Browse Articles

Diagnostic assistance method for RR-TB/MDR-TB patients under treatment based on CNN-LSTM

Evaluation of movable tight oil in different reservoir types in the Central Sichuan region

Impact of rotating fan and ultrasonic humidifier on the thermal performance of pyramidal solar still

Abstract This study investigates the performance enhancement of a pyramidal solar still by integrating two active techniques: a low-power fan and an ultrasonic humidifier. Baseline experiments were first conducted using a static pyramidal solar still and compared with results from a fan-assisted configuration and a unit equipped with an ultrasonic humidifier. The fan promoted faster vapor circulation, while the humidifier produced fine mist particles that accelerated evaporation. Experiments carried out under Dubai’s climatic conditions showed that the system achieved a maximum efficiency of 57%, with corresponding values of heat transfer rate, evaporative heat transfer coefficient, and condensation heat transfer coefficient of 148.43 W, 20.38 W/m²°C, and 1.64 W/m²°C, respectively. The maximum partial vapor pressures at the basin and glass surfaces were 18,626.71 Pa and 15,276.53 Pa. At peak heating around 3:00 PM, the ultrasonic humidifier-based system recorded significantly higher partial vapor pressures—38.04% at the basin surface and 23.87% at the glass surface—compared to the static still, highlighting the effectiveness of active enhancements in accelerating vapor generation. Overall, the ultrasonic-based system demonstrated the highest performance, while the static unit produced the lowest output. The distillate yields were 90 ml, 180 ml, and 330 ml for the static, dynamic, and ultrasonic systems, respectively. Also, it is found that, the system yielded energy and exergy efficiencies equal to 25.11% and 1.75%.

Solvent controlled synthesis of spiro and nonspiro indolinones with aminonaphthoquinone and pyrazolone moieties

Real-world survival and prognostic factors in WHO 2021 classified gliomas treated with chemo-radiotherapy

A mining-area surface-subsidence prediction method based on SBAS-InSAR and STL-XGBoost

Analgesic and anti-inflammatory potential of Ficus Palmata Forssk.: an In-vitro and In-vivo approach

Improving stage-discharge relationship modeling accuracy using a hybrid ViT-CNN framework

Biomechanical evaluation of nursing cart handles using electromyography

Resolution sequence stratigraphic division of the Da2 member in the Yanji Basin based on INPEFA and Milankovitch cycles

Complete mitochondrial genomes of Axis axis and Rusa unicolor from Tamil Nadu, India and comprehensive analysis with other Cervidae

Cost-effective method for semi-quantitative analysis of soluble endoglin in biological samples after anti-endoglin monoclonal antibody treatment

Abstract Soluble endoglin (sENG) is an important biomarker of several cardiometabolic and vascular disorders. The accurate measurement of biomarkers that are simultaneously targeted by therapeutic monoclonal antibodies (mAbs) in preclinical models is a significant challenge. Traditional enzyme-linked immunosorbent assays (ELISAs) often fail due to epitope blocking, while advanced techniques like mass spectrometry are more expensive and require specialized expertise. To address these limitations, we developed a cost-effective, specific Western blot-based method for evaluating sENG in mouse plasma in a metabolic dysfunction-associated steatohepatitis (MASH) rescue model. Thus, from a methodological perspective, we significantly modified and extended the sENG detection method introduced in our previous work, focusing on developing a cost-effective approach for semi-quantitative analysis. For these purposes, we used a mouse MASH model treated with the anti-endoglin (ENG) mAb M1043. Plasma samples were concentrated, and proteins were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and probed with an anti-ENG antibody. We verified the method using human hepatic sinusoidal endothelial cells (HHSECs) culture media treated with the therapeutic human anti-ENG monoclonal antibody, TRC105. This Western blot-based approach avoids interference from therapeutic mAbs, delivering reliable and reproducible results. This method overcomes the limitations of ELISAs and mass spectrometer, providing a practical solution for semi-quantitative analysis of biomarkers in both preclinical and clinical research. Its versatility makes it applicable to various soluble protein biomarkers across diseases. As therapeutic mAbs become more widely used, this simple, cost-effective method facilitates mechanistic insights and accelerates targeted therapy development in research.

A framework for AI ethics literacy: development, validation, and its role in fostering students’ self-rated learning competence

The effect of molecular weight of hyaluronic acid on corneal cell viability

Enhancing lymphoma cancer detection using deep transfer learning on histopathological images

PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia

The early therapeutic efficacy of robot-assisted laparoscopic radical prostatectomy with combined anterior and posterior approach: a cohort study

Multi-objective optimization of speed frame parameters for polyester spun yarn using artificial intelligence and grey relational analysis

The world’s first plastics treaty is in crisis: can it be salvaged?

Young KRAB-zinc finger gene clusters are highly dynamic incubators of ERV-driven genetic heterogeneity in mice

Abstract KRAB-zinc finger proteins (KZFPs) comprise the largest family of mammalian transcription factors, rapidly evolving within and between species. Most KZFPs in human and mice have been found to repress endogenous retroviruses (ERVs) and other retrotransposons, with KZFP gene numbers correlating with the ERV load across species, suggesting coevolution. Whether new KZFPs emerge in response to ERV invasions is currently unknown. Using a combination of long-read sequencing technologies and genome assembly, we present a detailed comparative analysis of young KZFP gene clusters in the mouse lineage, which has undergone recent KZFP gene expansion and ERV infiltration. Detailed annotation of KZFP genes in a cluster on Mus musculus Chromosome 4 reveals parallel expansion and diversification of this locus in different mouse strains (C57BL/6 J, 129S1/SvImJ and CAST/EiJ) and species ( Mus spretus and Mus pahari ). Our data supports a model by which new ERV integrations within young KZFP gene clusters likely promoted recombination events leading to the emergence of new KZFPs that repress them. At the same time, ERVs also increased their numbers by duplication instead of retrotransposition alone, unraveling a new mechanism for ERV enrichment at these loci.