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Exploring the anti-obesity potential of Ailanthus excelsa Roxb in vitro enzymatic inhibition and computational pharmacology insights
Cyclic Hydroxylamines for Native Residue-Forming Peptide Ligations: Synthesis of Ubiquitin and Tirzepatide
Digestion in the arachnid Mischonyx squalidus as a probable source of lipids to synthesize opiliones defense and communication molecules
Long-Lived Hole Accumulation in Al:SrTiO<sub>3</sub>/Rh–Cr Photocatalyst Systems under Continuous Irradiation and Its Correlation with Overall Water Splitting Efficiency
In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy
Abstract Single molecule localization microscopy (SMLM) allows deciphering the nanoscale organization and dynamics of biomolecules in their native environment with unprecedented resolution. While SMLM was quickly adopted by the scientific community for its performance and simple instrumentation, it still remains limited in its in-depth capability, precluding many biological processes to be investigated. We here present a solution to perform in-depth volumetric SMLM, called soSMARt. It relies on innovative microfabricated devices allowing both single-objective light-sheet microscopy, aberrations correction via adaptive optics, and real-time feedback-loop registration with nanometric precision. We illustrated the performances of soSMARt to assess the 3D nanoscale organization of several protein of interest in isolated cells, and explore optimizations and proof-of-concepts for the investigation of more complex tissues such as 3D cell cultures. We believe our method addresses key limitations of single molecule microscopy, paving the way for novel biological applications.
A repeated awakening study exploring the capacity of complexity measures to capture dreaming during propofol sedation
Abstract Patients undergoing general anesthesia are often assumed to be unconscious. However, it is known that conscious experiences in the form of dreams occur even in unresponsive states induced by anesthetics. Here, we recorded resting state electroencephalography (EEG) as well as EEG combined with TMS perturbations in 20 healthy participants during propofol sedation. Participants were repeatedly awoken from deep sedation and asked immediately whether they had experiences just before waking up and what they experienced. Out of the 52 attempted awakenings in this study, 24 produced reports of having had an experience, while there were 5 reports of no experience. In the remaining 23 attempts, the subject was either unarousable or the report too incoherent to provide information about their experience prior to awakening. We then tested whether two different consciousness measures based on EEG complexity—the state transitions perturbational complexity index (PCIst) and single-channel Lempel-Ziv complexity (LZc)—differed between awakenings with and without experience. While our study confirms earlier findings that the EEG complexity measures significantly decrease from the awake state to the sedated state, we find no evidence that these measures differ between periods associated with reports of dreaming and non-dreaming, within the sedated state. A few interpretations and limitations are discussed.
Tailoring Near Fermi-Level Topological Flatbands in Clar’s Goblet Graphene Nanoribbons through Regioselective Cyclization of Five-Membered Rings
Development of deep learning-based narrow-band imaging endocytoscopic classification for predicting colorectal lesions from a retrospective study
Examining regional disparities in maternal and child health in Bangladesh using cluster analysis of MICS 2019 data
Type-I Supramolecular Photosensitizer Enables GSH Depletion by Hydrogen Atom Transfer
Author Correction: Guanidine aptamers are present in vertebrate RNAs associated with calcium signaling and neuromuscular function
Unprecedented expansion of graphite with low power laser for high-quality liquid phase exfoliated graphene
Abstract As graphene and related materials increasingly integrate into various industries, producing high-quality graphene sheets on a large scale becomes crucial. Here, we present a unique approach to the large-scale production of graphene through laser-assisted graphite expansion followed by ultrasonic exfoliation. The present method utilizes laser technology to significantly expand graphite, achieving an expansion rate of 800 mL g−1 with a deficient energy consumption of just two watts laser. The graphene produced via ultrasonication of expanded graphite samples exhibited high quality (ID/IG) ~ 0.13 and a few layers with a (I2D/IG) ~ 0.52. Free-standing films with different thicknesses (11–69 µm) were successfully prepared through filtration, reaching significant electrical conductivity up to (~ 1707 S cm−1). The as-prepared graphene films are further explored for electromagnetic interference (EMI) shielding. The highest shielding effectiveness (SE) was recorded for the graphene film with a thickness of 69 µm, reaching a value of ~ 72 dB. Meanwhile, the graphene film with an 11 µm thickness achieves the highest absolute effectiveness (SSE/t) of ~ 58,666 dB cm2 g−1 , surpassing most current graphene and MXene films, which typically present values in the range of 10,000 to 40,000 dB cm2 g−1. This present laser-assisted expansion incorporating a sonication exfoliation strategy paves a new way to produce graphene on a large scale.