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Assessment of compressive strength of eco-concrete reinforced using machine learning tools

Association between glycosylated hemoglobin and blood lead: A cross-sectional study

Background Diabetes is the most common chronic metabolic disease, affecting many people's health. Previous studies have shown a close relationship between trace elements and metabolic diseases. This study investigated the interrelationship between glycosylated hemoglobin (HbA1c) and blood lead (BPb) in adults. Method This research was carried out involving 12,049 eligible individuals aged 20 years or above from the National Health and Nutrition Examination Survey (NHANES) spanning from 2011 to 2020. Weighted linear regression models and smoothed curve fitting were employed to investigate the association between HbA1c and blood lead. Analyses were stratified based on age, sex, race, and body mass index, and threshold effects were explored using two-stage segmented linear regression models. Result Among all 12049 participants, through comprehensive adjustment of the model, this study discovered a negative association between HbA1c and blood lead. In addition, when stratified by sex, age, race, and BMI status in subgroup analysis in this study, this correlation still had specific statistical significance. In performing subgroup analyses, we found that the relationship between HbA1c and blood lead may yield distinct outcomes arise from gender disparities. In women, a significant U-shaped association exists between HbA1c and BPb. At approximately 6.6% of HbA1c value, the relationship between the two shifts from negative to positive. Conclusion This investigation proposes a “U” form association between HbA1c and BPb in American adults.

Oxygen and hydrobiological profiles of homemade manure-based tea in North Africa

Abstract Homemade manure tea (HMT) is commonly used in North Africa to enhance crop yields. Yet their physicochemical and biological characteristics remain poorly understood. This study evaluated oxygen and hydrobiological profiles of three types of HMT (bovine, ovine and poultry based, respectively noted HMTb, HMTo, HMTp) and compared them to control solutions of water and water supplemented with soluble NPK fertilizer. For these three types of HMT, oxygen and hydrobiological profiles were measured daily over a 7-day incubation period in three repeated, identical experiments, each comprising randomized treatments and five repetitions per treatment. Our results show that all HMT types rapidly transitioned to hypoxic conditions in the first 24h, shifting to anoxia between day 2 and day 7 depending on HMT type. This anoxic environment promoted denitrification and led to elevated NH4 + concentrations, suggesting the presence of anammox and microaerobic processes. Particulate organic matter contents and bacterial densities were highest in HMTp, while ciliate densities were highest in HMTb. These findings underscore the bioactive potential of HMT as fertilizers, with HMTp showing a favorable nitrogen profile beneficial for agricultural applications. To maintain aerobic conditions longer and reduce nitrogen losses and greenhouse gas emissions, we recommend passive or mechanical aeration, applying HMT during cooler hours, and stabilizing the pH of HMT. This study offers valuable insights to refine HMT preparation protocols, enhancing their use as bioactive fertilizers.

Network study of miRNA regulating traumatic heterotopic ossification

Objective Objective: To identify and analyze the microRNAs that are expressed differently (DE-miRNAs) and forecast their potential roles in the pathophysiological process of traumatic heterotopic ossification (THO). Methods We conducted RNA sequencing on six samples of normal bone and THO tissues from the patients and conducted differential expression analysis of miRNA. The biological activities of the target genes of the differentially expressed microRNAs (DE-miRNAs) were investigated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The miRNA-mRNA network was constructed using Cytoscape software, incorporating miRNAs with varying expression levels and their corresponding target genes. Results In comparison to the normal control group, a total of 84 differentially expressed microRNAs (p<0.05, |log2FC|>1) were identified, with 27 microRNAs showing up-regulation and 57 microRNAs showing down-regulation. The functional enrichment analysis revealed that the target genes of the de-mirna were primarily enriched in biological processes such as the regulation of protein stability and the management of neuromuscular process balance. Additionally, a miRNA-mRNA expression regulatory network was established. The RT-qPCR analysis revealed that miR-142-3p, miR-150-5p, miR-421, miR-625-5p, miR-675-5p, and miR-940 exhibited a decrease in expression levels in THO tissues. Nevertheless, the expression levels of miR-181c-3p, miR-320c, miR-497-5p, and miR-99a-5p were increased in THO tissues. Conclusions Our investigation has uncovered the expression patterns and projected the potential activities of differentially expressed microRNAs (DE-miRNAs) in human THO. This research may contribute to a better understanding of the underlying mechanisms and offer new possibilities for therapeutic targets in THO.

Agromorphological and biochemical characterization of mutagens induced M3 mutant lines of grasspea

WDR75: An essential protein for ribosome assembly undergoing purifying selection

Ribosomes, vital for life, consist of a large subunit and a small subunit (SSU), the latter is crucial for translation initiation and mRNA binding. The SSU processome, a 71-protein multimer in humans, is an intermediate in ribosome formation. One of its constituents, WDR75 plays a pivotal role by binding to an evolutionary conserved motif in the external transcribed spacer region of the rRNA to help form the SSU. Herein, we explore mammalian WDR75 molecular evolution, 3D structure, and phylogeny in light of its essential role in the SSU processome. We predict to find the footprint of purifying selection, especially at sites that are essential for proper ribosome assembly. In our comparison of 70 mammalian WDR75 sequences, we found ~25% of sites with significant purifying selection and no evidence of positive selection. Purifying selection was ~5x stronger for sites folding into beta-sheets than those predicted to be coils. Phylogenetic analysis validated expected mammalian relationships and uncovered an unusually long branch leading to mouse-eared bats, exhibiting 18x more substitutions per site than the average mammalian substitution rate. In testing for molecular evolution among branches, we found no evidence for purifying selection along any individual branches, but unexpectedly detected significant diversifying selection solely among African great apes.

A configurational path study of adolescents’ intention to participate in ice and snow sports based on the TPB and NAM frameworks

The association between different insulin resistance indexes and bone health in the elderly

The triglyceride-glucose (TyG) index and its related indexes (TyG-BMI, TyG-WC, TyG-WHtR) are effective markers for screening metabolic diseases like insulin resistance (IR). However, few studies have explored the relationship between the TyG and its related indexes with bone density (BMD), osteopenia, and osteoporosis. This is a cross-sectional study that involved 1,303 adults aged 50 years and above from the National Health and Nutrition Examination Survey 2007–2010, and 2013–2014. In the multivariable-adjusted model, linear regression analysis and logistic regression analysis demonstrated that TyG and its related indexes have a significant positive correlation with BMD and a negative correlation with osteopenia/osteoporosis in the femoral neck, lumbar spine, and total hip region. Trend analysis further confirms these associations (p < 0.05). Restricted cubic spline analysis showed a nonlinear relationship between these indexes with BMD and osteopenia/osteoporosis. Sensitivity analyses further confirmed the robustness of these associations. This study reveals the significant and complex correlation between the TyG and its related indexes with BMD and osteoporosis, indicating the potential link between IR and bone health. The TyG and related indexes offer a new perspective for the diagnosis, prevention, and treatment of osteoporosis.

Detecting glaucoma worsening using optical coherence tomography derived visual field estimates

Comparative genomic profiling of SLC26A4-expressing cells in the inner ear and other organs

Pendred syndrome and autosomal recessive non-syndromic hearing loss, type 4 (DFNB4), are associated with mutations in SLC26A4 that encodes the anion transporter SLC26A4 (pendrin). SLC26A4 is expressed in mitochondria-rich cells of the endolymphatic sac, spindle and root cells in the cochlear lateral wall, transitional cells in the vestibular organs, follicular cells in the thyroid, and type B intercalated cells in the kidney. This study aimed to assess the gene profiles of murine Slc26a4-expressing cells to better understand the regulatory mechanisms and functions of SLC26A4. Publicly available murine single-cell or single-nucleus RNA-sequencing (RNA-seq) datasets from the endolymphatic sac, cochlear lateral wall, utricle, kidney, airway, epididymis, and salivary glands were collected. After quality control, principal component analysis and clustering, distinct cell populations were identified, and differentially expressed genes (DEGs) were analyzed. The datasets were integrated for comparison across multiple tissues and organs. The results revealed no shared genetic profile among inner ear Slc26a4-expressing cells, with Slc26a4 being the only shared DEG, suggesting that regulatory genes may include low expression transcripts, splicing variants, or long non-coding RNAs undetectable by single-cell analysis. Comparative analysis within the ionocyte family identified distinct DEGs such as Insrr and Hmx2 in Slc26a4-expressing cells from the endolymphatic sac and kidneys, potentially significant in ion homeostasis and SLC26A4 regulation. This study highlights the specificity and complexity of SLC26A4 expression and highlights the challenges and limitations of single-cell analysis. Future research should address regulatory elements such as low-expression genes, splicing variants, and non-coding RNAs to enhance our understanding of SLC26A4 regulation across various cellular contexts.

Targeted cancer treatment using a novel EGFR-specific Fc-fusion peptide based on GE11 peptide

Effect of 433 MHz double-slot microwave antennas for double-zone ablation in ex vivo swine liver experiment

Purpose To evaluate the effects of axial length and slot-to-slot distance of double-slot microwave antenna (DSMA) with frequency of 433 MHz on the size and shape of ablation zones created under different input microwave powers. Materials and methods The design of double slot microwave antennas (DSMAs) with axial lengths (70 mm, 30 mm) and slot-to-slot distance (49 mm, 10 mm) were optimized by numerical simulation and ex vivo liver experiments. Finite-element method simulations and forty ablations of swine liver were employed to obtain the temperature distributions within liver tissue using DSMAs at the 433 MHz operating frequency in a range of heating powers (20, 30, 40 and 50W) for 600 s. The dependence of the effectiveness of MWA on the axial length and slot-to-slot distance of antenna as well as the input power was further evaluated by analyzing morphologic characteristics of ablated zone. Results Two-zone ablation was achieved by two types of double-slot antennas in our study with frequency of 433 MHz, and the observed shapes of ex vivo experimental ablation zones were in good agreement with patterns predicted by simulation models. The ablation zone exhibited a ‘gourd’ shape after the treatment using the antenna with longer axial length and slot-to-slot distance, while the short antenna caused a guitar-shape ablation in liver tissue after MWA. Conclusion The dedicated design of our DSMAs with a frequency of 433 MHz could enable new ablation shapes with controllable dimensions, which can be applied to the clinical treatment of MWA for gourd-shaped liver tumors and other long-shaped tumors. Furthermore, research can be conducted on how to design the antenna as flexible and use it for the treatment of pulmonary nodules or varicose veins.

Evolution of pathogenic Escherichia coli harboring the transmissible locus of stress tolerance: from food sources to clinical environments

The layering construction of the three-dimensional (3D) geological model for Wudalianchi volcanic area, Northeast China

Establishment of geological model in volcanic area is challenging owing to lack of borehole data and the effect of volcanic activity on rock distribution. Taking full advantage of the existing complete volcanic landforms and recognized for seven eruptive cycles in Wudalianchi volcanic area, here we apply a layered approach to build geological models for meeting rapid development of agriculture and understanding the evolution of regional geological structure. Based on the volcanic eruption cycle, the stratas in Wudalianchi volcanic area are divided into four layers. UGrid (unstructured grid in GMS) is used combining with DEM data to hierarchically build 3D geological structure model of volcanic area, which realize the visualization of regional stratigraphic distribution, and the reliability of the model is verified by the formation mechanisms of different types springs. The stratified modeling provides a scientific and effective mean for the reconstruction of geological structure in volcanic areas where the data are short and the stratigraphic distribution is complex. The 3D geological structure model established can lay a foundation for the prediction, evaluation and sustainable use of regional groundwater, geothermy, mineral water and mineral mud resources.

The impact of flatfeet on the correlation between functional movement scores, balance, agility, and core muscle strength in young females: a cross-sectional study

A study of high-speed train delays and relevant propagation influence characteristics

Faced with the complex and diversified disturbances of abnormal events in high-speed railway, studying the characteristics of delay scenario parameters and related propagation influence laws plays a fundamental role in analyzing the applicability of operation plans, formulating train operation adjustments, and evaluating the operation decision plans, as well as providing practical data support for the establishment of theoretical models. Based on the mechanical analysis of the primary and knock-on delays, this paper combines the daily safety information data and timetable data, and takes into account the modified primary delays upon speed loss, by taking the high-speed railway network in the Yangtze River Delta region as an example. On this basis, this paper further studies the statistical distribution of primary delay duration, event location distribution, emergent measures distribution, occurrence time and event cause distribution, and other characteristics. Based on this research, the distribution characteristics of the influenced train number and the cumulative delay under kinds of disturbances have been discussed, where the effects of running redundancy, propagation rate, and related parameters on delay propagation have got quantitative analysis. Research shows that the number of abnormal event samples distributed in the sections is 1.53 times that of stations. Based on the Fuzzy C-Means clustering results, abnormal events in the section are more likely to propagate than abnormal events at stations. For trains experiencing primary delay, the relationship between the maximum cumulative train delay and primary delay mostly obey a power-law distribution and the values of R2 are all greater than 0.65, indicating a high correlation at the theoretical level.

Retraction Note: Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

Drosophila Topoisomerase 3β binds to mRNAs in vivo, contributes to their localization and stability, and counteracts premature aging

Topoisomerase 3β (Top3β) works not only on DNA but also on RNA. We isolated and identified the naturally cross-linked RNA targets of Drosophila Top3β from an early embryonic stage that contains almost exclusively maternal mRNAs. Favorite targets were long RNAs, particularly with long 3’UTRs, and RNAs that become localized in large cells. Top3β lacking only the hydroxyl group that makes the covalent bond to the RNA, did not allow normal expression and localization of Top3β mRNA targets or their protein products, demonstrating the importance of the enzymatic activity of Top3 β for optimized gene expression. Top3β is not essential for development to the adult stage but to maintain the morphology of the adult neuromuscular junction and to prevent premature loss of coordinated movement and aging. Alterations in human Top3β have been associated with several neurological diseases and cancers. The homologs of genes and (pre)mRNAs mis-expressed in these conditions show the same characteristics identified in the Drosophila Top3β targets, suggesting that Drosophila could model human Top3β. An in vivo test of this model showed that the enzymatic activity of Top3β reduces the neurodegeneration caused by the cytotoxic human (G4C2)49 RNA. Top3β supports normal gene expression, particularly of long and complex transcripts that must be transported and translationally controlled. These RNAs encode large cytoskeletal, cortical, and membrane proteins that are particularly important in large and long cells like motoneurons. Their reduced expression in the mutant seems to stress the cells, increasing the chances of developing neurodegenerative diseases.

A symmetry-based mechanism for perceptual grouping in preverbal infants

Single-unit activity in the anterior claustrum during memory retrieval after trace fear conditioning

We have recently identified a group of claustral neurons that continuously maintain information associated with a fear-conditioned stimulus (CS) for at least tens of seconds, even after the CS has ceased. This “online state” refers to the persistent maintenance of threat-associated information, enabling it to be actively processed even after the threat has terminated. This state may involve reciprocal interactions of the claustral neurons with brain regions involved in decision-making, motor preparation, and adaptive behavioral responses. If these claustral neurons truly encode the online state, their function should remain independent of the modality of the threat stimulus or the specific defensive behavior exhibited. In this study, we used a tone cue and monitored freezing behavior in trace conditioning, in contrast to the light cue and escape behavior used in our recent study. During the retrieval test of trace conditioning, a subset of rostral-to-striatum claustrum (rsCla) neurons exhibited sustained activity in response to the CS, particularly during the trace interval. Importantly, we found a positive correlation between the activity of rsCla neurons and the magnitude of freezing during the trace interval, when intervals without freezing were excluded. Thus, this subset of rsCla neurons appears to exhibit the characteristics of ‘online neurons’ during memory retrieval following trace conditioning.