Applied Physics Letters

Chemical-state imaging of a mammalian cell through multi-elemental soft x-ray spectro-ptychography

K Kai Sakurai (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) Y Yoko Takeo (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) S Shunki Takaramoto (The Institute for Solid State Physics, The University of Tokyo) N Noboru Furuya (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) K Kyota Yoshinaga (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) T Takenori Shimamura (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) J Jordan T. O'Neal (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) Y Yu Nakata (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) S Satoru Egawa K Kazuyoshi Yoshimi (The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,) H Haruhiko Ohashi (Japan Synchrotron Radiation Research Institute 2 , 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198,) H Hidekazu Mimura (RIKEN 3 , SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo 679-5198,) Y Yoshihisa Harada (Institute for Solid State Physics) K Keiichi Inoue M Mari Shimura (RIKEN 3 , SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo 679-5198,) T Takashi Kimura

Abstract

Soft x-ray spectro-ptychography is a high-resolution chemical state imaging technique and has significant potential for the analysis of light-element-rich samples such as biological cells. We measured hyperspectral images of a whole mammalian neuronal cell at the nitrogen and oxygen absorption edges with soft x rays using the achromatic ptychography system CARROT developed at SPring-8 BL07LSU. We visualized and classified the intracellular structures based on the difference of chemical states, which are difficult to recognize in a monochromatic image. The method is expected to give insight into the relationship between intracellular structures and chemical states.

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Article Details

Volume / Issue Vol. 126, Issue 4
Published January 27, 2025
ISSN 0003-6951
Publisher American Institute of Physics

Journal Info

Applied Physics Letters

American Institute of Physics
ISSN: 0003-6951 Physical Sciences

Authors (16)

K

Kai Sakurai

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

Y

Yoko Takeo

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

S

Shunki Takaramoto

The Institute for Solid State Physics, The University of Tokyo

N

Noboru Furuya

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

K

Kyota Yoshinaga

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

T

Takenori Shimamura

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

J

Jordan T. O'Neal

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

Y

Yu Nakata

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

S

Satoru Egawa

K

Kazuyoshi Yoshimi

The Institute for Solid State Physics, The University of Tokyo 1 , 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581,

H

Haruhiko Ohashi

Japan Synchrotron Radiation Research Institute 2 , 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198,

H

Hidekazu Mimura

RIKEN 3 , SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo 679-5198,

Y

Yoshihisa Harada

Institute for Solid State Physics

K

Keiichi Inoue

M

Mari Shimura

RIKEN 3 , SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo 679-5198,

T

Takashi Kimura

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