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Molecular and biological characterization of infectious full-length cDNA clones of two viruses in Paris yunnanensis, including a novel potyvirus
Biological functions of extracellular vesicle double C2-like domain beta in cervical cancer
Abstract Double C-2 Like Domain Beta (DOC2B) located at 17q13.3 prevents metastasis by senescence induction and epithelial to mesenchymal transition inhibition in cervical cancer (CC). The extracellular vesicle (EV) mediated trafficking of DOC2B and its impact on tumor suppressive activity are not investigated in CC. Using a retroviral method, we first ectopically expressed DOC2B in SiHa, which do not normally express DOC2B. DOC2B-SiHa and vector-SiHa EVs were co-incubated separately with recipient cell and subjected to various cellular and biochemical experiments. For the first time, we demonstrated that DOC2B localizes to EVs, and its transfer to EV may require intracellular calcium. Co-culture of SiHa and HeLa cells with DOC2B-SiHa derived EVs induced morphological changes and suppressed their growth and migration, possibly by induction of G0/G1 to S phase arrest and anoikis. DOC2B-SiHa EVs elevated intracellular reactive oxygen species (ROS) and calcium levels and promoted lipid droplet accumulation and lipid peroxidation rate in recipient cells. DOC2B-SiHa EVs reduced active AKT1 and ERK1/2 levels and EMT marker expression and enhanced cellular senescence and cytotoxic effects of cisplatin. Re-expression of DOC2B significantly altered the global metabolite profile of EVs. Finally, we demonstrated that intracellular calcium chelation significantly reduces DOC2B localization to EVs and impacts its tumor-suppressive properties. Altogether, EV-mediated DOC2B transfer may reduce the aggressive behavior of CC cells.
Short-term warming supports mineral-associated carbon accrual in abandoned croplands
A radiological study on the relationship between the novel acromial angle and rotator cuff tears
Sounds like gambling: detection of gambling venue visitation from sounds in gamblers’ environments using a transformer
A deep multiple instance learning framework improves microsatellite instability detection from tumor next generation sequencing
Prevalence and risk factors of long COVID-19 persisting for 2 years in Hainan Province: a population-based prospective study
Effect of health literacy-based teach-back training on quality of life and treatment adherence in type 2 diabetes: an experimental study
A flexible catheter-based sensor array for upper airway soft tissues pressure monitoring
AbstractObstructive sleep apnea is a globally prevalent concern with significant health impacts, especially when coupled with comorbidities. Accurate detection and localization of airway obstructions are crucial for effective diagnosis and treatment, which remains a challenge for traditional sleep monitoring methods. Here, we report a catheter-based flexible pressure sensor array that continuously monitors soft tissue pressure in the upper airway and facilitates at the millimeter level. The sensor’s design and versatile 3D femtosecond laser fabrication process enable adaptation to diverse materials and applications. In vitro testing demonstrates high sensitivity (38.1 Ω/mmHg) and excellent stability. The sensor array effectively monitors distributed airway pressure and accurately identifies obstructions in an obstructive sleep apnea animal model. In this work, we highlight the potential of this catheter-based sensor array for long-term, continuous upper airway pressure monitoring and its prospective applications in other medical devices for pressure measurement in human body cavities.
MRI imaging characteristics of brainstem and midbrain aqueduct in patients with iNPH
Measurements on slope parameter mapping based on dual-energy CT enterography for improving Crohn’s disease diagnosis and inflammatory activity evaluation
Deciphering metabolic differentiation during Bacillus subtilis sporulation
AbstractThe bacterium Bacillus subtilis undergoes asymmetric cell division during sporulation, producing a mother cell and a smaller forespore connected by the SpoIIQ-SpoIIIA (or Q-A) channel. The two cells differentiate metabolically, and the forespore becomes dependent on the mother cell for essential building blocks. Here, we investigate the metabolic interactions between mother cell and forespore using genome-scale metabolic and expression models as well as experiments. Our results indicate that nucleotides are synthesized in the mother cell and transported in the form of nucleoside di- or tri-phosphates to the forespore via the Q-A channel. However, if the Q-A channel is inactivated later in sporulation, then glycolytic enzymes can form an ATP and NADH shuttle, providing the forespore with energy and reducing power. Our integrated in silico and in vivo approach sheds light into the intricate metabolic interactions underlying cell differentiation in B. subtilis, and provides a foundation for future studies of metabolic differentiation.
Discovering natural products as potential inhibitors of SARS-CoV-2 spike proteins
The coherent structure of the energy cascade in isotropic turbulence
π-PrimeNovo: an accurate and efficient non-autoregressive deep learning model for de novo peptide sequencing
Type 2 diabetes prediction method based on dual-teacher knowledge distillation and feature enhancement
Heart-retina time analysis using electrocardiogram-coupled time-resolved dynamic optical coherence tomography
AbstractThe eye and the heart are two closely interlinked organs, and many diseases affecting the cardiovascular system manifest in the eye. To contribute to the understanding of blood flow propagation towards the retina, we developed a method to acquire electrocardiogram (ECG) coupled time-resolved dynamic optical coherence tomography (OCT) images. This method allows for continuous synchronised monitoring of the cardiac cycle and retinal blood flow dynamics. The dynamic OCT measurements were used to calculate time-resolved blood flow profiles using fringe washout analysis. The relative fringe washout was computed to generate the flow velocity profiles within arterioles at the optic nerve head rim. We found that the blood column between the heart and the retina propagates within one cardiac cycle, denoting the arrival time as the heart-retina time (HRT). In a group of healthy subjects, the HRT was 144 ± 19 ms (mean ± SD). The HRT could provide a novel potential biomarker for cardiovascular health in direct relation to retinal perfusion.