Browse Articles

Diet quality of Chilean schoolchildren: How is it linked to adherence to dietary guidelines?

Introduction In Chile, the prevalence of overweight among schoolchildren over the age of 5 is approximately 50%, one of the highest in the Americas region. This is largely influenced by the presence of inadequate food environments, characterized by limited access to healthy foods and a high availability of highly processed foods (HPF). This study analyzes food consumption in Chilean schoolchildren and the association of this with the Chilean Dietary Guidelines Based on Foods (DGBF) and the HPF consumption. Material and methods The sample (1,094 schoolchildren) was obtained from the baseline of the FONDEF IT18I0016 project, in Santiago-Chile. Anthropometry and socioeconomic data were measured. Dietary intake was assessed using a validated semi-quantitative frequency survey featuring images of food groups aligned with DGBF, as well as HPF. All data were collected by trained professional nutritionists. Associations between dependent and independent variables, and potential confounding factors were calculated using logistic regression models with stepwise backward/forward. A p < 0.05 was accepted as significant, using Stata 16.0. Results Most of the students (54.1%) were with malnutrition by excess and 20.0% exhibited abdominal obesity. Twenty percent of the students met the recommended intake for DGBF (fruits, vegetables, fish, milk and dairy products, legumes, water) and less than 5% did not consume any HPF. Consuming some sub-groups of HPF in moderate quantities (either no consumption or occasional consumption) increases the likelihood of meeting DGBF: candies and sweets (OR: 0.72; p = 0.04), pies with or without filling (OR: 0.63; p = 0.01), and salted snacks (OR: 0.67; p = 0.02). Conclusion Our results contribute to reaffirm the need to enhance healthy food consumption among schoolchildren and to discourage the intake of HPF, particularly focusing on specific sub-groups of HPF that may increase the risk of displacing the consumption of food groups recommended by the DGBF.

Double Stokes polarimetric microscopy for chiral fibrillar aggregates

Abstract Second harmonic generation (SHG) microscopy is a powerful tool for imaging collagen and other noncentrosymmetric fibrillar structures in biological tissue. Polarimetric SHG measurements provide ultrastructural information about the fibrillar organization in a focal volume (voxel). We present a reduced nonlinear polarimetry method named double Stokes polarimetry (DSP) for quick characterization of chiral $$C_6$$ C 6 symmetry fibers without data fitting that simplifies and speeds up the polarimetric analysis. The method is based on double Stokes-Mueller polarimetry and uses linear and circular incident and outgoing polarization states. The analytical expressions of DSP polarimetric parameters are defined in terms of conventional SHG Stokes vector components. A complex chiral susceptibility (CCS) model is assumed to derive expressions of ultrastructural parameters consisting of the magnitude and phase of molecular complex-valued chiral susceptibility ratio, real-valued achiral ratio, and fiber orientation in a voxel. The ultrastructural parameters are expressed in terms of directly measurable DSP polarimetric parameters. DSP is validated with rat tail tendons sectioned at different orientations. DSP can be applied to investigate the origin of chiral complex-valued susceptibility of collagen, to study modifications of collagen in cancerous tissue, and to map ultrastructural parameters of large areas for whole-slide histopathology.

Host metabolism balances microbial regulation of bile acid signalling

Distinct immune cell infiltration patterns in pancreatic ductal adenocarcinoma (PDAC) exhibit divergent immune cell selection and immunosuppressive mechanisms

Abstract Pancreatic ductal adenocarcinoma has a dismal prognosis. A comprehensive analysis of single-cell multi-omic data from matched tumour-infiltrated CD45+ cells and peripheral blood in 12 patients, and two published datasets, reveals a complex immune infiltrate. Patients have either a myeloid-enriched or adaptive-enriched tumour microenvironment. Adaptive immune cell-enriched is intrinsically linked with highly distinct B and T cell clonal selection, diversification, and differentiation. Using TCR data, we see the largest clonal expansions in CD8 effector memory, senescent cells, and highly activated regulatory T cells which are induced within the tumour from naïve cells. We identify pathways that potentially lead to a suppressive microenvironment, including investigational targets TIGIT/PVR and SIRPA/CD47. Analysis of patients from the APACT clinical trial shows that myeloid enrichment had a shorter overall survival compared to those with adaptive cell enrichment. Strategies for rationale therapeutic development in this disease include boosting of B cell responses, targeting immunosuppressive macrophages, and specific Treg cell depletion approaches.

Synthetic wheat as a new source of flour quality under drought conditions: Associations with solvent retention capacity

Synthetic hexaploid lines are proposed as high-potential germplasm for improving bread wheat by introducing new genes (biotic and abiotic stresses) lost during common wheat evolution. A panel of 99 synthetic and common wheat was studied for quality and grain-related traits and drought tolerance under two different moisture conditions (water stress and normal) during two growing seasons. Results indicated different variations for most traits suggesting that the synthetic hexaploid wheat-derived lines (SHW-DL) panel contains valuable genes for drought tolerance improvement of wheat. Drought stress reduced morphological traits and production but increased protein (Pro), rapid mix test (RMT), and solvent retention capacity (SRC) traits. Synthetic wheat lines were superior with higher grain yield, glutenin, damaged starch, available pentosane, overall water holding capacity, and gluten strength (glutenin and gliadin strength) compared to common wheat making them more suitable for bread-baking. The results showed that solvent retention capacity had a strong capacity to differentiate the quality of wheat genotypes. Correlation analysis indicated that genetic improvement for high-yielding varieties can be achieved by producing more damaged starch, higher water absorption, hardness, and lower gluten strength, and zeleny (ZEL). Selection of superior genotypes using univariate and multivariate methods will be discussed.

Investigating knowledge and attitudes toward genetic testing and counseling among palestinians

One-quarter of freshwater fauna threatened with extinction

Abstract Freshwater ecosystems are highly biodiverse1 and important for livelihoods and economic development2, but are under substantial stress3. To date, comprehensive global assessments of extinction risk have not included any speciose groups primarily living in freshwaters. Consequently, data from predominantly terrestrial tetrapods4,5 are used to guide environmental policy6 and conservation prioritization7, whereas recent proposals for target setting in freshwaters use abiotic factors8–13. However, there is evidence14–17 that such data are insufficient to represent the needs of freshwater species and achieve biodiversity goals18,19. Here we present the results of a multi-taxon global freshwater fauna assessment for The IUCN Red List of Threatened Species covering 23,496 decapod crustaceans, fishes and odonates, finding that one-quarter are threatened with extinction. Prevalent threats include pollution, dams and water extraction, agriculture and invasive species, with overharvesting also driving extinctions. We also examined the degree of surrogacy of both threatened tetrapods and freshwater abiotic factors (water stress and nitrogen) for threatened freshwater species. Threatened tetrapods are good surrogates when prioritizing sites to maximize rarity-weighted richness, but poorer when prioritizing based on the most range-restricted species. However, they are much better surrogates than abiotic factors, which perform worse than random. Thus, although global priority regions identified for tetrapod conservation are broadly reflective of those for freshwater faunas, given differences in key threats and habitats, meeting the needs of tetrapods cannot be assumed sufficient to conserve freshwater species at local scales.

A covalent peptide-based lysosome-targeting protein degradation platform for cancer immunotherapy

Design and analysis of lightweight and robust authentication protocol for securing the resource constrained IIoT environment

The Internet of Things (IoT) is a vast network of devices, sensors, wearables, or any other object capable of processing, storing, sending, and receiving data over an open network channel. This versatility gives IoT numerous applications, one of them being in the industry, also known as the Industrial Internet of Things (IIoT). As IIoT relies on an open network channel for data sharing, it is vulnerable to numerous threats, including side channels, impersonation attacks, and clock synchronization issues for which device authentication becomes crucial. Researchers occasionally proposed numerous authentication protocols using conventional cryptographic methods, identity-based cryptographic techniques, or certificateless methods; however, these protocols either suffer from modular exponentiation partial private key distribution problems or are completed in four to five round-trips during authentication. Therefore, this article presents an Elliptic Curve Cryptographic (ECC)-based efficient technique that emerges as a significant solution, addressing the certificate revocations, overheads problem, and the partial private key distribution problem of identity-based cryptography, respectively. The security of the proposed ECC-based protocol is of utmost importance in addressing all the known vulnerabilities in IIoT, freeing the industrial system from the urgency and the issue of data breaches. Its potential to instil a sense of security and confidence in IIoT deployment is crucial in improving user trust. Upon comparing the proposed protocol with state-of-the-art schemes, the result demonstrated that the proposed protocol enhanced 51.44% in terms of communication costs and 91.88% in terms of computation costs. So, it is recommended for practical implementation due to its fast and provable secure nature, making the industry feel confident and safe about its implementation.

Associations between nutrient intake and osteoarthritis based on NHANES 1999 to 2018 cross sectional study

Parameterized resetting model captures dose-dependent entrainment of the mouse circadian clock

Abstract The phase response curve (PRC) represents the time-dependent changes in circadian rhythm phase following internal or external stimuli. However, this time dependence complicates PRC measurement and quantification owing to its variable shape with changing stimulus intensity. Our previous work demonstrated that resetting a desynchronized circadian clock (singularity response, SR) simplifies the analysis by requiring only amplitude and phase parameters. In this study, we construct a comprehensive model for phase resetting in the mouse circadian clock by converting PRCs into SR parameters. We analyze single-cell PRCs and show that the SR amplitude parameters for different stimulus concentrations follow the Hill equation. Additionally, the model predicts the combined effects of multiple stimuli and pre-treatment (background) on phase response by simple addition or subtraction of individual SR parameters. Experimental validation using SR measurements in mouse cells and tissues confirms the model’s accuracy. This study demonstrates that SRs facilitate PRC quantification and reveal simple rules governing phase resetting properties under various conditions using SR parameters.

Over-the-counter carrageenan-based sprays may interfere with PCR testing of nasopharyngeal swabs to detect SARS-CoV-2

Carrageenan-containing nasal sprays, available over-the-counter (OTC), are often marketed as having anti-viral effects. Carrageenan belongs to the glycosaminoglycan family alongside heparin, and heparin is known to inhibit real-time quantitative polymerase chain reaction (RT-qPCR) in nasopharyngeal swabs used to detect SARS-CoV-2. As heparin and carrageenan share structural similarities, this work aimed to investigate the interferent effect of carrageenan on RT-qPCR for SARS-CoV-2 detection across 4 different diagnostic platforms. This work demonstrated that in the presence of carrageenan samples return inaccurate and invalid results on the Seegene STARlet, while qualitative accuracy was maintained on the Cepheid GeneXpert, Roche Cobas LIAT, and Hologic Panther Aptima. Evidence of carrageenan interference on SARS-CoV-2 testing was consistent across two OTC brands and research-grade reconstituted iota-carrageenan, with 80% of results returning invalid regardless of the carrageenan formulation added to the samples. Further, a preliminary in vivo interference study demonstrated an increased Ct value within 15 minutes of carrageenan dosage, with Ct values restored 60 minutes post-application. A direct comparison of carrageenan- and heparin-mediated PCR interference demonstrated that carrageenan PCR interference occurs to a lesser degree, but is not reversible by the addition of heparinase I. As carrageenan is available OTC, interference with PCR testing that causes an increase in false negative results could lead to accidental spread of disease and could therefore have significant public health impacts on community testing of respiratory infectious diseases via PCR.

Effects of time restricted feeding combined with Lacto Ovo vegetarian diet on metabolic associated fatty liver disease management: a randomized clinical trial

Copper-dependent halogenase catalyses unactivated C−H bond functionalization

A distinct double-ring LH1–LH2 photocomplex from an extremophilic phototroph

Beyond pleasurable and meaningful: Psychologically rich entertainment experiences

Entertainment experiences have been conceptualized as hedonic (pleasurable) or eudaimonic (meaningful), mirroring the hedonic and eudaimonic components of psychological well-being. However, psychologists have proposed a third component of well-being: psychological richness, which is characterized by variety, novelty, and interest. In this paper we explore the role of psychological richness in film and television entertainment experiences. Two studies, an experience sampling study (n = 28) and a survey (students in the US, n = 247 and general population in Germany, n = 289) show the prevalence of experience of psychological richness during media use and its positive relationship with well-being. A replication with a different scale (n = 291) demonstrates that psychologically rich entertainment experiences may have been previously been conflated by some measures of eudaimonic entertainment. Incorporating psychologically rich entertainment experiences as a third addition to hedonic and eudaimonic experiences can increase the intervention potential of media used to enhance well-being.

Assessing type-2 diabetes risk based on the Indian diabetes risk score among adults aged 45 and above in India

Probing spectral features of quantum many-body systems with quantum simulators

Abstract The efficient probing of spectral features is important for characterising and understanding the structure and dynamics of quantum materials. In this work, we establish a framework for probing the excitation spectrum of quantum many-body systems with quantum simulators. Our approach effectively realises a spectral detector by processing the dynamics of observables with time intervals drawn from a defined probability distribution, which only requires native time evolution governed by the Hamiltonian without ancilla. The critical element of our method is the engineered emergence of frequency resonance such that the excitation spectrum can be probed. We show that the time complexity for transition energy estimation has a logarithmic dependence on simulation accuracy and how such observation can be guaranteed in certain many-body systems. We discuss the noise robustness of our spectroscopic method and show that the total running time maintains polynomial dependence on accuracy in the presence of device noise. We further numerically test the error dependence and the scalability of our method for lattice models. We present simulation results for the spectral features of typical quantum systems, either gapped or gapless, including quantum spins, fermions and bosons. We demonstrate how excitation spectra of spin-lattice models can be probed experimentally with IBM quantum devices.

Mechanical behavior of synthetic fiber ropes for mooring floating offshore wind turbines

The continuous development of floating wind turbine technology is pushing platforms further offshore into deeper seas. Consequently, the water depth required for mooring is increasing, demanding higher standards for the mooring system. This study explores the mechanical properties, including wear resistance and quasi-static stiffness, of nylon, polyester, and high-strength polyethylene mooring ropes through experimental design, aiming to address the challenges faced by floating offshore wind turbines (FOWT) in mooring line safety. In conducting yarn-on-yarn abrasion tests, materials, twist, marine lubricants, and dry and wet environments were chosen as research variables to analyse and compare the wear and tear frequency of sample ropes. The study found that the degree of wear on ropes composed of different materials is affected differently by twist and dry and wet environments, and the application of marine lubricants can significantly extend the friction fracture cycle of yarns. To study the tensile properties and static stiffness characteristics of polyester and nylon ropes, the cables were divided into three stages: preloading, initial installation, and ageing. The strain and reversible elongation of the cables at each stage were analysed, and an empirical formula for quasi-static stiffness considering the creep coefficient of the ropes was established. The study found that the ropes had reversible elongation after being left stationary, the more thorough the running-in, the smaller the inherent deformation, and the more stable the structure. The static stiffness of the cables increased with the loading time and force; however, it eventually approached a constant value, and the stiffness of polyester cables was found to be greater than that of nylon ropes. These results provide a solid theoretical foundation and practical guidance for the material selection of FOWT cables, which contributes to advancing the application and development of FOWT in renewable energy.

Clinical and measurement variables affecting halitosis intensity following cysteine challenge test