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From Narrative to Navigation—A Translational Roadmap for AI-enabled Early Sepsis Prediction: Comment on Shanmugam et al.
Evolution mechanism of deformation and failure energy in composite rock mass with structural planes of different inclinations
Impact of Personalized Parenteral Nutrition on Inflammatory Markers and Clinical Outcomes in Critically Ill Patients: A Systematic Review and Meta-analysis
A deep learning-based method combines manual and non-manual features for sign language recognition
The Sci-Hub Ban in India: When Copyright Collides with Clinical Reality
Secure federated transfer learning with enhanced secure multiparty computation for privacy preserving smart EHR systems
Comparison of Sodium, Potassium, Hemoglobin, and Glucose Levels by Blood Gas Analyzer and Hospital Laboratory Autoanalyzer in Emergency Department Settings: A Cross-sectional Study
Identification of genetic variants associated with ear length in drop-eared dogs
Diagnose Pediatric Delirium Preferentially by Psychiatric Examination, as CAPD Has Low Specificity
Deciphering the molecular basis of skin color variation through transcriptomics and machine learning
C-reactive Protein/Albumin Ratio as a Predictive Inflammatory Marker for Postoperative Systemic Inflammatory Response Syndrome and/or Sepsis in Polytraumatized Patients in ICU
Redox properties of quercetin iron II complex with enhanced antioxidant and antiviral activities
Abstract Quercetin is a naturally occurring flavonoid widely recognized for its potent antioxidant, anti-inflammatory, and anticancer effects. However, its clinical application is limited by low water solubility and poor bioavailability. In this study, the complexation of quercetin with iron (II) ions was systematically investigated through electrochemical, spectroscopic, biological, and computational techniques to improve its physicochemical and pharmacological performance. Cyclic voltammetry revealed that quercetin undergoes irreversible oxidation due to semiquinone and para-quinonemethide formation, while Fe(II) exhibited quasi-reversible redox behavior, which became less reversible upon complexation, suggesting altered electron transfer kinetics. The stability constant (log βMX = 3.29) and the negative Gibbs free energy (ΔG = − 19.07 kJ/mol) confirmed spontaneous and thermodynamically favorable complex formation. UV–Vis spectroscopy showed significant bathochromic shifts (Δλ = 66 nm), and the continuous variation method established a 1:1 metal-to-ligand stoichiometry. ¹H NMR spectroscopy and X-ray diffraction patterns further confirmed the coordination sites and structural modifications upon metal binding. The Fe(II)-quercetin complex demonstrated superior antioxidant capacity in DPPH radical scavenging assays, with an IC₅₀ of 21.86 µg/mL compared to 23.47 µg/mL for free quercetin, confirming enhanced radical scavenging activity upon complexation. Cytotoxicity assays on HepG2 and MCF-7 cancer cell lines revealed enhanced bioactivity of the complex, with IC₅₀ values of 26.80 and 18.60 µM, respectively. Antifungal testing showed moderate inhibition against Candida albicans. Molecular docking simulations using the SARS-CoV-2 spike protein (PDB: 7JWY) indicated stronger binding affinity for the complex (− 5.42 kcal/mol) than quercetin alone, suggesting potential antiviral efficacy. Additionally, DFT calculations revealed increased softness and electrophilicity of the complex, supporting its enhanced reactivity. Collectively, these findings position the Fe(II)-quercetin complex as a promising multifunctional agent with potential applications in antioxidant therapy, cancer treatment, and antiviral drug development.
Head impact biomechanics across men’s and women’s contact sports: a comparative and clustering analysis
Potential categories and influences on college students’ exercise motivation: a latent profile-based analysis
Phage resistance of Bordetella avium due to an altered cell wall results in increased susceptibility to the polypeptide antibiotics colistin and polymyxin B
Abstract Infections caused by multidrug-resistant bacterial pathogens are becoming increasingly frequent, so alternative therapeutic strategies such as phage therapy are gaining in importance. However, one of the main challenges in phage therapy is the rapid emergence of phage-resistant bacteria. Hence, this study investigated the occurrence of phage resistance in Bordetella avium , the causative agent of bordetellosis in poultry of all ages, with a special focus on the association between bacterial phage resistance and antibiotic resistance. Here, we describe the isolation and analysis of four B. avium mutants that showed resistance to B. avium phages, with decreased adsorption leading to phage resistance of the mutants. SDS-PAGE analysis revealed a lipopolysaccharide (LPS) structure with altered nuclear patterns and a missing O-antigen moiety in the phage-resistant B. avium mutants. Whole-genome sequence analysis revealed mutations in the genetic loci BAV2233 and BAV2235, encoding lipopolysaccharide glucosyltransferase family 4 proteins, and BAV0511, encoding Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB protein, which are involved in LPS biosynthesis. Additionally, antimicrobial susceptibility testing against nine antibiotics using the agar dilution method showed that LPS changes in phage-resistant mutants affected the susceptibility of B. avium to two of the antibiotics examined. Specifically, the MIC values of the polymyxin antibiotics colistin and polymyxin B decreased by up to five two-fold dilutions, depending on the isolate considered. An association was observed between the length of the polysaccharide chain in LPS and the susceptibility of B. avium to colistin and polymyxin B, with shorter chains indicating higher susceptibility. Collective findings illuminate a beneficial aspect that may support the use of B. avium phages. Based on these results, genetically modified phages based on these B. avium phages would be a promising direction for future phage applications as an alternative to the use of antimicrobial agents.
Explainable AI based hybrid DRM-Net transfer learning model for breast cancer detection and classification using ultrasound images
Sex specific associations of urinary metals with TyG index in the Chinese elderly
Unravelling Egyptian blue Lily (Nymphaea nouchali) organs’ metabolome via UHPLC/PDA/ESI-QTOF-MS and in relation to their antioxidant and anti-cholinesterase effects
Abstract Nymphaea nouchali is a prevalent water lily well recognized for its dietary and traditional medicinal purposes, attributed to its diverse phytochemical composition. This study is the first to provide a comprehensive metabolite profiling of Egyptian Nymphaea organs viz., flower, leaf, and stem using UHPLC/PDA/ESI-QTOF-MS high-resolution ultra-performance liquid chromatography Electrospray ionization/ photo diode array detector/ Quadrupole time-of-flight mass spectrometry. Alongside the in-vitro antioxidant activities employing different methods viz., DPPH, ATBS, NO scavenging, hydrogen peroxide scavenging, and cholinesterase inhibitory (AChE) activity were investigated. A total of 185 secondary metabolites were annotated belonging to 10 chemical classes viz., phenolic acids, ellagitannins, flavonoids, anthocyanins, alkaloids, amino acids and vitamins, organic acids, fatty acids & amides, lipids and sugars/sugar derivatives. Among them, 72 are newly reported viz., ellagic acid, corilagin, patuletin glycosides, naringenin, eriodictyol glycosides, spermidine alkaloids, and 33 lipids in Egyptian Nymphaea. Flowers recorded the highest antioxidant activities with IC 50 values (45.15 µg/ml) for DPPH, (5.02 µg/ml) for ABTS + , (54.07 µg/ml) for NO, (55.04 µg/ml) for H 2 O 2 assays and (1.85 µg/ml) for AChE inhibition. Flowers demonstrated potential antioxidant activities in correlation to their flavonoids, anthocyanins and alkaloids. Multivariate analysis (MVA), including unsupervised tools like principal component analysis (PCA) & hierarchical cluster analysis (HCA), and supervised orthogonal projections to latent structures discriminant analysis (OPLS-DA) are likewise employed. This research is designed to deal in depth with the chemical profile of the Egyptian water lily and tried to correlate such profile with their corresponding neuroprotective effect. Furthermore, in-vivo biological studies are planned to confirm the active agent post isolation from the active organs following results based on chemometric analysis. As future work, it is highlighted to target the isolation of novel entities.