Browse Articles

Structural basis of siderophore export and drug efflux by Mycobacterium tuberculosis

Machine learning selection of basement membrane-associated genes and development of a predictive model for kidney fibrosis

Structural insights into transmembrane helix S0 facilitated RyR1 channel gating by Ca2+/ATP

Sex differences in the relationship between short sleep duration and obesity among koreans

RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways

Construction organoid model of ovarian endometriosis and the function of estrogen and progesterone in the model

Abstract Endometriosis is a refractory estrogen-dependent gynecological disease in which ovarian endometriosis(OE) is the most common, and the main cell components are endometrial epithelial cells and stromal cells. However, constructing ectopic endometrial epithelial cell models in basic studies is still challenging. In this study, we explored the feasibility and influencing factors of constructing and validating eutopic and ectopic endometrial organoid models of OE as in-vitro models. Eutopic and ectopic endometrial tissues of OE patients were selected to establish organoids. Morphologically, the organoids showed a three-dimensional glandular structure with vacuoles or cystic irregularities, and the histological features of the epithelial organoids in endometriosis were well preserved. Immunofluorescence showed positive expression of epithelial markers and estrogen/progesterone receptors. Genetic identification revealed a 100% match between endometriosis epithelial organoids and endometrial tissue, indicating a common origin. The effects of estrogen and progesterone on the proliferation and secretion of organoids differed with the change in concentration. The successful construction of ectopic endometrial organoids provides a new in vitro model for drug intervention and mechanism study of ovarian endometriosis.

Local chemical inhomogeneity enables superior strength-ductility-superelasticity synergy in additively manufactured NiTi shape memory alloys

Neural mechanisms underlying strain preference behaviour and plasticity in mice

Creation of a eukaryotic multiplexed site-specific inversion system and its application for metabolic engineering

Memorability of novel words correlates with anterior fusiform activity during reading

Preclinical tumor control with a laser-accelerated high-energy electron radiotherapy prototype

A tunable entangled photon-pair source based on a Van der Waals insulator

Droplet-supported liquid-liquid lateral phase separation as a step to floating protein heterostructures

Dual-mode action of scalable, high-quality engineered stem cell-derived SIRPα-extracellular vesicles for treating acute liver failure

A concise enzyme cascade enables the manufacture of natural and halogenated protoberberine alkaloids

Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development

Electrochemical epoxidation enhanced by C2H4 activation and hydroxyl generation at the Ag/SnO2 interface

Redox profiling of preovulatory follicular fluid in the donkey is species-specific, and contributes to modulate sperm function

Coupling of electronic transition to ferroelectric order in a 2D semiconductor

Vascular access dysfunction incidence among Japanese dialysis patients from NDB Open Data Japan