Browse Articles

Answering the Call — Sustaining Equity Efforts in the Face of Regression

Preliminary survey of biofilm forming, antibiotic resistant Escherichia coli in fishes from land based aquaculture systems and open water bodies in Bangladesh

Structural basis of Nipah virus RNA synthesis

Abstract Nipah virus (NiV) is a non-segmented negative-strand RNA virus (nsNSV) with high pandemic potential, as it frequently causes zoonotic outbreaks and can be transmitted from human to human. Its RNA-dependent RNA polymerase (RdRp) complex, consisting of the L and P proteins, carries out viral genome replication and transcription and is therefore an attractive drug target. Here, we report cryo-EM structures of the NiV polymerase complex in the apo and in an early elongation state with RNA and incoming substrate bound. The structure of the apo enzyme reveals the architecture of the NiV L-P complex, which shows a high degree of similarity to other nsNSV polymerase complexes. The structure of the RNA-bound NiV L-P complex shows how the enzyme interacts with template and product RNA during early RNA synthesis and how nucleoside triphosphates are bound in the active site. Comparisons show that RNA binding leads to rearrangements of key elements in the RdRp core and to ordering of the flexible C-terminal domains of NiV L required for RNA capping. Taken together, these results reveal the first structural snapshots of an actively elongating nsNSV L-P complex and provide insights into the mechanisms of genome replication and transcription by NiV and related viruses.

Genetic Medicine for Danon Disease

Effects of gradual deformation of identical and nonidentical, rotated and nonrotated U-shaped subwavelength resonators in few-layer metasurfaces

Forecasting the eddying ocean with a deep neural network

Unveiling the Unforeseen

Author Correction: The tree-ring width and interval trend values as indicators of sensitivity to temperature and precipitation in different provenances of European larch

Publisher Correction: Numerous rRNA molecules form the apicomplexan mitoribosome via repurposed protein and RNA elements

Phase 1 Study of AAV9.LAMP2B Gene Therapy in Danon Disease

Leveraging swin transformer with ensemble of deep learning model for cervical cancer screening using colposcopy images

Frequency transfer and inverse design for metasurface under multi-physics coupling by Euler latent dynamic and data-analytical regularizations

VITT-like Monoclonal Gammopathy of Thrombotic Significance

Kinetic analyses for solid-state phase transition of metastable amorphous-AlOx (2.5 < x ≤ 3.0) nanostructures into crystalline alumina polymorphs

Aberrant activation of chromosome asynapsis checkpoint triggers oocyte elimination

Bankruptcy, Genetic Information, and Privacy — Selling Personal Information

A functional approach to model intrinsic capacity in ageing trajectories

Tracking HIV persistence across T cell lineages during early ART-treated HIV-1-infection using a reservoir-marking humanized mouse model

PCI in Patients Undergoing TAVI

Synergistic effects of cold atmospheric plasma and doxorubicin on melanoma: A systematic review and meta-analysis

Abstract Melanoma is responsible for the majority of skin cancer deaths, but there are ways to combat this deadly disease. One method is using anti-neoplastic agents, such as Doxorubicin (DOX). Unfortunately, DOX can be toxic and may lead to drug resistance. However, researchers are excited about the potential of Cold Atmospheric Plasma (CAP) treatment cancer cells and overcome drug resistance selectively. To better understand the effectiveness of the combination of CAP and DOX on melanoma cell viability, cytotoxicity, and cell death, we conducted a comprehensive evaluation and meta-analysis in this study. 41 studies out of 121 met our inclusion criteria. The pooled analysis found that CAP and DOX combination had a significant effect on cell viability (ES = 6.75, 95% CI 1.65 to 11.85, and I2 = 71%) and cytotoxicity (ES = 11.71, 95% CI 3.69 to 19.73, and I2 = 56%). however, no statistically significant association was found between cell death with combination treatment. Our studies have confirmed that the combined treatment of CAP and DOX has a synergistic effect on reducing cell viability and increasing cytotoxicity in melanoma cells. These results can assist researchers in selecting more effective treatment methods to address melanoma.