Browse Articles

Numerical modeling of oxygen diffusion in tissue spheroids undergoing fusion using function representation and finite volumes

Abstract A three-dimensional cell culture called a spheroid serves as a foundational entity in a wide variety of modern tissue engineering applications, including 3D-bioprinting and preclinical drug testing. Lack of oxygen within tissue spheroids hinders metabolism of cells and eventually leads to cell death. Prevention of necrosis is crucial to success of tissue engineering methods and such prevention requires estimation of cell viability in the spheroid. We propose a novel approach for numerical modeling of diffusion in tissue spheroids during their fusion. The approach is based on numerical solutions of partial differential equations and the application of Function Representation (FRep) framework for geometric modeling. We present modeling of oxygen diffusion based on meshes derived from the geometry of fusing spheroids, a method for selecting optimal spheroid size, and several statistics for estimating cellular viability. Our findings provide insights into oxygen diffusion in three-dimensional cell cultures thus improving the robustness of biotechnological methods that employ tissue spheroids.

Estimating the legibility of international borders

Researchers in the social sciences are interested in the consequences of institutions, increasingly on a global scale. Institutions that may be negotiated between states can have consequences at a microlevel, as local populations adjust their expectations and ultimately even their behavior to take institutional rules into account. However, large-scale fine-grained analyses that test for the complex evidence of such institutions locally are rare. This article focuses on a key institution: International borders. Using computer vision techniques, we show that it is possible to produce a geographically specific, validated, and replicable way to characterize border legibility , by which we mean the ability to visually detect the presence of an international border in physical space. We develop and compare computer vision techniques to automatically estimate legibility scores for 627,656 imagery tiles from virtually every border in the world. We evaluate statistical and data-driven computer vision methods, finding that fine-tuning pretrained visual recognition models on a small set of human judgments allows us to produce local legibility scores globally that align well with human notions of legibility. Finally, we interpret these scores as useful approximations of states’ border orientations, a concept that prior literature has used to capture the visible investments states make in border areas to maintain jurisdictional authority territorially. We validate our measurement strategy using both human judgments and five nomological validation indicators.

Privacy-preserving method for face recognition based on homomorphic encryption

In recent years, facial recognition technology has been widely adopted in modern society. However, the plaintext storage, computation, and transmission of facial data have posed significant risks of information leakage. To address this issue, this paper proposes a facial recognition framework based on approximate homomorphic encryption (HE_FaceNet), aimed at effectively mitigating privacy leaks during the facial recognition process. The framework first utilizes a pre-trained model to extract facial feature templates, which are then encrypted. The encrypted templates are matched using Euclidean distance, with the final recognition being performed after decryption. However, the time-consuming nature of homomorphic encryption calculations limits the practical applicability of the HE_FaceNet framework. To overcome this limitation, this paper introduces an optimization scheme based on clustering algorithms to accelerate the facial recognition process within the HE_FaceNet framework. By grouping similar faces into clusters through clustering analysis, the efficiency of searching encrypted feature values is significantly improved. Performance analysis indicates that the HE_FaceNet framework successfully protects facial data privacy while maintaining high recognition accuracy, and the optimization scheme demonstrates high accuracy and significant computational efficiency across facial datasets of varying sizes.

Artificial intelligence models development for profitability factor prediction in concentrated solar power with dual backup systems

Evidence for domain-general arousal from semantic and neuroimaging meta-analyses reconciles opposing views on arousal

Arousal refers to changes in brain-body state underpinning motivated behavior but lacks a proper definition and taxonomy. Neuroscience and psychology textbooks offer surprisingly different views on what arousal is, from a global brain-wide modulation of neuronal activity to a multidimensional construct, with specific brain-body patterns tuned to a given situation. The huge number of scientific articles mentioning arousal (~50,000) highlights the importance of the concept but also explains why such a vast literature has never been systematically reviewed so far. Here, we leverage the tools of natural language processing to probe the nature of arousal in a data-driven, comprehensive manner. We show that arousal comes in seven varieties: cognitive, emotional, physiological, sexual, related to stress disorders, to sleep, or to sleep disorders. We then ask whether domain-general arousal exists at the cortical level, and run meta-analyses of the brain imaging literature to reveal that all varieties of arousal, except arousal in sleep disorders for lack of data, converge onto a cortical network composed of the presupplementary motor area and the left and right dorsal anterior insula. More precisely, we find that activity in dysgranular insular area 7 (Jülich atlas), the region with the highest convergence across varieties of arousal, is also specifically associated with arousal. The domain-general arousal network might trigger the reorganization of large-scale brain networks—a global mechanism—resulting in a context-specific configuration—in line with the multidimensional view. Future taxonomies of arousal refining the alignment between concepts and data should include domain-general arousal as a central component.

Understanding the history of the late Roman synagogue at Huqoq in Israel’s Galilee through radiocarbon dating and observations on site formation

Galilean-type synagogues are monumental, basilical structures found in northern Israel that have long been dated, mainly on stylistic grounds, to the 2nd – 3rd centuries CE. This chronology is influenced by historical considerations – specifically, the notion that monumental synagogues must have been constructed before Jews came under Christian rule in the early 4th century. However, the stratigraphic contexts of the pottery and coins associated with the foundation of these buildings suggest they represent an architectural innovation dating to the 4th (especially the later 4th) to 6th centuries CE. The Huqoq Excavation Project was initiated in 2011 with the goal of determining the construction date of a Galilean-type synagogue through controlled stratigraphic excavation, including the systematic collection of samples for radiocarbon dating along with micromorphological analysis of the fill deposits. Here we present the initial results of these analyses, which indicate that the radiocarbon ages conform with the pottery data to place the construction of the Huqoq synagogue in the late 4th – early 5th centuries (late Roman period). However, the ages of dated samples from thick fills overlying the floor, which were deposited when the synagogue was rebuilt and expanded in the early 14th century (late medieval/Mamluk period) do not reflect the stratigraphic sequence.

Economic renovation of a hillside drip irrigation system using drip tape inlet pressure regulators under computer-aided design

Simulating atmospheric freezing of single aqueous droplets to ice in a cryogenically cooled ultrasonic levitator

Atmospheric freezing of water droplets suspended in air followed by cloud formation and precipitation represent fundamental steps of the terrestrial water cycle. These aqueous droplets exhibit distinct freezing mechanisms and thermodynamic requirements compared to bulk water often forming metastable supercooled water at subzero temperatures on the Celsius scale (<273 K) prior to crystallization. Here, we report on a real-time spectroscopic investigation combined with simultaneous visualizations of single aqueous droplet freezing events inside a cryogenically cooled ultrasonic levitation chamber with the ultimate goal of probing the molecular structure evolution and stages of ice formation. The observed droplet freezing follows a pseudoheterogeneous ice nucleation mechanism mimicking the process that occurs for atmospherically supercooled water droplets at the air–water interface. This proof-of-concept experimental setup allows future crystallization studies of homo- and heterogeneously doped aqueous droplets under simulated atmospheric environments—also in the presence of reactive trace gases, thus untangling dynamic molecular interactions and chemical reactions, which are of fundamental interest to low-temperature atmospheric chemistry delineating with ice nucleation mechanisms.

A Study on the psychometric properties of the Chinese version of the Parent Tic Questionnaire

Introduction The Parent Tic Questionnaire (PTQ) is a tool for parents to assess their child’s tic severity, but its effectiveness in non-Western contexts like China has not been thoroughly examined. This study aimed to evaluate the psychometric properties of the PTQ in assessing motor and vocal tic severity among Chinese children diagnosed with tic disorders. Method Parents of 268 Chinese children and adolescents aged 6–17 years, diagnosed with tic disorders, completed the PTQ. The study assessed tic severity using the Yale Global Tic Severity Scale (YGTSS) and PTQ. Additionally, obsessive-compulsive symptoms were measured using the Children Yale-Brown Obsessive Compulsive Scale (CY-BOCS), and other behavioral problems were evaluated using the Conners’ Parent Rating Scale (CPRS-48). Results The PTQ showed acceptable to good internal consistency (Cronbach’s alpha = 0.67 for motor, 0.77 for vocal, and 0.79 for total tic scores) and acceptable to good two-week test-retest reliability (intraclass correlations, ICC = 0.74 for motor, 0.81 for vocal, and 0.79 for total tic scores). It demonstrated good convergent validity with the YGTSS and effective discriminant validity from obsessive-compulsive and internalizing symptoms (i.e., anxiety and psychosomatic). Furthermore, our analysis revealed significant variability in item difficulty across the questionnaire, indicating differences in how various tics are perceived and reported by Chinese parents, which may influence the assessment’s accuracy and reliability. Discussion The findings indicate that the Chinese version of the PTQ is a reliable and valid tool for assessing tic severity in Chinese children with tic disorders, offering significant implications for clinical assessment in diverse cultural contexts. Additionally, our findings on item difficulty highlight the need for further cultural adaptations of the PTQ.

Food classifications provide an approximate packaging indicator to support monitoring of mismanaged plastic waste

Abstract Mismanaged plastic waste undermines environmental quality, aquatic ecosystems and ultimately public health. Mismanaged plastics increasingly originate from urban populations lacking waste collection services. Household expenditure survey analyses have quantified plastic waste generation among these populations, but only for individual products (e.g. bagged or bottled drinking-water), not for the overall packaging profile of households’ basket-of-goods. This study aims to evaluate how far the international NOVA classification of foods/beverages and commodity classifications by residents predict plastic food packaging. Via a cross-sectional market surveillance survey, packaging was observed for 502 and 396 transactions at selected retail outlets serving low-income areas of Greater Accra, Ghana, and Kisumu, Kenya respectively. In both cities, NOVA processed/ultra-processed food and food/beverage classes created by local residents were significantly associated with greater plastic food packaging, with similar predictive performance. Plastic packaging prevalence was also significantly higher in supermarkets. Plastic packaging use was significantly lower in Kisumu, where single-use carrier bags are banned. Thus, given their international availability and national representativeness, household expenditure surveys have potential for monitoring to inform policy by targeting plastic pollution hot-spots. However, survey-based monitoring should incorporate expert knowledge of national context and the retail environment to reflect the realities of packaging use.

Broad-spectrum tolerance to disinfectant-mediated bacterial killing due to mutation of the PheS aminoacyl tRNA synthetase

Disinfectants are essential tools for controlling infectious diseases and maintaining sterile conditions in many medical and food-industry settings. Recent work revealed that a deficiency in the carbohydrate phosphotransferase system (PTS) confers pan-tolerance to killing by diverse disinfectant types through its interaction with the cAMP-CRP regulatory network. The present work characterized a pan-tolerance mutant obtained by enrichment using phenol as a lethal probe and an Escherichia coli PTS null mutant as a parental strain. The resulting super-pan-tolerant mutant, which harbored an F158C substitution in PheS, inhibited bacterial killing by multiple disinfectant classes with surprisingly little effect on antimicrobial lethality. The PheS substitution, which was expected to lower substrate recognition efficiency and result in deacylated tRNA phe occupying the ribosomal A site, activated relA expression and synthesis of ppGpp, even in the absence of disinfectant exposure. ppGpp, along with DksA, increased RpoS function by activating promoters of dsrA and iraP , two genes whose products increase the expression and stability of RpoS. Subsequently, RpoS upregulated the expression of genes encoding a universal stress protein (UspB) and an oxidative stress peroxidase (KatE), which preconditioned bacteria to better survive a variety of disinfectants. Disinfectant-mediated accumulation of reactive oxygen species (ROS) and bacterial killing were abolished/reduced by exogenous dimethyl sulfoxide and by a PheS F158C substitution up-regulating genes encoding ROS-detoxifying enzymes ( katE, sodA, oxyR, ahpC ). These data identify a pheS mutation-triggered, ppGpp-stimulated transcriptional regulatory cascade that negates biocide-mediated lethality, thereby tying the stringent response to protection from ROS-mediated biocide lethality.

Correlation between baseline anion gap and early acute kidney injury in patients with acute pancreatitis in the intensive care unit: A single-center retrospective cohort study

Objective Acute pancreatitis (AP) is an acute inflammatory disease that can lead to multiple system dysfunction, including acute kidney injury (AKI). AKI occurs in 10%-42% of AP patients, and studies have shown that early (48 hours) acute pancreatitis associated acute kidney injury (AP-AKI) can increases the risk of death in acute pancreatitis. Anion gap (AG) is a common index in clinical evaluation of acid-base imbalance and an important index in critically ill patients. The aim of this study was to investigate the relationship between baseline anion gap values and early acute kidney injury in patients with acute pancreatitis in intensive care unit. Methods Our data were derived from inpatients admitted to Beth Israel Deaconess Medical Center (BIDMC) in the United States between 2008 and 2019. A total of 4,017 adult patients with acute pancreatitis admitted to the ICU were enrolled in the study, and 475 were enrolled according to the exclusion and inclusion criteria. Only the baseline value and one day after arrival to the intensive care unit (ICU) were considered for all laboratory test values. According to previous literature and clinical significance, AG was divided into two groups: low value (< 16mmol/L) group and high value (≥16mmol/L) group, and logistics univariate and multifactor regression analysis was applied to verify the relationship between anion gap and AKI risk. Results Only 157 of the 475 AP cases had an AG level below 16 mmol/L, whereas 318 patients had an AG level over 16 mmol/L. Within 48 hours, 89 and 240 cases (56.7% and 75.5%) and the low- and high-AG groups had AKI. In AP cases, an elevated AG was related to an increased risk of AKI [odds ratio (OR) = 1.06, 95% confidence interval (CI): 1.03–1.1], and is a nonlinear relationship. When controlling for other factors, this correlation was still significant. Compared to the lower group, high-AG (≥16mmol/L) values can increase the risk of early acute kidney injury in patients with acute pancreatitis (OR = 2.35, CI: 1.57–3.53). Conclusion Anion gap (AG) is an independent risk factor for early acute kidney injury in patients with acute pancreatitis, and has a nonlinear relationship with 48-hour AKI. Higher AG(≥16mmol/L) values can significantly increase the risk of AP-AKI.

Assessment of compressive strength of eco-concrete reinforced using machine learning tools

Association between glycosylated hemoglobin and blood lead: A cross-sectional study

Background Diabetes is the most common chronic metabolic disease, affecting many people's health. Previous studies have shown a close relationship between trace elements and metabolic diseases. This study investigated the interrelationship between glycosylated hemoglobin (HbA1c) and blood lead (BPb) in adults. Method This research was carried out involving 12,049 eligible individuals aged 20 years or above from the National Health and Nutrition Examination Survey (NHANES) spanning from 2011 to 2020. Weighted linear regression models and smoothed curve fitting were employed to investigate the association between HbA1c and blood lead. Analyses were stratified based on age, sex, race, and body mass index, and threshold effects were explored using two-stage segmented linear regression models. Result Among all 12049 participants, through comprehensive adjustment of the model, this study discovered a negative association between HbA1c and blood lead. In addition, when stratified by sex, age, race, and BMI status in subgroup analysis in this study, this correlation still had specific statistical significance. In performing subgroup analyses, we found that the relationship between HbA1c and blood lead may yield distinct outcomes arise from gender disparities. In women, a significant U-shaped association exists between HbA1c and BPb. At approximately 6.6% of HbA1c value, the relationship between the two shifts from negative to positive. Conclusion This investigation proposes a “U” form association between HbA1c and BPb in American adults.

Oxygen and hydrobiological profiles of homemade manure-based tea in North Africa

Abstract Homemade manure tea (HMT) is commonly used in North Africa to enhance crop yields. Yet their physicochemical and biological characteristics remain poorly understood. This study evaluated oxygen and hydrobiological profiles of three types of HMT (bovine, ovine and poultry based, respectively noted HMTb, HMTo, HMTp) and compared them to control solutions of water and water supplemented with soluble NPK fertilizer. For these three types of HMT, oxygen and hydrobiological profiles were measured daily over a 7-day incubation period in three repeated, identical experiments, each comprising randomized treatments and five repetitions per treatment. Our results show that all HMT types rapidly transitioned to hypoxic conditions in the first 24h, shifting to anoxia between day 2 and day 7 depending on HMT type. This anoxic environment promoted denitrification and led to elevated NH4 + concentrations, suggesting the presence of anammox and microaerobic processes. Particulate organic matter contents and bacterial densities were highest in HMTp, while ciliate densities were highest in HMTb. These findings underscore the bioactive potential of HMT as fertilizers, with HMTp showing a favorable nitrogen profile beneficial for agricultural applications. To maintain aerobic conditions longer and reduce nitrogen losses and greenhouse gas emissions, we recommend passive or mechanical aeration, applying HMT during cooler hours, and stabilizing the pH of HMT. This study offers valuable insights to refine HMT preparation protocols, enhancing their use as bioactive fertilizers.

Network study of miRNA regulating traumatic heterotopic ossification

Objective Objective: To identify and analyze the microRNAs that are expressed differently (DE-miRNAs) and forecast their potential roles in the pathophysiological process of traumatic heterotopic ossification (THO). Methods We conducted RNA sequencing on six samples of normal bone and THO tissues from the patients and conducted differential expression analysis of miRNA. The biological activities of the target genes of the differentially expressed microRNAs (DE-miRNAs) were investigated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The miRNA-mRNA network was constructed using Cytoscape software, incorporating miRNAs with varying expression levels and their corresponding target genes. Results In comparison to the normal control group, a total of 84 differentially expressed microRNAs (p<0.05, |log2FC|>1) were identified, with 27 microRNAs showing up-regulation and 57 microRNAs showing down-regulation. The functional enrichment analysis revealed that the target genes of the de-mirna were primarily enriched in biological processes such as the regulation of protein stability and the management of neuromuscular process balance. Additionally, a miRNA-mRNA expression regulatory network was established. The RT-qPCR analysis revealed that miR-142-3p, miR-150-5p, miR-421, miR-625-5p, miR-675-5p, and miR-940 exhibited a decrease in expression levels in THO tissues. Nevertheless, the expression levels of miR-181c-3p, miR-320c, miR-497-5p, and miR-99a-5p were increased in THO tissues. Conclusions Our investigation has uncovered the expression patterns and projected the potential activities of differentially expressed microRNAs (DE-miRNAs) in human THO. This research may contribute to a better understanding of the underlying mechanisms and offer new possibilities for therapeutic targets in THO.

Agromorphological and biochemical characterization of mutagens induced M3 mutant lines of grasspea

WDR75: An essential protein for ribosome assembly undergoing purifying selection

Ribosomes, vital for life, consist of a large subunit and a small subunit (SSU), the latter is crucial for translation initiation and mRNA binding. The SSU processome, a 71-protein multimer in humans, is an intermediate in ribosome formation. One of its constituents, WDR75 plays a pivotal role by binding to an evolutionary conserved motif in the external transcribed spacer region of the rRNA to help form the SSU. Herein, we explore mammalian WDR75 molecular evolution, 3D structure, and phylogeny in light of its essential role in the SSU processome. We predict to find the footprint of purifying selection, especially at sites that are essential for proper ribosome assembly. In our comparison of 70 mammalian WDR75 sequences, we found ~25% of sites with significant purifying selection and no evidence of positive selection. Purifying selection was ~5x stronger for sites folding into beta-sheets than those predicted to be coils. Phylogenetic analysis validated expected mammalian relationships and uncovered an unusually long branch leading to mouse-eared bats, exhibiting 18x more substitutions per site than the average mammalian substitution rate. In testing for molecular evolution among branches, we found no evidence for purifying selection along any individual branches, but unexpectedly detected significant diversifying selection solely among African great apes.

A configurational path study of adolescents’ intention to participate in ice and snow sports based on the TPB and NAM frameworks

The association between different insulin resistance indexes and bone health in the elderly

The triglyceride-glucose (TyG) index and its related indexes (TyG-BMI, TyG-WC, TyG-WHtR) are effective markers for screening metabolic diseases like insulin resistance (IR). However, few studies have explored the relationship between the TyG and its related indexes with bone density (BMD), osteopenia, and osteoporosis. This is a cross-sectional study that involved 1,303 adults aged 50 years and above from the National Health and Nutrition Examination Survey 2007–2010, and 2013–2014. In the multivariable-adjusted model, linear regression analysis and logistic regression analysis demonstrated that TyG and its related indexes have a significant positive correlation with BMD and a negative correlation with osteopenia/osteoporosis in the femoral neck, lumbar spine, and total hip region. Trend analysis further confirms these associations (p < 0.05). Restricted cubic spline analysis showed a nonlinear relationship between these indexes with BMD and osteopenia/osteoporosis. Sensitivity analyses further confirmed the robustness of these associations. This study reveals the significant and complex correlation between the TyG and its related indexes with BMD and osteoporosis, indicating the potential link between IR and bone health. The TyG and related indexes offer a new perspective for the diagnosis, prevention, and treatment of osteoporosis.