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Explicit solitary wave structure for the stochastic resonance nonlinear Schrödinger equation under Brownian motion with dynamical analysis
Inequalities and trends in access to health insurance and essential public health services among internal migrants in china: 2013 to 2018
GC-MS and HPLC chemical profile, antioxidant, anti-acetylcholinesterase, and anti-diabetic activities of Libyan Salvia lanigera herb extract and essential oil
Abstract The current study explored the chemical composition and biological activities of the essential oil and ethanol extract of S alvia lanigera collected from Libya. The ethanol extract obtained from the wild growing S . lanigera was evaluated for the chemical composition via high-performance liquid chromatography with diode-array detection (HPLC–DAD), which demonstrated phenolic and flavonoid contents. A total of 17 compounds, representing phenolic and flavonoid derivatives, have been identified. Besides, gas chromatography–mass spectrometry (GC–MS) analysis of the essential oil obtained from S. lanigera aerial parts identified 24 compounds, accounting for 99.33% of the oil, and the major components were characterized to be 1,8-cineole (27.28%), camphor (25.82%), α-pinene (7.71%), and α-terpineol (7.67%). The results indicated that the oil and ethanol extract exhibited marked radical scavenging capacity toward DPPH (2,2-diphenyl-1-picrylhydrazyl), with IC 50 values of 0.1337 and 0.6331 µg/mL, respectively. Further, the oil and ethanol extract demonstrated potent activity against ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), with IC 50 values of 0.17 and 0.0501 µg/mL, respectively. Moreover, S. lanigera oil inhibited more than 76% of the acetylcholinesterase enzyme activity, with an IC 50 value of 144 ± 1.04 µg/mL. The in vitro data showed that the ethanol extract has potential antidiabetic properties toward the α -glucosidase enzyme with an IC 50 value of 124.6 ± 1.07 µg/mL. Regarding the nature of measurement, assay conditions, and ligand-specific factors such as solubility and stability, we demonstrated a correlation between the IC 50 values and in-silico results for antidiabetic assays, where chlorogenic and rosmarinic acids recorded the highest docking scores of -9.4 and − 8.9 kcal/mol, respectively. However, this correlation was not observed in the anti-cholinesterase assay. Further in vivo investigations are essential to confirm the in vitro results of S. lanigera oil and ethanolic extract. This study helps elucidate the potential therapeutic applications of these compounds and their effectiveness in managing diabetes and neurodegenerative disorders.
Car4-positive adipocyte progenitor cells adapt to the aging environment and work as protection against ROS via glutathione metabolism
Abstract The induction of beige adipocytes is significantly reduced in aged mice due to the senescence of adipocyte progenitor cells (APCs). Recent studies have revealed the existence of beige adipocyte subtypes, suggesting that APCs comprise a heterogeneous population. Therefore, in this study, we aimed to elucidate the mechanism through which long-term cold exposure induces the production of beige adipocytes even in aged mice. Single-cell RNA sequencing identified carbonic anhydrase 4 (Car4)-positive APCs. The number of Car4-positive APCs increased with age and cold exposure. Car4 knockdown (KD) mitigated intracellular pH reduction and significantly suppressed beige adipocyte differentiation. Furthermore, Car4 KD cells demonstrated reduced expression of genes in the glutathione pathway and increased susceptibility to reactive oxygen species (ROS), which was alleviated by glutathione supplementation. Our findings suggest that ROS resistance is an adaptation to the cellular aging environment. Our study provides insights into the age-related decline in beige adipocyte induction and identifies Car4 as a potential therapeutic target for enhancing energy expenditure in elderly individuals. This may pave the way for the development of new strategies to combat age-related metabolic diseases and offer hope for improved health and longevity in an aging population.
GC-MS profiling, in vitro and in silico antibacterial and antioxidant potential of crude secondary metabolites of an endophytic bacterium isolated from Enhydra fluctuans lour
Pilot study of dynamic lighting and sleep consolidation among older adults in a Jordanian senior care facility
Evaluating the effectiveness of Transcendental Meditation on mental health and resilience of paramedicine students – a randomised controlled pilot study
Adipose tissue gene expression and longitudinal clinical phenotypes are early biomarkers of lipid-regulating drug usage
Abstract Cardiovascular disease progression is characterised by the dysregulation of lipid metabolism and pro-atherogenic effects of adipose tissue signalling. Recent findings from the analysis of transcriptomic data in bulk tissue has enabled these insights and revealed important changes in gene expression. However, few studies have explored these molecular mechanisms before the onset of cardiovascular disease. We explore associations between future lipid-regulating drug use and cardiometabolic traits (n = 103), including DXA scans of body composition at baseline and follow-up 5–10 years later, in a cohort of British twins (n up to 6963). Utilising transcriptomic profiles from a subset of twins (n = 766), we explore the associations between baseline adipose tissue gene expression, clinical traits, and future lipid-regulating drug usage. We then test the joint predictive capacity of clinical traits plus gene expression compared to traditional risk scores using an automated machine learning approach. We find 44 traits are associated with lipid-regulating drug usage including measurements of abdominal fat tissue, cardiovascular health, and lipid metabolism (FDR 5%). Then, we present that adipose tissue gene expression levels at baseline are associated cross-sectionally with 19 of these 44 traits (FDR 5%). By comparing adipose gene expression levels between individuals prescribed lipid-regulating drugs in the future and controls, we discover that genes associated with 16 of these 19 traits produced greater log2-fold changes, suggesting shared mechanisms. We reveal 15 differentially expressed genes comparing future lipid-regulating drug users and controls at baseline (FDR 10%), including some implicated in angiogenesis: ESM1, RCAN2, and SOCS3. Functional enrichment with 1212 significantly differentially expressed genes (p < 0.05) included molecular mechanisms related to abnormal cardiovascular system electrophysiology (p = 1.89 × 10−3), arrhythmia (p = 4.02 × 10−3), and mitochondrial pathways (p = 1.12 × 10−3). Finally, we confirm inclusion of gene expression levels as features in machine learning models achieves a better AUC (0.919) compared to traditional risk predictors. These findings highlight the potential of bulk transcriptomic data to improve risk stratification for lipid-regulating drug use, offering new insights into the RNA biology of adipose tissue and advancing approaches for cardiovascular disease prevention.
Solar peculiar motion inferred from dipole anisotropy in redshift distribution of quasars appears to lie along the Galactic Centre direction
Technical research on surrounding rock control of roadways crossing collapse columns in strong mine pressure working faces
A novel chromen-based small molecule induces apoptosis and modulates cellular response to triple-negative breast cancer
Data-driven analysis of the relationship between the HbA1c/HDL-C ratio and coronary artery calcification: a cross-sectional study
AntimiR uptake by human proximal tubule epithelial cells is predominantly by macropinocytosis
Abstract The ease with which microRNA inhibitors (antimiRs) can be delivered varies with the intended target cell type and tissue. AntimiRs are of interest as potential therapeutics for kidney conditions, including ischaemia–reperfusion injury in transplantation. During ex-situ human kidney perfusion, antimiRs are delivered to the proximal tubule epithelium without the use of transfection reagents by an endocytic process. Here we investigate whether antimiR uptake by proximal tubule epithelial cells (PTEC) occurs in vitro, without transfection reagents, and which endocytic entry mechanism is responsible. PTEC were isolated from human kidneys declined for transplantation and maintained in culture. PTEC were shown to take up antimiR through detection of increased fluorescent signal associated with the antimiR label. A vesicular pattern of uptake was demonstrated on fluorescence microscopy, in keeping with endocytic uptake. Endocytosis was confirmed by co-occurrence of the antimiR with endocytic markers and temperature dependence of uptake. Megalin inhibition with receptor associated protein to target receptor-mediated endocytosis had no effect on antimiR uptake, whereas the macropinocytosis inhibitor 5-(N-ethyl-N-isopropyl)-amiloride reduced antimiR uptake. Our results demonstrate antimiR can be delivered to primary human PTEC in vitro without the use of transfection reagents and support macropinocytosis as the dominant pathway of antimiR entry.
Self-relevant facial threat attracts peripheral attention
Choline transporter-like protein 1 in tongue squamous cell carcinoma: implications for cell proliferation and differentiation
Bauhinia monandra derived mesoporous activated carbon for the efficient adsorptive removal of phenol from wastewater
Abstract The quality evaluation of the activated carbon made from agricultural waste is investigated in the adsorption of phenol. Adsorbent was prepared through the activation of orthophosphoric acid, producing a structure that is porous with a surface area of 1090 m2/g. Elemental composition analysis confirmed that the carbon composition is 78.23%. X-ray photoelectron spectroscopy (XPS) and other characterizations were conducted to study the structural, chemical and thermal performance metrics. The results from the Boehm titration were aligned with the point of zero charge. Response Surface Methodology was used to optimize adsorption parameters. The adsorption outcomes demonstrated the effectiveness of phenolic removal, with the Langmuir isotherm under optimal conditions exhibiting a maximum adsorption capacity of 185.93 mg/g. The study on the kinetics of adsorption showed that they closely matched the second-order pseudo model (R2 > 0.98). Thermodynamic studies indicate that adsorption occurs spontaneously, as evidenced by negative ΔG° values. This further validates its exothermic nature and decreases randomness at the solid–liquid interface, as indicated by negative ΔH° and ΔS° values. The adsorbent regeneration and spiking studies were methodically examined. Activation energy and isosteric heat of adsorption were assessed. The adsorption mechanism is discussed thoroughly. These results highlight the effectiveness of activated carbon as a practical adsorbent for treating wastewater.