Zonated mechanosensing by PIEZO1 controls liver regeneration

Y Ying Zhang Y Yuzhuo Sun (Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) G Guangkui Xu (Department of Engineering Mechanics, State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University, Xi’an, China.) Y Yue Wu (Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.) Z Zhijun Shi (Department of Engineering Mechanics, State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University, Xi’an, China.) Z Zhuo Chang (Department of Engineering Mechanics, State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University, Xi’an, China.) J Junjun Liu (Techshake Biotechnology Co., Ltd., Xi’an, Shaanxi, China.) K Kaidan Pang (Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) S Siyu Liu H Han Wang W Weixin Rong (Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) Z Zehua Shao (Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) X Xingqian Liu (Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) Y Yu He (Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) Y Yang Yan (Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) B Bin Zhou Z Zuyi Yuan (Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) X Xu-Feng Zhang (Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) S Stefan Offermanns (Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.) S Shengpeng Wang (Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.)

Abstract

The liver exhibits a marked regenerative capacity organized through distinct zones, yet how tissue mechanics coordinate zonated proliferation remains elusive. We reveal that mechanical cues critically contribute to mouse liver regeneration in a highly region-specific manner through sensing by a subpopulation of mid-lobular hepatocytes, which are characterized by dipeptidyl peptidase-4 (DPP4) expression and represent the key proliferative pool of hepatocytes. PIEZO1 is a primary mechanosensor enriched in zone 2 DPP4 + hepatocytes that integrates biomechanical cues to drive liver regrowth by insulin-like growth factor binding protein 2 (IGFBP2). Genetic disruption of PIEZO1 restrains hepatocyte proliferation and compromises liver regeneration, whereas zonated PIEZO1 gain of function enhances proliferation and accelerates recovery. These findings reveal that DPP4 + mechanosensitive hepatocytes orchestrate liver regrowth through PIEZO1-mediated mechanosensing, establishing a link between tissue mechanics and liver regeneration.

Article Details

Journal Science
Volume / Issue Vol. 393, Issue 6806
Published July 02, 2026
ISSN 0036-8075
Publisher American Association for the Advancement of Science

Journal Info

Science

American Association for the Advancement of Science

ISSN: 0036-8075 Social Sciences

Authors (20)

Y

Ying Zhang

Y

Yuzhuo Sun

Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

G

Guangkui Xu

Department of Engineering Mechanics, State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University, Xi’an, China.

Y

Yue Wu

Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Z

Zhijun Shi

Department of Engineering Mechanics, State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University, Xi’an, China.

Z

Zhuo Chang

Department of Engineering Mechanics, State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi’an Jiaotong University, Xi’an, China.

J

Junjun Liu

Techshake Biotechnology Co., Ltd., Xi’an, Shaanxi, China.

K

Kaidan Pang

Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

S

Siyu Liu

H

Han Wang

W

Weixin Rong

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

Z

Zehua Shao

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

X

Xingqian Liu

Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

Y

Yu He

Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

Y

Yang Yan

Department of Cardiovascular Surgery, Med-X Institute, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

B

Bin Zhou

Z

Zuyi Yuan

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

X

Xu-Feng Zhang

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

S

Stefan Offermanns

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.

S

Shengpeng Wang

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China.