboxyl/amino groups to Fe centers, while in situ EPR confirms the •SO 3 2 ⁻ radical‐mediated h + scavenging that accelerates charge separation and utilization. This work establishes Fe‐LMCT catalysis as a sustainable platform for gas‐phase pollutants remediation, achieving unprecedented selectivity through precise redox pathway control.