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Image encryption scheme based on thorp shuffle and pseudo dequeue
The glycolytic enzyme PKM2 regulates inflammatory osteoclastogenesis by modulating STAT3 phosphorylation
Voluntarily wheel running protects doxorubicin-induced kidney injury by inhibiting oxidative stress through mitochondrial function
Background Doxorubicin (DOX) has a broad anticancer spectrum and precise anticancer effects, but its clinical application is limited by severe multiorgan toxicity, among which nephrotoxicity is one of the main adverse reactions. In this study, the protective effect of voluntary wheel running on nephrotoxicity induced by DOX was observed, and its mechanism was initially discussed. Methods Forty male C57BL/6 mice were randomly divided into a control group (CTR), a voluntary wheel running group (EX), a doxorubicin model group (DOX) and a doxorubicin combined with voluntary wheel running group (COM). After 2 weeks of exercise, the mice were sacrificed. Serum creatinine (CREA), urea nitrogen (BUN), uric acid (UA), carbon dioxide combining power (CO2-CP), renal tissue apoptosis, oxidative stress and mitochondrial function indicators were assessed. Results Compared with those in the DOX group, the concentrations of CREA, BUN and UA decreased, the number of TUNEL-positive cells in kidney tissue decreased, the expression of antiapoptotic proteins increased, and the expression of proapoptotic proteins decreased in the COM group. In addition, the COM can reduce the ROS and MDA contents in kidney tissue, reduce peroxide accumulation and alleviate mitochondrial respiratory chain damage caused by DOX. Conclusions Voluntary wheel running can improve the mitochondrial function of renal cells and reduce oxidative stress damage, thus playing a protective role against nephrotoxicity caused by DOX. This study provides a new way to reduce the adverse reactions to chemotherapy in combination with the application of chemical drugs.
Optimizing lipase production by Bacillus subtilis on cheese whey and evaluating its antimicrobial, antibiofilm, anti virulence and biosafety properties
Abstract This study optimized lipase production using cheese whey, biofilm inhibition, and antibacterial efficacy of Bacillus subtilis (DSM 1088)derived lipase against Staphylococcus aureus (ATCC 6538). Peak lipase activity, growth rate, and inhibitory potential were observed at 48 h and 30 °C. Using Plackett-Burman and Central Composite Designs (PBD and CCD), whey, peptone, and agitation speed were identified as significant factors, achieving optimal lipase activity of 1314 U/mL and an inhibitory zone diameter (IZD) of 48 mm against S. aureus. Partial purification through ammonium sulfate precipitation and dialysis increased partial purified lipase (PPL) activity by twofold and fivefold, respectively. PPL exhibited effective bactericidal properties with a minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of 1/8 and 1/16, confirming a bactericidal effect (MIC/MBC ratio ≤ 2). Biofilm inhibition assays demonstrated 95% biofilm reduction at 80 µg/mL PPL, with SEM imaging revealing significant biofilm matrix disruption. Time-kill assays showed concentration-dependent bactericidal action, while inhibition of hemolysin and protease activities (25–100%) indicated reduced S. aureus pathogenicity. Cytotoxicity assays on normal liver cells showed an IC50 > 300 µg/mL, indicating low toxicity. GC/MS analysis of oil waste before degradation identified predominantly oleic acid 3-hydroxypropyl ester and octadecane derivatives, while after degradation, it revealed enriched free fatty acids including myristic, palmitic, linoleic, and oleic acids, which could enhance antimicrobial efficacy. Molecular docking suggested that PPL inhibits essential bacterial enzymes (folic acid synthetase, RNA polymerase, DNA gyrase), potentially disrupting DNA synthesis and promoting cell death. These findings highlight B. subtilis-derived lipase as a promising bio-agent for combating biofilm-associated, drug-resistant pathogens with clinical and industrial applications.
A novel spider toxin as a selective antagonist of the Kv1 subfamily of voltage-gated potassium channels through gating modulation
Gamifying water crisis management: A serious game for drinking water contamination emergency response
This paper presents a serious game that simulates a water crisis triggered by the spill of an unregulated chemical. The game includes five stakeholder roles representing a chemical manufacturer, resident, water treatment plant, environmental agency, and health department, in addition to a facilitator role. The game seeks to provide players with practical experience of the communication and collaboration needed among different stakeholders to prepare for and respond to water contamination emergencies. Initial findings from game sessions with 41 participants suggest that frequent, proactive, and transparent communication can expedite the decision-making process and resolve the crisis more effectively. The game results also reveal challenges in inter-organizational coordination and communication, highlighting the need for training and standardized communication terminologies and protocols.
Double burden of malnutrition among under-five children in Eastern and Southern African countries
YibN, a bona fide interactor of the bacterial YidC insertase with effects on membrane protein insertion and membrane lipid production
Changes in attitudes of mental health care staff surrounding the implementation of peer support work: A qualitative study
Background The integration of Peer Support Workers (PSWs) in mental health settings may encounter resistance due to the attitudes of Mental Health Workers (MHWs). At the same time, PSWs may initiate changes in the attitudes, as well as in the larger institutional culture. This paper addresses the question of which changes happen and how they occur. Methods This work is part of the ImpPeer-Psy5 study, funded by the German Innovation Fund, which examines the nationwide implementation of PSWs in health insurance-funded mental health care. Data were derived from two qualitative sub-studies: QUAL1encompassed 57 problem-centered interviews conducted with PSWs, MHWs working together with PSWs, and service users; QUAL2 consisted of one focus group and five interviews only with MHWs. Qualitative data were analyzed using a modified thematic analysis adapted to a collaborative research approach. Results The main findings included that working with PSWs makes MHWs better integrate lived experience insights into their caregiving, pay more attention to life contexts, advance towards a more appreciative language, use diagnostic categories more flexibly, and show more openness towards their own affectedness. These changes are reported to be mediated by the simple presence of PSWs, their “role model” function for other staff, or more direct forms of feedback and critique. Conclusion Overall, staff attitudes changed as a result of reflexive processes induced by their interactions with PSWs. This poses a dilemma as changes in MHWs and larger institutions are expected to occur prior to the implementation of peer support work to facilitate their integration. Further research is needed to evaluate whether and how staff training can facilitate the implementation of PSWs by targeting MHWs’ attitudes.
Creep failure characteristics and damage creep model of red layer soft rock based on Perzyna viscoplastic theory
Interaction between CaV2.1 and Junctophilin3/4 depends on the II-III loop of CaV2.1 and on the α-helical region of Junctophilin3/4
Effects of hexavalent chromium on the biology of Steinernema feltiae: evaluating sublethal endpoints for ecotoxicity testing
Essential information about the effects of a pollutant on an ecosystem can be obtained by observing how it influences a bioindicator organism. Hexavalent chromium (Cr VI+) naturally occurs in Irish agricultural soils at levels of up to 250 mg/kg, which raises concerns about potential negative impacts on human health and the surrounding areas. This research aimed to assess the sublethal effect concentrations (up to 300 ppm) of Cr VI + on the entomopathogenic nematode (EPN) Steinernema feltiae focusing on endpoints such as nematode movement and host finding ability in contaminated sand and pathogenicity, percentage penetration, sex ratio and reproduction in Galleria mellonella. To achieve that, an Irish isolate of S. feltiae [strain SB 12 (1)], was used in all experiments. The attraction of nematodes to the insect host was tested using PVC tubes of various lengths, containing sand with various concentrations of Cr VI + (50-300 ppm in increments of 50). The replication was tenfold and the insect mortality was recorded at the end of the experiment. Results showed that there was a significant effect of Cr VI + on the pathogenicity, movement and host finding ability of the nematodes in contaminated sand, and on the percentage of penetration in an insect host. However, no significant effects among the studied Cr VI + concentrations were observed in S. feltiae reproduction in G. mellonella. Similarly, the presence of the toxicant (at low concentration of 12ppm) did not affect the growth of the nematode symbiotic bacteria in liquid and solid media (TSA and NBTA). Reproduction, unlike the other sublethal parameters tested, appeared not to be an optimal endpoint for assessing soil Cr VI + risk contamination. Overall, this study confirms the excellent potential of S. feltiae to be used as a suitable sentinel organism in assessing the risk of Cr VI + soil contamination especially in the contexts of agriculture and soil health.
Association between environmental stress factors, salivary cortisol level and dental caries in Egyptian preschool children: a case-control study
Abstract Dental caries remains the most common chronic disease in childhood. This study aimed to identify environmental stressors in children and estimate the association between salivary cortisol level as a stress biomarker and childhood caries. We conducted a case-control study involving 80 Egyptian children. The cases were 40 children with early childhood caries (ECC). The controls included 40 caries-free children. Data were collected from the parents or caregivers of the children using a questionnaire. The clinical examination included caries experience and an oral hygiene index. Saliva samples were collected and tested for unbounded cortisol levels. The results from the current study showed that the mean age for participants was 5.09 ± 0.66 years. Twenty-one of the children with ECC were girls. In multivariate regression, there is a significant association between ECC and elevated salivary cortisol levels (> 0.57 µg/dl) with an adjusted odds ratio (AOR) of 9.649 (p = 0.003), father smoking (AOR = 5.791, p = 0.030), sleeping with a bottle or food in the mouth (AOR = 8.481, p = 0.027), and poor oral hygiene evaluated through the Silness-Löe Plaque Index (AOR = 6.681, p = 0.001). As a conclusion, the study indicates that salivary cortisol is an independent stress biomarker in relation to early childhood caries. Salivary cortisol as a stress biomarker can help clinicians provide an additional assessment tool for identifying caries risk in children.
Substrate-specific effects point to the important role of Y361 as part of the YER motif in closing the binding pocket of OCT1
Correction: Rearing methods and life cycle characteristics of Chironomus sp. Florida (Chironomidae: Diptera): A rapid-developing species for laboratory studies
Tranexamic acid in ear surgery: enhancing surgical field visibility and reducing bleeding—a systematic review and meta analysis
Pseudomonas aeruginosa PfpI is a methylglyoxalase
A predictive model for left ventricular reverse remodeling after pharmacological therapy in children with recent-onset dilated cardiomyopathy
Background Pharmacological advances have improved pediatric dilated cardiomyopathy (DCM) prognosis, which manifests as left ventricular reverse remodeling (LVRR). However, significant inter-individual variability exists in therapeutic response. Identifying predictors is critical for individualizing management to inform device and transplant timing. Aim To develop a nomogram for predicting LVRR in pediatric DCM. Methods A retrospective analysis of 146 children hospitalized for DCM from January 2012 to June 2023. 55 exhibited LVRR. A nomogram predicting pediatric DCM-LVRR was developed using univariate analysis and logistic regression to select predictors. The nomogram was validated via bootstrapping and receiver operating characteristic curves for discrimination. Calibration was assessed with the Hosmer-Lemeshow test. Decision curve analysis evaluated performance and utility. Results Age, left ventricular end-diastolic dimension Z-score, and QRS interval were associated with the occurrence of LVRR. Discrimination was high (C-index 0.903) and internally validated on bootstrapping with 1000 repetitions (Adjusted C-index 0.895). The Hosmer-Lemeshow test revealed no significant deviation between nomogram predictions and outcomes (χ2 = 10.883; P = 0.207). DCA revealed that the model was clinically useful at threshold probabilities > 4%. Conclusions We developed and internally validated a nomogram predicting LVRR for pediatric DCM patients, exhibiting high sensitivity, specificity and clinical utility.
Correction of metal artefacts around orthodontic mini-implants – a micro-CT study in the rat tail model
Abstract Micro-CT enables volumetric analysis of peri-implant tissue, but grey value alterations due to metal artefacts can impair analyses. This study aimed to assess to which extent peri-implant grey values are affected by metal artefacts at increasing distance to the implant, and whether mathematical correction is possible. In nine rats, two Ti6Al4V orthodontic mini-implants (OMIs), 0.8 mm in diameter and 3.0 mm in length, were placed in a single tail vertebra. Micro-CT scans were performed before (T0) and after (T1) careful removal of the OMIs. Consecutive micro-CT scans were registered and differences in local grey values were computed at increasing distance to the implant (10.4 μm to 405.6 μm). Correction coefficient (CC) computation was performed using a smoothing spline fit, with the distance to the implant and the grey value difference as independent and dependent variable, respectively. To validate the effectiveness of the CC, the amount of calcified bone volume per total volume (BV/TV) was assessed within a standardized volume of interest (VOI) reaching up to 1 mm around the OMIs before and after the application of CC, and the T1-T0 differences between corrected and uncorrected scans were compared using the Wilcoxon signed-rank test. The grey value difference between uncorrected T0 and T1 scans was low in proximity to the implant (32.7%±6.11%) and improved at a distance of at least 100 μm (93.4%±4.46%). CC computation revealed a satisfactory fit (R2 = 0.989, RMSE = 0.031) and the difference in grey values was significantly lower after correction (p < 0.001). Most VOIs showed significant improvement, though overcorrection was observed in a few cases. Within the limitations of the study, metal artefacts decreased with increasing distance to the OMIs, and significant improvement was possible using the CC.