AFM cantilever magnetometry for measuring Femto-Nm torques generated by single magnetic particles for cell actuation

Abstract

Particles with high anisotropy in their magnetic properties and shape are of increasing interest for mechanobiology, where transducing a remotely applied magnetic field vector to a local mechanical response is crucial. An outstanding challenge is quantifying the mechanical torque of a single nanoparticle, typically in the range of atto- to femto-Newton-meters (Nm). The magneto-mechanical torque manifests due to a misalignment of the external magnetic field vector with the built-in magnetic anisotropy axis, as opposed to a magnetic force, and complicates the measurement scheme. In this work, we developed a method using a commercially available atomic force microscopy setup and cantilevers to quantify the torque generated by a single synthetic antiferromagnetic (SAF) nanoplatelet with high perpendicular magnetic anisotropy. Specifically, we measured 1.6 ± 0.6 × 10−15 Nm torque while applying 373 ± 5 mT field at 12 ± 2° angle to the built-in anisotropy axis exerted by a single circular SAF nanoplatelet with 1.88 μm diameter and 72 nm thickness, naively translating to a ≈1.7 nN maximum force at the nanoplatelet apex. This measured torque and derived force of the SAF nanoplatelets is strong enough for most applications in mechanobiology; for example, it can be used to rupture (cancer) cell membranes. Moreover, SAF nanoplatelets open a route for easy tuning of the built-in magnetic anisotropy and size, reducing the torque and allowing for small mechanical stimuli for ion channel activation. This work presents a straightforward and widely applicable method for characterizing magnetic particles' mechanical transduction, which is applied to SAF nanoplatelets with a high perpendicular magnetic anisotropy.