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Bayesian inference by visuomotor neurons in the prefrontal cortex
Perceptual judgments of the environment emerge from the concerted activity of neural populations in decision-making areas downstream of the sensory cortex. When the sensory input is ambiguous, perceptual judgments can be biased by prior expectations shaped by environmental regularities. These effects are examples of Bayesian inference, a reasoning method in which prior knowledge is leveraged to optimize uncertain decisions. However, it is not known how decision-making circuits combine sensory signals and prior expectations to form a perceptual decision. Here, we study neural population activity in the prefrontal cortex of macaque monkeys trained to report perceptual judgments of ambiguous visual stimuli under two different stimulus distributions. We isolate the component of the neural population response that represents the formation of the perceptual decision (the decision variable, DV), and find that its dynamical evolution reflects the integration of sensory signals and prior expectations. Prior expectations impact the DV’s trajectory both before and during stimulus presentation such that DV trajectories with a smaller dynamic range result in more biased and less sensitive perceptual decisions. We show that these results resemble a specific variant of Bayesian inference known as approximate hierarchical inference. Our findings expand our understanding of the mechanisms by which prefrontal circuits can execute Bayesian inference.
Structural insights into the dual Ca2+-sensor-mediated activation of the PPEF phosphatase family
Comparative evaluation of Allplex HPV28 and Anyplex II HPV28 assays for high-risk HPV genotyping in cervical samples
Background/Objectives Human papillomavirus (HPV) genotyping is essential for cervical cancer screening and prevention. The AllplexTM HPV28 real-time PCR kit, using different chemistry and results analysis compared with its predecessor, the AnyplexTM II HPV28 kit, has recently been launched. This study aims to compare the AllplexTM HPV28 and AnyplexTM II HPV28 assays in detecting and genotyping the 13 high-risk (HR)-HPV types. Study design Between 2022 and 2023, 459 cervical samples from women undergoing cervical cancer screening were selected. These samples were analysed by liquid-based cytology and tested by both kits concurrently. Results AllplexTM HPV28 Ct values correlated well with AnyplexTM II HPV28 signal intensity scores. No significant differences between assays were observed in overall and genotype-specific HR-HPV prevalence determined in all samples and according to cytological results. In addition, no significant differences were identified between assays in the detection of single and multiple HR-HPV infections. Most of the discordant results corresponded to samples showing weak HR-HPV signals and multiple HR-HPV types. Conclusions Our results demonstrate that the AllplexTM HPV28 kit can be used for HPV genotyping, with results overall similar to those obtained with the AnyplexTM II HPV28 kit and the addition of Ct values for patient follow-up. The clinical implications of the potentially reduced sensitivity of the AllplexTM HPV28 kit in detecting HPV31 (p = 0.07) and HPV39 (p = 0.08) warrant further investigation in subsequent studies.
An adaptive search mechanism with convolutional learning networks for online social media text summarization and classification model
Cholesterol-dependent enzyme activity of human TSPO1
The amino acid sequence of the tryptophan-rich sensory proteins (TSPO) is substantially conserved throughout all kingdoms of life. Human mitochondrial TSPO1 ( Hs TSPO1) binds to porphyrins and steroids, although its interactions with these molecules remains unknown. Hs TSPO1 is associated with numerous physiological and pathological disorders, but the underlying molecular mechanisms are unknown. Here, we disclose the finding of human mitochondrial TSPO as a cholesterol-dependent protoporphyrin IX oxygenase. The results of our biochemical characterization are consistent with structural data and evolutionary analysis. The dependence of Hs TSPO1 activity on cholesterol may be the result of the coevolution of this membrane protein with the membrane system. Our study provides a molecular foundation for comprehending the various roles played by mitochondrial TSPO in normal physiological and pathological situations.
Sulfur defect engineering controls Li2S crystal orientation towards dendrite-free lithium metal batteries
Abstract Controlling nucleation and growth of Li is crucial to avoid dendrite formation for practical applications of lithium metal batteries. Li2S has been exemplified to promote Li transport, but its crystal orientation significantly influences the Li deposition behaviors. Here, we investigate the interactions between Li and various surface structures of Li2S, and reveal that the Li2S(111) plane exhibits the highest Li affinity and the lowest diffusion barrier, leading to dense Li deposition. Using sulfur defect engineering for Li2S crystal orientation control, we construct three-dimensional vertically oriented Li2S(111)@Cu nanorod arrays as a Li metal electrode substrate and identify a substrate-dependent Li nucleation process and a facet-dependent growth mode. Furthermore, we demonstrate the versatility of the Li2S(111)@Cu substrate when paired with two positive electrodes: achieving an initial discharge capacity of 138.8 mAh g–1 with 88% capacity retention after 400 cycles at 83.5 mA g–1 with LiFePO4, and an initial discharge capacity of 181 mAh g–1 with 80% capacity retention after 160 cycles at 60 mA g–1 with commercial LiNi0.8Co0.1Mn0.1O2 positive electrode (4 mAh cm–2).
Intermittent hypoxic stimulation promotes efficient expression of Hypoxia-inducible factor-1α and exerts a chondroprotective effect in an animal osteoarthritis model
Hypoxia-inducible factor-1α plays an important role in the homeostasis of articular cartilage in hypoxic environments. Therefore, modulation of hypoxia-inducible factor-1α by regulating the oxygen environment could be a useful treatment for osteoarthritis. This study aimed to assess the chondroprotective effects of intermittent hypoxia on cultured chondrocytes and an animal model of osteoarthritis. In vitro, human chondrocytes were exposed to 2 h of hypoxic stimulation three times at 1-h intervals, and protein and gene expression of hypoxia-inducible factor-1α, ACAN, and cell viability was measured over time. In vivo, 8-week-old male Wistar rats were injected with monosodium iodoacetate to induce osteoarthritis and then reared in 12% hypoxia for 24 h, followed by 24 h in steady oxygen, repeated alternately for a total of 28 days. A histological analysis was performed on days 8 and 28. In the intermittent hypoxia group, each protein expression increased with each repeated hypoxic stimulation to human chondrocytes; finally, the protein level was significantly higher with intermittent hypoxia than with continuous hypoxic stimulation, cell viability was increased, and gene expression was not significantly increased. In the osteoarthritis animal model, for 8 days, there were stronger hypoxia-inducible factor-1α staining and no significant differences in articular cartilage destruction. Furthermore, for 28 days, there was significantly less articular cartilage destruction in the rat osteoarthritis model with intermittent hypoxia than with steady oxygen rearing. Intermittent hypoxia increased cartilage metabolism by increasing hypoxia-inducible factor-1α proteins in articular chondrocytes, which may be effective in preventing articular cartilage degeneration in a rat osteoarthritis model.
Immobilization of silver nanoparticles and silver iodide within bamboo fabrics for wastewater treatment
Abstract Pathogenic microorganisms and dyes are the main sources of water pollution. These pollutants are extremely hazardous and may harm aquatic life and human health. As a result, removing these pollutants is critical in assessing contamination risks and mitigating potential health hazards. To effectively remove pathogenic microorganisms and dyes from wastewater, an efficient multi-functional material was designed based on AgI, Ag NPs, and Ag NPs@AgI immobilized on bamboo fabrics as a support substrate. The water disinfection aptitude of the modified bamboo fabrics was evaluated against different microorganisms. The results showed that the Ag NPs@AgI@bamboo showed excellent antibacterial activity against S. aureus (88%) and E. coli (90%) as well as perfect antifungal activity against C. albicans (82%). Methylene blue (MB) was used as a pollutant model to test the catalytic and photocatalytic activity of modified bamboo fabrics. The results show that Ag NPs@AgI@bamboo was highly efficient in removing the MB dye via reduction (90%) after 60 min or photodegradation (93%) after 6 h of UV light irradiation. The pseudo-first-order kinetic study shows that Ag NPs@AgI@bamboo possessed outstanding catalytic reduction and photocatalytic degradation activities toward MB.
Climate change impacts have potentially big repercussions for kids’ education
Core microbes regulate plant-soil resilience by maintaining network resilience during long-term restoration of alpine grasslands
Associations of subjective age trajectories with loneliness and stress across adulthood
Subjective age, that is the age a person feels like in relation to their chronological age, is indicative of a variety of biological, psychological, and social aging processes. Despite its importance, studies that investigate multi-variate, dynamic, longitudinal relations of subjective age with its potential determinants and potential mechanisms of these relations have so far rarely been employed. In the current study, we focus on loneliness as a potential subjective age determinant. As loneliness affects a variety of psychosocial and health outcomes across life and is stereotypically perceived as a feature of old age, we investigate whether loneliness is related with levels and changes in subjective age. We furthermore test whether this association is mediated via self-reported stress. N = 5,594 participants aged 18 – 93 years (Mage = 50.41, SD = 15.99) who participated in a longitudinal survey comprising up to three measurement occasions over a time span of 2.5 years reported their loneliness, subjective age, and stress as well as sociodemographic and health-related covariates. We employed latent growth modeling and found that, when controlling for sociodemographic and health-related covariates, higher loneliness was related to an older subjective age cross-sectionally and to a steeper increase in subjective age over time. These relations were mediated via stress; however, the relation between stress and subjective age was no longer statistically significant when including the covariates. All associations were qualified by significant interactions with chronological age, albeit in different directions. Our findings attest to associations between loneliness, stress and subjective aging experiences and highlight the need for an age-informed approach when planning further studies and interventions.
Insecticidal effects of nano-encapsulated lemongrass essential oil on the population parameters of Spodoptera frugiperda using two-sex life table
Abstract Essential oils derived from plants have demonstrated their efficacy in controlling insects; however, their poor physical characteristics restrict their widespread application. In this study, nano-encapsulation employing emulsification followed by ultrasonication technique has improved the insecticidal efficacy of lemongrass oil (LGO) against Spodoptera frugiperda. Beta-cyclodextrin (β-CD) and gum Arabic (GA) were used as coating materials to protect the vital components of LGO. Different concentrations of LGO pure and nano-capsule formulations have been used to examine the biological development of S. frugiperda 1st larval instar using two sex life table. D-Limonene, E-Citral, and Z-Citral were identified as the main compounds in LGO by GC–MS analysis. The oil concentration affected the physical properties of nano-capsules. The LGO nano-capsules had particle sizes ranging from 279.63 to 471.93 nm and an encapsulation efficiency between 51.94 and 83.59%. Following 96 h, the LGO:β-CD/GA treatment of S. frugiperda had a lower LC50 value (1.52%) than the LGO treatment (2.48%), indicating that the nano-capsules were more toxic. Nano-capsules had a significant adverse effect on the life table parameters of S. frugiperda. The results of this study indicate that encapsulating LGO with β-CD/GA inclusion enhanced the efficacy of lemongrass oil against S. frugiperda.
Allosteric mechanism in the distinctive coupling of G <sub>q</sub> and G <sub>s</sub> to the parathyroid hormone type 1 receptor
The mechanism determining the preferential stimulation of one heterotrimeric G protein signaling pathway over another by a ligand remains undetermined. By reporting the cryogenic electron microscopy (cryo-EM) structure of the parathyroid hormone (PTH) type 1 receptor (PTH1R) complexed with Gq and comparing its allosteric dynamics with that of PTH1R in complex with G s , we uncover a mechanism underlying such preferences. We show that an allosteric coupling between the ligand PTH and the C-terminal helix α5 of the Gα subunit controls the stability of the PTH1R complex with the specific G protein, G s or G q . Single-cell-level experiments further validate the G protein–selective effects of the PTH binding pose by demonstrating the differential, G protein–dependent residence times and affinity of this ligand at the PTH1R binding site. The findings deepen our understanding of the selective coupling of PTH1R to G s or G q and how it relates to the stability and kinetics of ligand binding. They explain the observed variability in the ligand-binding affinity of a GPCR when coupled to different G proteins.
Long COVID after SARS-CoV-2 during pregnancy in the United States
Does physical activity-based intervention decrease repetitive negative thinking? A systematic review
Background Repetitive negative thinking (RNT) is characterized by its persistence, difficulty in control, and the tendency to focus on negative thoughts and past events. It is recognized as a key factor in the development and maintenance of mental health issues such as depression and anxiety. A growing body of research suggests that physical activity-based interventions may effectively reduce RNT. However, the extent of this effect and the mechanisms behind it remain inconsistent across studies. Methods This systematic review synthesized evidence from 19 peer-reviewed studies retrieved from PubMed, Web of Science, and other relevant databases up to December 2024. The objective was to investigate the efficacy of physical activity-based interventions in reducing RNT, with a particular focus on the influence of intervention type, duration, frequency, and intensity. Results The review found that physical activity interventions effectively reduce RNT, particularly when combined with psychological training. Combined interventions yielded greater reductions than physical activity alone. Moderate-to-high intensity exercise (30-60 min/session, 3-5 times/week) over an extended period was most effective, likely due to physiological, psychological, and social mechanisms. Single-session interventions showed limited effects, emphasizing the need for sustained engagement. Notably, interventions were more effective in individuals with depression, anxiety, or chronic stress, whereas effects in healthy individuals were smaller and more variable, suggesting that baseline symptomatology enhances intervention benefits. Conclusions This review underscores the importance of designing intervention protocols that integrate both physical and psychological components to achieve greater reductions in RNT. The findings provide empirical support for the use of combined interventions involving physical activity and psychological training as an effective strategy for managing RNT. Additionally, future research should prioritize identifying optimal intervention characteristics (e.g., type, frequency, intensity) and addressing methodological limitations, such as the inclusion of diverse participant samples and broader language coverage, to provide more comprehensive insights into effective intervention strategies. These findings have important implications for mental health interventions and offer practical guidance for developing evidence-based approaches to reduce RNT.
Object detection model design for tiny road surface damage
Enhancing immunotherapy with tumour-responsive nanomaterials
The PVD neuron has male-specific structure and mating function in <i>Caenorhabditis elegans</i>
Neurons display unique shapes and establish intricate networks, which may differ between sexes. In complex organisms, studying sex differences in structure and function of individual neurons is difficult. The nematode Caenorhabditis elegans hermaphrodites and males present an exceptional model for studying neuronal morphogenesis in a simple, sexually dimorphic system. We focus on the polymodal sensory bilateral neuron pair PVD, which forms a complex but stereotypic dendritic tree composed of multiple subunits that resemble candelabra. PVD is well studied in hermaphrodites, but not in males. We show here that during larval development, male PVD extends a similar architecture to the hermaphrodite utilizing the sexually shared Menorin patterning mechanism. In early adulthood, however, male PVD develops a unique extension into the copulatory tail structure. Alongside established tail ray neurons RnA and RnB, we show PVD is a third, previously unrecognized, neuron within the tail rays. Unlike RnA and RnB, PVD extends anterogradely, branches and turns within the ray hypodermis, and is nonciliated. This PVD sexually dimorphic arborization is absent in mutant backgrounds which perturb the Menorin guidance complex. SAX-7/L1CAM, a hypodermal component of this complex, shows a male-specific expression pattern which precedes PVD extension, and its presence allows PVD to enter the tail rays. Further, our results reveal that genetically altered arborization or ablation of the PVD results in male mating behavioral defects, particularly as males turn around the hermaphrodite. These results uncover an adult-stage sexual dimorphism of dendritic branching and a function for PVD in male sexual behavior.