Browse Articles
Discover research articles across all indexed journals
How the US tech industry is shaping the transition to green energy
Precise modelling of mitochondrial diseases using optimized mitoBEs
Author Correction: The biodegradable polymer poly(butylene succinate-co-adipate) modulates the community structures of actively growing bacteria in rotifer culture water
Experience of implementing metagenomic next-generation sequencing in patients with suspected pulmonary infection in clinical practice
What is stopping a complete switch to clean energy?
Factors influencing the agricultural extension model sites in Iran
Data-driven analysis for the evaluation of cortical mechanics of non-adherent cells
Publisher Correction: Study of the mechanical compression properties of Rosa sterilis S.D. Shi based on FEM
Study on the bearing capacity optimization and performance of photovoltaic support in desert sand and gravel area based on bionics
A fusion method of infrared and visible images based on visual salience difference
Effect of antecedent B-cell depletion therapy for patients hospitalized with COVID-19 within a single health system: a propensity score analysis
Coracoid approach brachial plexus block combined with posterior suprascapular nerve block provides analgesia for shoulder arthroscopy: a randomized controlled trial
Author Correction: Clinical characteristics and prognosis of patients treated as invasive pulmonary aspergillosis outside of severe immunosuppression
Anatomical characterisation of somatostatin-expressing neurons belonging to the anterolateral system
Abstract Anterolateral system (ALS) spinal projection neurons are essential for pain perception. However, these cells are heterogeneous, and there has been extensive debate about the roles of ALS populations in the different pain dimensions. We recently performed single-nucleus RNA sequencing on a developmentally-defined subset of ALS neurons, and identified 5 transcriptomic populations. One of these, ALS4, consists of cells that express Sst , the gene coding for somatostatin, and we reported that these were located in the lateral part of lamina V. Here we use a Sst Cre mouse line to characterise these cells and define their axonal projections. We find that their axons ascend mainly on the ipsilateral side, giving off collaterals throughout their course in the spinal cord. They target various brainstem nuclei, including the parabrachial internal lateral nucleus, and the posterior triangular and medial dorsal thalamic nuclei. We also show that in the L4 segment Sst is expressed by ~ 75% of ALS neurons in lateral lamina V and that there are around 120 Sst -positive lateral lamina V cells on each side. Our findings indicate that this is a relatively large population, and based on projection targets we conclude that they are likely to contribute to the affective-motivational dimension of pain.