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Metabonomics reveals the mechanism of stress resistance in Vetiveria zizanioides inoculated with AMF under copper stress
Identification of shared genetic loci for asthma, allergic rhinitis, and pollinosis in East Asians
Publisher Correction: Phylotranscriptomics resolved phylogenetic relationships and divergence time between 20 golden camellia species
Association between the non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio and mortality among hypertension patients
An All-Metal Broadband Low SLL slot array antenna for use in 5G Sub-6 GHz networks
To study the thermodynamic properties of magnetic field on water based CoFe2O4 nanofluids
Polyunsaturated fatty acids may not be helpful for people with osteoarthritis: a two-sample Mendelian randomization analysis
Real-time warning method for sand plugging in offshore fracturing wells
Author Correction: Consistent FFP2-masking as part of reducing viral respiratory infections on medical wards for allogeneic hematopoietic stem cell transplantation
Thermal performance analysis for roofs with heat mitigation strategies in subtropical hot and humid regions
Association between blood eosinophil levels and prognosis in critically ill patients with different heart failure phenotypes
Perspectives of New York State residents on deer management, hunting, and predator reintroduction
A real-world pharmacovigilance analysis of hepatitis B vaccine using the U.S. Vaccine Adverse Event Reporting System (VAERS) database
A prognostic model for lung adenocarcinoma based on cuproptosis and disulfidptosis related genes revealing the key prognostic role of FURIN
Unveiling the role of sex in the metabolism of indoxyl sulfate and apixaban
Author Correction: Single Source Precursor-based Solvothermal Synthesis of Heteroatom-doped Graphene and Its Energy Storage and Conversion Applications
Prevalence and influencing factors of sarcopenia among older adults in rural coastal areas of Ningbo City
Publisher Correction: Description and phylogenetic implications of a de novo mitochondrial genome of Rhinichthys atratulus (Teleostei: Leuciscidae) from Connecticut
Facilitating high throughput collections-based genomics: a comparison of DNA extraction and library building methods
Abstract While DNA barcoding methods are an increasingly important tool in biological conservation, the resource requirements of constructing reference libraries frequently reduce their efficacy. One efficient way of sourcing taxonomically validated DNA for reference libraries is to use museum collections. However, DNA degradation intrinsic to historical museum specimens can, if not addressed in the wet lab, lead to low quality data generation and severely limit scientific output. Several DNA extraction and library build methods that are designed to work with degraded DNA have been developed, although the ability to implement these methods at scale and at low cost has yet to be formally addressed. Here, the performance of widely used DNA extraction and library build methods are compared using museum specimens. We find that while our selected DNA extraction methods do not significantly differ in DNA yield, the Santa Cruz Reaction (SCR) library build method is not only the most effective at retrieving degraded DNA from museum specimens but also easily implemented at high throughput for low cost. Results highlight the importance of lab protocol on data yield. An optimised “sample to sequencing” high-throughput protocol which incorporates SCR is included to allow for easy uptake by the wider scientific community.