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Early activity after strong sutures helps to tendon healing in a rat tendon rupture model
Amblyopic binocular imbalance quantified by the dichoptic contrast ordering test and dichoptic letters test
Mutational signature analysis predicts bacterial hypermutation and multidrug resistance
Influence of rock heterogeneity on the correlation between uniaxial compressive strength and Brazilian tensile strength
Non-targeted LC-MS metabolomics reveals serum metabolites for high-altitude adaptation in Tibetan donkeys
Synergistic enhancement of electrochemical alcohol oxidation by combining NiV-layered double hydroxide with an aminoxyl radical
High temperature QDs organization and re-crystallization in glass supported MgO QDs doped PMMA film
Hydrological dynamics of the Yangtze river-Dongting lake system after the construction of the three Gorges dam
Drug inhibition and substrate transport mechanisms of human VMAT2
Factors associated with vaccine default in Southern Ghana based on data from the RTSS malaria vaccine trial in Cape Coast
Integrative pan-cancer analysis and experiment validation identified GLS as a biomarker in tumor progression, prognosis, immune microenvironment, and immunotherapy
Supramolecular modification of sustainable high-molar-mass polymers for improved processing and performance
Molecular and biological characterization of infectious full-length cDNA clones of two viruses in Paris yunnanensis, including a novel potyvirus
Biological functions of extracellular vesicle double C2-like domain beta in cervical cancer
Abstract Double C-2 Like Domain Beta (DOC2B) located at 17q13.3 prevents metastasis by senescence induction and epithelial to mesenchymal transition inhibition in cervical cancer (CC). The extracellular vesicle (EV) mediated trafficking of DOC2B and its impact on tumor suppressive activity are not investigated in CC. Using a retroviral method, we first ectopically expressed DOC2B in SiHa, which do not normally express DOC2B. DOC2B-SiHa and vector-SiHa EVs were co-incubated separately with recipient cell and subjected to various cellular and biochemical experiments. For the first time, we demonstrated that DOC2B localizes to EVs, and its transfer to EV may require intracellular calcium. Co-culture of SiHa and HeLa cells with DOC2B-SiHa derived EVs induced morphological changes and suppressed their growth and migration, possibly by induction of G0/G1 to S phase arrest and anoikis. DOC2B-SiHa EVs elevated intracellular reactive oxygen species (ROS) and calcium levels and promoted lipid droplet accumulation and lipid peroxidation rate in recipient cells. DOC2B-SiHa EVs reduced active AKT1 and ERK1/2 levels and EMT marker expression and enhanced cellular senescence and cytotoxic effects of cisplatin. Re-expression of DOC2B significantly altered the global metabolite profile of EVs. Finally, we demonstrated that intracellular calcium chelation significantly reduces DOC2B localization to EVs and impacts its tumor-suppressive properties. Altogether, EV-mediated DOC2B transfer may reduce the aggressive behavior of CC cells.