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Design, synthesis and pharmacological evaluation of 1,4-naphthoquinone- 1,2,3-triazole hybrids as new anticancer agents with multi-kinase inhibitory activity

Redox cycling of sulfur via microbes in soil boosts the bioavailability of nutrients to Brassica napus

Deficiency of sulfur (S) has been recognized for both dry and wetland plants as a significant growth limiting factor. S-oxidizers enhance the oxidation rate of S and increase sulfate (SO4 − 2) production by making them available in critical growth stages, resulting in higher plant growth and yield. A two-phase study was undertaken to assess the effectiveness of microbial redox reactions on soil nutrient bioavailability and crop growth. In the first phase isolation of S bacteria was carried out while in the second phase, a pot experiment was conducted and these bacteria were evaluated as a nutrient source along with different ratios of NPK fertilizer by growing canola (Brassica napus) as a test crop. Treatment combinations were, viz., Control (no inoculum), ½ NPK fertilizer (50, 30, and 25 kg ha−1), Full NPK fertilizer (100, 60, and 50 kg ha−1), ½ NPK+SOB, ½  NPK+SRB and ½ NPK+SOB+SRB. Sulfur oxidizing bacteria (SOB) strains were isolated from samples and then screened based on pH reduction (in thiosulphate broth). Sulfur-reducing bacteria (SRB) are characterized by their anaerobic energy metabolism based on the dissimilatory reduction of SO4−2 to hydrogen sulfide (H2S) or S ° to be useful for SOB. Among different bacterial consortiums, the best results for nutrient bioavailability (macro- and micronutrient) in soil and plant the treatment ½ NPK+SOB+SRB compared to full NPK increased soil N, P, K, and SO₄ by 15.9%, 38%, 2.0%, and 72%, respectively. In plants, it boosted N, K, and SO₄ by 7.7%, 31%, and 239%, respectively. The greatest pH reduction (4%) was observed with ½ NPK+SOB relative to full NPK. This study demonstrates that microbial inoculation along with ½ NPK increases the bioavailability of macro- and micronutrients for crops.

Caveolin 1 and 2 enhance the proliferative capacity of BCAM-positive corneal progenitors

Abstract Caveolin (CAV) 1 and 2 are integral membrane proteins that constitute major components of small membrane pouches termed caveolae. While several functions have been described in other tissues, the roles of CAV1 and CAV2 in the ocular surface have remained unknown. In the current study, we investigated the expression and function of CAV1 and CAV2 in the human cornea. We found CAV1 and CAV2 to be preferentially expressed by proliferative Basal Cell Adhesion Molecule (BCAM)-positive progenitor cells along the entire limbal and corneal basal epithelial layer. Functional gene knockdown studies reveal that BCAM, BCAM co-expressed Laminin α5 (LAMA5) and Laminin α3 (LAMA3) regulate expression of CAV2. Mechanistically, we demonstrate that CAV1 and CAV2 contribute to enhanced BCAM-positive cell proliferation through regulation of Fibroblast Growth Factor Receptor 2 (FGFR2) cell surface expression. In aggregate, our study identifies specific expression of CAV1 and CAV2 in BCAM-positive corneal basal epithelial cells and uncovers a novel CAV1/CAV2-dependent mechanism of corneal progenitor cell proliferation, with potential implications for therapeutic enhancement of corneal regeneration.

Adaptation to acidic conditions that mimic the tumor microenvironment, downregulates miR-193b-3p, and induces EMT via TGFβ2 in A549 cells

The acidic tumor microenvironment plays a critical role in the malignant transformation of cancer cells. One mechanism underlying this transformation involves epithelial-mesenchymal transition (EMT). This is induced by prolonged exposure to acidic conditions. EMT is an essential process in cancer progression, with Transforming Growth Factor Beta (TGF-β) playing a central role in its induction. However, little was known about the factors regulating TGF-β under acidic conditions. This study aimed to elucidate the mechanism of EMT under acidic conditions and identify novel therapeutic targets to inhibit cancer cell migration and metastasis. Focusing on lung cancer, we explored microRNAs associated with EMT that were differentially expressed under acidic conditions in A549 cells and identified miR-193b-3p as a novel candidate. Under acidic conditions, miR-193b-3p expression decreased around days 3–14. Downregulation of miR-193b-3p promoted increased TGFβ2 expression, resulting in EMT changes in A549 cells. Our study suggests that the interaction between miR-193b-3p, TGFβ2, and the acidic tumor microenvironment promotes cancer EMT change. Understanding these interactions may not only enhance our biological comprehension of cancer, but also pave the way for the development of targeted therapies to inhibit cancer metastasis.

Antimicrobial activities of biosynthesized nanosilver using Musa paradisiaca and Citrus sinensis peel extracts against major human and plant pathogens

Virtual voyage: Unveiling the ‘3I’ influence on student learning

This study delves into the multifaceted “3I” technological attributes of metaverse classrooms—namely perceived immersion, perceived interaction, and perceived imagination—and their interplay with psychological imagery and representational ability. This investigation seeks to elucidate the nexus between these constructs and their collective influence on students’ learning outcomes. To achieve this, an integrative conceptual model has been meticulously constructed, encapsulating the dynamic interrelationships between the “3I” characteristics and the pivotal cognitive factors of psychological imagery and representational ability. Employing a Partial Least Squares Structural Equation Modeling (PLS-SEM) approach, an empirical inquiry was conducted on a dataset comprising 930 meticulously validated questionnaires. The analysis yielded significant findings: perceived immersion and perceived interaction were found to exert a direct and positive influence on learning outcomes. Furthermore, psychological imagery emerged as a central mediator in the pathway from metaverse classroom characteristics to learning outcomes. Representational ability was identified to have dual roles, exerting both positive and negative moderating effects on the relationship between perceived imagination and learning outcomes, as well as between psychological imagery and learning outcomes.The study’s empirical evidence offers substantial theoretical and empirical validation for the practical application and strategic refinement of metaverse-based educational practices. It underscores the imperative to account for individual differences and to foster immersive and interactive learning environments. Additionally, the study highlights the untapped potential of perceived imagination in enhancing learning outcomes, suggesting a rich vein for future research.

Research on digital infrastructure construction empowering new quality productivity

Robust adaptive control with dung beetle optimization algorithm and disturbance observer for load displacement tracking of shock absorber damper test bench

Shock absorbers are indispensable components utilized for vibration mitigation in various fields, including construction, bridges, wind power, and pipelines. The shock absorber test bench serves as an essential apparatus for evaluating the dynamic and static characteristics of these absorbers. To address the significant tracking errors between actual and expected displacements during displacement loading on shock absorber damper test benches, a novel control strategy is proposed. This strategy incorporates a robust adaptive controller (RAC) enhanced by a dung beetle optimization (DBO) algorithm and a disturbance observer (DO). The dung beetle optimization algorithm is specifically designed to iteratively optimize the control parameters of the robust adaptive controller. Concurrently, a disturbance observer is implemented to accurately estimate external disturbances and perform feedforward compensation. A mathematical model of the electro-hydraulic servo control system of the test bench is established, and the stability of the proposed controller is rigorously verified using Lyapunov theory. To simulate and analyze the control method for the electro-hydraulic servo system of the test bench, a joint simulation model integrating Simulink and AEMSim is constructed. The performance of the proposed robust adaptive controller with DBO and DO is compared against an unoptimized robust adaptive controller and a traditional PID controller in terms of load displacement tracking. Simulation results demonstrate that the control method proposed in this study significantly outperforms other controllers in enhancing position tracking accuracy and improving system robustness. Furthermore, experimental verification was carried out on the proposed control strategy, compared with unoptimized robust adaptive control, the maximum displacement tracking error was reduced by 54.8%, and the response speed was improved by 36.3%, compared with traditional PID control, the minimum displacement tracking error was reduced by 67.4%, and the response speed was improved by 47.7%. The results confirmed the superior performance of the proposed control method.

Natural language processing of electronic medical records identifies cardioprotective agents for anthracycline induced cardiotoxicity

Examining the relationship between reproductive empowerment and contraceptive self-injection: Tackling the endogeneity problem

Background Self-care interventions, including contraceptive self-injectables such as subcutaneous depot medroxyprogesterone acetate (DMPA-SC), are hypothesized to be empowering to users. It is also believed that those who are empowered are more likely to use self-care. Though critical for ensuring equity of these interventions, evidence for the relationship between empowerment and contraceptive self-care is scant. However, studying this relationship is challenging. In addition to the potential reversed causality between these two constructs, empowerment is determined by similar factors as the motivation for using self-care, contributing to an endogeneity problem. If not addressed, endogeneity can lead to incorrect causal assertions. Methods Using data from a study of 400 women in Addis Ababa, Ethiopia who opted to self-inject DMPA-SC, we assessed the directionality between the two constructs. First, we assessed the change in empowerment after participants’ first self-injection. Second, we assessed the effect of empowerment on potential future use of self-injection. To address potential endogeneity, we identified instrumental variables of empowerment and then applied a two-stage regression approach to predict desire to continue self-injecting at follow-up with an instrument for empowerment, controlling for other variables. Results Empowerment scores among the 343 women who were followed-up were high and did not significantly change from baseline to endline. Most women (78%) wanted to continue self-injecting. The following variables were identified and used as instruments: religion, employment status and post-secondary school attendance. The final model did not identify a significant relationship between desire to continue self-injecting and empowerment. The test of exogeneity was marginally significant (p = 0.08). Conclusions We did not find evidence of a significant relationship between reproductive empowerment and desire to continue self-injecting. Though there are limitations to this secondary data analysis, we recommend future research investigate this relationship using the methodology demonstrated to address endogeneity inherent in answering this critical question about self-care interventions.

Method for EEG signal recognition based on multi-domain feature fusion and optimization of multi-kernel extreme learning machine

In vivo biocompatibility and long-term durability of nanofibrillated cellulose as a urethral bulking agent in rats and Beagle dogs

Background Cystoscopy-assisted submucosal injections of urethral bulking agents offer a safe and efficient alternative to surgery for treating urinary incontinence in both dogs and women. To address the concern of their transient therapeutic effect, a preclinical study evaluating the biocompatibility, safety, and durability of nanofibrillated cellulose as a bulking agent was designed. Plant-based nanofibrillated cellulose is considered renewable, biocompatible, and non-degradable in vivo. To the best of our knowledge, no studies of nanofibrillated cellulose injected into the urethral wall of experimental animals have been published to date. Methods After assessing the rheological behavior of nanofibrillated cellulose, a biocompatibility study with 50 rats and a durability study with two Beagle dogs were conducted. In anesthesized rats, deposits of either nanofibrillated cellulose or sodium chloride as an inert control were injected into the urethral wall via a caudal laparotomy. The rats were euthanized for histopathological assessment after 7, 30, and 90 days. In dogs, cystoscopy-assisted injections of nanofibrillated cellulose were followed with magnetic resonance imaging at 14 days and at 2, 3, 6, and 12 months. Results The rheological studies demonstrated a gel-like behavior under a wide range of shear stress. Nanofibrillated cellulose induced a moderate host tissue response according to the EN ISO 10993-6 standard, consisting primarily of macrophages, foreign body giant cells, lymphocytes, and plasma cells. No significant difference was observed in the tissue response at different time points. In dogs, the bulking agent was visible in 4/5 (80%) injection sites on magnetic resonance imaging at 12 months post-injection. No signs of migration, abscess formation or any major or long-term complications were observed. Conclusions Nanofibrillated cellulose maintains a chronic but stable and tolerable inflammatory response for up to 90 days in the urethral wall of rats. Durability in the urethral wall of dogs indicates a potential long-term effect.

Association between left atrial function and pulmonary vein stump thrombus after left upper lobectomy: insights from cine-MRI

Developing a localized resilience assessment framework for historical districts: A case study of Yazd, Iran

Growing natural and man-made disasters necessitate enhanced resilience in urban historical districts, vital for cultural heritage and tourism. This study aims to develop a localized assessment framework tailored to the unique characteristics of Yazd, Iran, a UNESCO World Heritage site known for its ancient architecture and cultural significance. By adapting and downscaling indicators from established DROP and BRIC models, we evaluated resilience across seven key dimensions and 17 criteria. Using advanced multi-criteria decision-making methods, including Delphi, Fuzzy DEMATEL, Fuzzy ANP, and VIKOR, we prioritized and ranked the historical districts based on their resilience scores. The results revealed that the social dimension and housing infrastructure are the most crucial factors for resilience. Environmental and institutional dimensions, while important, were found to be less critical in comparison. The VIKOR analysis identified specific districts with lower adaptability, requiring targeted interventions. These findings provide valuable information for policymakers and urban planners, offering a robust framework for enhancing urban historical district resilience. This study provides a context-specific approach to resilience assessment, emphasizing the need for tailored strategies to preserve and strengthen the resilience of culturally significant urban areas.

Knockdown of LncRNA MEG3 promotes damage of vascular endothelial cells induced by vibration

Spatiotemporal distribution characteristics of Nanjing place names—Based on data mining of Tang-Song poetry and online travelogues

Tang-Song poetry, a distinguished element of China’s traditional cultural heritage, is intricately linked with the historical and cultural development of Chinese cities. This paper uses Nanjing as a case study and applies digital humanities techniques to analyze and compare the spatiotemporal distribution of place names found in Tang-Song poetry with those in online travel narratives. The aim is to uncover key factors that have influenced the cultural continuity of these historical cities and their relevance today. Findings indicate that: (1) Locations mentioned in Tang and Song poetry show significant spatial differentiation, with urban areas displaying a clustered distribution and suburbs showing scattered hotspots. (2) The number of locations referenced in Song poetry increased significantly compared to Tang poetry, suggesting that Nanjing’s economic growth heightened the city’s appeal and inspired more literary output. (3) Song Dynasty poetry reflects a shift toward more neutral and negative emotions, with a marked decrease in positive expressions. This rise in negative sentiment can be traced to the decline in national strength from the Tang to the Song Dynasty, amplifying Nanjing’s role as a place of reflection and mourning. (4) Nanjing’s cultural hotspots, such as Xuanwu Lake and the Zhongshan Scenic Area, feature prominently in both Tang-Song poetry and modern travelogues. This study contributes to research in literary geography and literary tourism at the urban spatial level, offering fresh insights into the cultural legacy of historical cities.

Bayesian Gower agreement for categorical data

Factors involving in healthcare professionals’ decision-making process regarding the use of restrictive care practices in adult mental health inpatient units: A protocol for an umbrella review

Introduction The use of restrictive care practices, such as physical restraint, seclusion, and chemical restraint, remains common practices, yet controversial aspect of mental health care, particularly in inpatient settings. Variations in legal definitions and protocols guiding the implementation of these practices often create legal and ethical dilemmas for professionals when deciding whether to implement such practices in certain circumstances. This leads them to rely on subjective decision-making, which is further shaped by epistemic disparities stemming from differences in knowledge, perspectives, and experiences that impact professionals’ understanding and implementation of restrictive practices. Aims This is a study protocol for an upcoming umbrella review aiming to identify and analyse the thematic elements that affect healthcare professionals’ decision-making regarding the uimplementations of restrictive care practices in adult mental health inpatient settings. Methods This protocol outlines the approach for an umbrella review. The review will conduct a systematic literature search of electronic databases (Scopus, CINAHL, Embase, OVID, PsycINFO, Cochrane Reviews, and Web of Science) using comprehensive search strings, as well as a manual review of reference lists from included reviews. Only review studies will be considered. Textual data discussing issues related to professionals’ decision-making regarding the use of restrictive care practices will be extracted from each study, coded, and analysed using inductive content analysis. This protocol has been reported according to the PRISMA-P Checklist. Discussion The findings from this review will provide valuable insights into the key factors influencing the implementation of restrictive practices in adult mental health inpatient settings. The factors identified in this umbrella review will be applied in clinical practices to guide healthcare practitioners in considering and navigating various contexts when deciding how and in which situations restrictive care practices should be implemented. This contributes to the current global effort in promoting more patient-centered, transparent, evidence-based and accountable practices, while minimizing restrictive interventions. It will also inform future training, practice, and policy interventions aiming at reducing the use of restrictive measures in adult mental health settings. Protocol registration: This protocol has been registered in PROSPERO (CRD-42024581848).

Prognostic value of IFI27 in HNSCC and functional analysis under ALKBH5 regulation

Seasonal dynamics and adaptation strategies of krummholz forming Rhododendron campanulatum to water availability at high-altitude Himalayan treeline environments

This study investigates the water relations mechanism of Rhododendron campanulatum (D. Don) in treeline areas of the Himalayan region, emphasizing its vital role in ecosystem dynamics. The species, commonly found as krummholz forms at treeline ecotones, exhibits notable population advancement 1.4 m/yr, which could lead to the densification of the ecotone in the future. Seasonal measurements of soil moisture at depths of 15cm, 30cm, and 45cm, as well as pre-dawn (ΨPD) and mid-day (ΨMD) water potentials, and leaf conductance in the forenoon (gwAM) and afternoon (gwPM), were conducted for both trees and seedlings. Significant variations were observed among sites, seasons, and years across various parameters, including soil moisture and water potential components. The study underscores the most negative water potentials (Ψ) during winters, with ΨPD reaching -0.88 MPa for trees and -1.43 MPa for seedlings. Seasonal changes in water potential (ΔΨ) ranged from 0.11 to 0.82 MPa for trees and 0.20 to 0.90 MPa for seedlings. Osmotic potential at full turgor (OPFull) declined from winter to summer, ranging from -0.74 to -1.76 MPa in trees and -0.45 to -1.60 MPa in seedlings. Relative water content (RWC%) of seedlings varied between 68.00% and 87.40%. Leaf conductance ranged from 19.50 to 329.68 m mol m-2 sec-1 in trees and 43.60 to 331.40 m mol m-2 sec-1 in seedlings. The study highlights the adaptation of R. campanulatum and other broad-leaved evergreen species to high-altitude climatic irregularities, emphasizing the crucial role of snowmelt and monsoon rains in mitigating water stress. Osmotic adjustment and high relative water content enable R. campanulatum to cope with environmental changes, maintaining water availability for photosynthesis.