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Alterations in fecal microbiota composition and cytokine expression profiles in adolescents with depression: a case-control study
Long-term expansion of basal cells and the novel differentiation methods identify mechanisms for switching Claudin expression in normal epithelia
Abstract Epithelia are tightly connected cellular sheets, that shield our body from the external environment. They are continuously maintained by a pooled population of undifferentiated cells through differentiation. However, the maintenance mechanisms remain incompletely understood due to the difficulty of experimentally observing the differentiation process. To address this issue, we developed a culture method for long-term expansion of primary mammary basal cells with a set of compounds, that includes undifferentiated cells. An effective differentiation method regarding Claudin expression was also developed by simply removing compounds. To verify this differentiation-switching technique, we obtained microarray data comparing each differentiation state. Subsequent cellular analysis confirmed key transcription factors in each state: (1) EGR1 in undifferentiated basal cells is important for suppressing Claudin expression through maintaining the epithelial-mesenchymal transition (EMT) transcription factor TWIST1, (2) ELF3 in differentiated cells is important for actin organization and subsequent Claudin localization at the cell-cell border, that corresponds to the amount of GRHL3, an actin organizer. Their relevance was also observed in tissues and organoids. In summary, we present an effective tool for verifying molecular mechanisms that determine Claudin status in normal basal cell differentiation, that would be beneficial in epithelial cell biology, cancer biology, physiology, and regeneration research.
Implementation of double Feynman gate in high dimensional quantum systems
Assessment of liver involvement in Wilson’s disease with different liver echo patterns based on liver stiffness evaluated on Transient elastography and Sound Touch Viscoelastography
Monocyte-to-HDL cholesterol ratio (MHR) as a novel Indicator of gout risk
A lightweight deep learning model for multi-plant biotic stress classification and detection for sustainable agriculture
Transcriptional regulation of ETV5 by mitogen-activated protein kinase via ETS-1 in human pancreatic cancer cells
Comparative diagnostic efficacy and safety of ultrasound-guided percutaneous transhepatic biopsy and endoscopic ultrasound-guided fine-needle aspiration biopsy for gallbladder tumors
Bifurcation analysis and dynamical behavior of novel optical soliton solution of chiral (2 + 1) dimensional nonlinear Schrodinger equation in telecommunication system
Voice biomarkers as prognostic indicators for Parkinson’s disease using machine learning techniques
Implantation of a vaccine platform for extended antigen release (VPEAR) induces long-term immunity against Haemonchus contortus in sheep
Examining the role of compression in influencing AI-generated image authenticity
Cancer vulnerabilities exposed by finding interactions among DNA repair factors
Green jackfruit flour ameliorates MASH and development of HCC via the AMPK and MAPK signaling pathways in experimental model systems
Presence of antibiotics in children’s urine: a silent risk beyond drinking water
Neural insights on expert surgeons’ mental workload during live robotic surgeries
Research on failure properties and analysis methods of Q690D based on energy characteristics of AE signal
Time lag effect of precipitation on groundwater level based on wavelet analysis in the People’s Victory Canal irrigation area, China
Jurassic fossil reveals the origin of parasitic thorny-headed worms
The relationship between ethical climate and organizational cynicism: mediating role of match and identification with the organization
Abstract Depending on its type, the ethical climate of an organization has either beneficial or detrimental effects in the workplace. However, there is a lack of knowledge about the role of employees’ attachment to the organization and the coherency of values between employees and organizations in the relationship between different types of ethical climates and negative attitudes toward the organization. This cross-sectional study tested the mechanisms of the direct and indirect effects of ethical climate on organizational cynicism through person–organization fit, organizational pride, and affective commitment, attempting to determine the protective function of different ethical climates on organizational cynicism, which is an undesirable workplace phenomenon. The study focused on 1071 Polish employees from different business areas with contracts of employment. The results showed that an instrumental ethical climate had positive direct and indirect effects on organizational cynicism through all three mediators, person–organization fit, organizational pride, and affective commitment. Moreover, beneficial indirect effects of caring and independent climates on organizational cynicism through person–organization fit, organizational pride, and affective commitment were also confirmed. Law and code and rules climates were positively and indirectly related to organizational cynicism via organizational pride but not organizational affective commitment. Discussed the impact of different ethical climates on organizational cynicism and the beneficial role of person-organization fit, organizational pride, and affective commitment underlying this relationship, emphasizing the preventing function of organizational attachment, organizational pride, and values consistency between employee and organization in referring cynical attitudes in the workplace.