Browse Articles

Fibrosis and inflammatory activity diagnosis of chronic hepatitis C based on extreme learning machine

Single-cell RNA sequencing identifies CXADR as a fate determinant of the placental exchange surface

AbstractThe placenta is the critical interface between mother and fetus, and consequently, placental dysfunction underlies many pregnancy complications. Placental formation requires an adequate expansion of trophoblast stem and progenitor cells followed by finely tuned lineage specification events. Here, using single-cell RNA sequencing of mouse trophoblast stem cells during the earliest phases of differentiation, we identify gatekeepers of the stem cell state, notably Nicol1, and uncover unsuspected trajectories of cell lineage diversification as well as regulators of lineage entry points. We show that junctional zone precursors and precursors of one of the two syncytial layers of the mouse placental labyrinth, the Syncytiotrophoblast-I lineage, initially share similar trajectories. Importantly, our functional analysis of one such lineage precursor marker, CXADR, demonstrates that this cell surface protein regulates the differentiation dynamics between the two syncytial layers of the mouse labyrinth, ensuring the correct establishment of the placental exchange surface. Deciphering the mechanisms underlying trophoblast lineage specification will inform our understanding of human pregnancy in health and disease.

Genetic analysis and association detection of agronomic traits in maize genotypes

Amelioration of the growth and physiological responses of Capsicum annum L. via quantum dot-graphene oxide, cerium oxide, and titanium oxide nanoparticles foliar application under salinity stress

A Y chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae

Abstract Genetic control – the deliberate introduction of genetic traits to control a pest or vector population – offers a powerful tool to augment conventional mosquito control tools that have been successful in reducing malaria burden but that are compromised by a range of operational challenges. Self-sustaining genetic control strategies have shown great potential in laboratory settings, but hesitancy due to their invasive and persistent nature may delay their implementation. Here, instead, we describe a self-limiting strategy, designed to have geographically and temporally restricted effect, based on a Y chromosome-linked genome editor (YLE). The YLE comprises a CRISPR-Cas9 construct that is always inherited by males yet generates an autosomal dominant mutation that is transmitted to over 90% of the offspring and results in female-specific sterility. To our knowledge, our system represents a pioneering approach in the engineering of the Y chromosome to generate a genetic control strain for mosquitoes. Mathematical modelling shows that this YLE technology is up to seven times more efficient for population suppression than optimal versions of other self-limiting strategies, such as the widely used Sterile Insect Technique or the Release of Insects carrying a Dominant Lethal gene.

Fascicle differentiation of upper extremity nerves on high-resolution ultrasound with multimodal microscopic verification

AbstractThis study aimed to compare the fascicular anatomy of upper limb nerves visualized using in situ high-resolution ultrasound (HRUS) with ex vivo imaging modalities, namely, magnetic resonance microscopy (MRM), histological cross-sections (HCS), and optical projection tomography (OPT). The median, ulnar, and superficial branch of radial nerve (n = 41) were visualized in 14 cadaveric upper limbs using 22-MHz HRUS. Subsequently, the nerves were excised, imaged with different microscopic techniques, and their morphometric properties were compared. HRUS accurately differentiated 51–74% of fascicles, while MRM detected 87–92% of fascicles when compared to the referential HCS. Among the compared modalities, HRUS demonstrated the smallest fascicular ratios and fascicular cross-sectional areas, but the largest nerve cross-sectional areas. The probability of a fascicle depicted on HRUS representing a cluster of multiple fascicles on the referential HCS increased with the fascicular size, with some differences observed between the larger median and ulnar nerves and the smaller radial nerves. Accordingly, HRUS fascicle differentiation necessitates cautious interpretation, as larger fascicles are more likely to represent clusters. Although HCS is considered the reference modality, alterations in nerve cross-sectional areas or roundness during sample processing should be acknowledged.

In silico analysis of human herpes virus-8 genome: a comparison of the K1, VR1, and VR2 regions for genotyping and global geographical distribution

Tuning the selectivity of NH3 oxidation via cooperative electronic interactions between platinum and copper sites

AbstractSelective catalytic oxidation (SCO) of NH3 to N2 is one of the most effective methods used to eliminate NH3 emissions. However, achieving high conversion over a wide operating temperature range while avoiding over-oxidation to NOx remains a significant challenge. Here, we report a bi-metallic surficial catalyst (PtSCuO/Al2O3) with improved Pt atom efficiency that overcomes the limitations of current catalysts. It achieves full NH3 conversion at 250 °C with a weight hourly space velocity of 600 ml NH3·h−1·g−1, which is 50 °C lower than commercial Pt/Al2O3, and maintains high N2 selectivity through a wide temperature window. Operando XAFS studies reveal that the surface Pt atoms in PtSCuO/Al2O3 enhance the redox properties of the Cu species, thus accelerating the Cu2+ reduction rate and improving the rate of the NH3-SCO reaction. Moreover, a synergistic effect between Pt and Cu sites in PtSCuO/Al2O3 contributes to the high selectivity by facilitating internal selective catalytic reduction.

A bi-level optimization strategy of electricity-hydrogen-carbon integrated energy system considering photovoltaic and wind power uncertainty and demand response

Psychometric properties of the Arabic version of the Young Schema Questionnaire Short Form-Version 3 (YSQ-S3)

Enhanced motivated behavior mediated by pharmacological targeting of the FGF14/Nav1.6 complex in nucleus accumbens neurons

Association between lifestyle risk factors and mortality in the Mexico City prospective study

Determinants of outcomes in patients with hepatitis B virus-decompensated cirrhosis

Synaptic signatures and disease vulnerabilities of layer 5 pyramidal neurons

FSTL1 aggravates high glucose-induced oxidative stress and transdifferentiation in HK-2 cells

Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy

Quantum key distribution implemented with d-level time-bin entangled photons

AbstractHigh-dimensional photon states (qudits) are pivotal to enhance the information capacity, noise robustness, and data rates of quantum communications. Time-bin entangled qudits are promising candidates for implementing high-dimensional quantum communications over optical fiber networks with processing rates approaching those of classical telecommunications. However, their use is hindered by phase instability, timing inaccuracy, and low scalability of interferometric schemes needed for time-bin processing. As well, increasing the number of time bins per photon state typically requires decreasing the repetition rate of the system, affecting in turn the effective qudit rates. Here, we demonstrate a fiber-pigtailed, integrated photonic platform enabling the generation and processing of picosecond-spaced time-bin entangled qudits in the telecommunication C band via an on-chip interferometry system. We experimentally demonstrate the Bennett-Brassard-Mermin 1992 quantum key distribution protocol with time-bin entangled qudits and extend it over a 60 km-long optical fiber link, by showing dimensionality scaling without sacrificing the repetition rate. Our approach enables the manipulation of time-bin entangled qudits at processing speeds typical of standard telecommunications (10 s of GHz) with high quantum information capacity per single frequency channel, representing an important step towards an efficient implementation of high-data rate quantum communications in standard, multi-user optical fiber networks.

The effect of PEO/NaCl dual porogens in the fabrication of porous PCL membranes via a solid-state blending approach

The presence of pleural effusion is an independent prognostic factor in patients with malignant pleural mesothelioma

A higher order PUF complex is central to regulation of C. elegans germline stem cells

AbstractPUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode PUF proteins maintain germline stem cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including gld-1. Here we report that PUF protein FBF-2 and its partner LST-1 form a ternary complex that represses gld-1 via a pair of adjacent FBF binding elements (FBEs) in its 3′UTR. One LST-1 molecule links two FBF-2 molecules via motifs in the LST-1 intrinsically-disordered region; the gld-1 FBE pair includes a well-established ‘canonical’ FBE and a newly-identified noncanonical FBE. Remarkably, this FBE pair drives both full RNA repression in GSCs and full RNA activation upon differentiation. Discoveries of the LST-1–FBF-2 ternary complex, the gld-1 adjacent FBEs, and their in vivo significance predict an expanded regulatory repertoire of different assemblies of PUF-partner-RNA higher order complexes in nematode GSCs. This also suggests analogous PUF controls may await discovery in other biological contexts and organisms.