Browse Articles

Aptamer-linked immobilized sorbent assay for detection of VP1 of foot and mouth disease virus serotype O

Abstract Foot and Mouth Disease (FMD) is one of the most devastating animal diseases. Infected animals, such as swine, develop vesicles, often leading to morbidity or depopulation of exposed herds. Current FMD diagnosis relies on serologic and immuno-based methods, which commonly encounter cross-reactivity and cost issues. Therefore, a simple and portable diagnostic tool would greatly aid its detection efforts. This study generated a novel aptamer sequence targeting viral protein 1 (VP1) of serotype O FMD virus (FMDV) by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). The generated FMDV VP1-specific aptamer sequence was characterized and incorporated into an Aptamer-linked Immobilized Sorbent Assay (ALISA)-based microplate platform to detect FMDV VP1. Results showed that the aptamer, FMDV Apt, had an estimated dissociation constant ( K d ) of 14 ± 8.6 nM, showing strong affinity for VP1 of FMDV. A concentration-dependent linear relationship (R 2  = 0.9354) within a concentration range of 0.50 ng/mL – 5.0 ng/mL of FMDV VP1 protein was observed. The limit of detection (LOD) was determined to be 1.3 ng/mL, and the limit of quantification (LOQ) was approximately 4 ng/mL. Moreover, the FMD ALISA did not exhibit cross-reactivity when tested with high concentrations (500 ng/mL – 2,000 ng/mL) of non-targets, indicating its robustness and potential application in other detection platforms. The newly developed screening tool would facilitate efficient and inexpensive monitoring of potential biohazard risks posed by FMDV.

A multi-layered cryptographic trust reinforcement model against AI-driven threat propagation and zero-day cloud vulnerabilities in healthcare data ecosystems

Polygenic risk score from steroid-sensitive nephrotic syndrome GWAS indicates overlapping genetic basis with steroid-resistant cases

Uncovering the hierarchical determinants of continuous usage in online health communities: integrating meta-analysis with FUZZY-DEMATEL-AISM

A dual-stream deep learning framework for continuous sign language recognition to enhance communication accessibility in the Ha’il region

Anatomic safeguards and the low risk of vascular injury during percutaneous tricortical S1 pedicle screw fixation – a cadaver study

Abstract Percutaneous tricortical S1 pedicle screw fixation (PTSPSF) offers superior fixation compared to bicortical or monocortical methods but poses potential risks to major intrapelvic vessels. Strangely, clinical complications are rare. This study aims to elucidate the reasons for this discrepancy. In 17 fresh human cadavers, 34 pedicle screws were placed under fluoroscopic guidance, ensuring the distal two threads to exceed the anterior cortical promontory wall. 12 specimens were turned supine and anatomically dissected. The relation between the sacral promontory tip, screw tips and intrapelvic vessels was carefully examined. In 5 specimen 3D fluoroscopy was conducted to measure the distance between the screw tips and the surrounding blood vessels. No intraluminal screw placements were observed. In 2 cases (8.3%), screw tips penetrated the anterior longitudinal ligament and abutted the right common iliac vein (CIV), but without causing impression or injury of the vessel wall. In 2 other cases the ligament remained intact and the screw trajectory pointed dorsally to the CIV. Again the wall of this vessels remained intact. In 84%, the screws were surrounded by osteophytes and did not touch or point to major vessels. Osteophytes at the sacral promontory are typically not visible during lateral fluoroscopy-guided surgery, yet they are commonly present in older individuals. As demonstrated in our study, screw trajectory plays a critical role in avoiding vascular injury, with osteophytes potentially offering an added layer of protection to the right common iliac vein (CIV) and other major vessels. In a post-mortem setting, our findings suggest that even when screw tips penetrate the anterior longitudinal ligament and extend two threads beyond the bone, there is no direct injury to the walls of major blood vessels.

Climate change impacts on agricultural production in Ethiopia using panel data

Transient modeling and performance evaluation of a solar-driven HDH desalination system with phase change material storage

The dual G9a inhibitor and histamine H3 receptor antagonist A-366 improves repetitive and social behaviors and attenuates neuroinflammation in BTBR T + tf/J mice

Research on the hazard characteristics of thermal runaway fire in electric vehicle power battery pack

Correlation analysis of chest HRCT quantitative parameters, exhaled nitric oxide, and pulmonary function in patients with chronic obstructive pulmonary disease

Cross-lingual SMS spam detection using GAN-based augmentation for imbalanced datasets

Predicting non-emergency healthcare use in Australia using machine learning on longitudinal household data

Insights into the structural differences between homo- and heterodimers enriched from a cocktail of monoclonal antibodies against SARS-CoV-2

Transperineal drainage of male rectovesical pouch abscess under ultrasound guidance using a biplane endocavitary probe

Experimental study on the structural failure characteristics and load-bearing mechanism of anchored fractured rock mass

Integrating body composition analysis and machine learning for non-invasive identification of metabolic dysfunction-associated fatty liver disease: a large-scale health examination-based study

Infrared and visible image fusion algorithm based on NSCT and improved FT saliency detection

Single step nanosecond laser structuring for cost effective functional titanium surfaces with topography driven preosteoblast adhesion

Experiences in building social connectedness and sense of belonging: Voices from African migrants in Australia