Browse Articles

Author Correction: A combined adjuvant and ferritin nanocage based mucosal vaccine against Streptococcus pneumoniae induces protective immune responses in a murine model

Correction: Stratum-specific health outcome estimation in Pakistan using double goal CART

Regulatory effect of agriphotovoltaic systems with different panel heights on the thermal environment

Analysis of allohexaploid wheatgrass genome reveals its Y haplome origin in Triticeae and high-altitude adaptation

Determinants of stroke among adult hypertension patients in dessie comprehensive specialized hospital and Kombolcha general hospital, Amhara, Ethiopia: A case-control study

Introduction Despite the increased admission of stroke patients in hospitals as a result of hypertension, especially in Ethiopia, there are limited studies that aim to explore important determinants of stroke, mainly among adult hypertensive patients. Therefore, this study was conducted to identify determinants of stroke among adult hypertension patients in Dessie Comprehensive Specialized Hospital and Kombolcha General Hospital, Amhara, Ethiopia. Methods An institutional-based case-control study design was conducted at Dessie Comprehensive Specialized Hospital and Kombolcha General Hospital from April 10, 2023, to May 30, 2023, on 105 cases and 420 controls selected by using a systematic random sampling technique. Data entry and processing were done using EPI-data version 4.4.2 and exported to SPSS version 26.0 for further analysis. Bi-variable and multi-variable logistic analysis was done to identify determinants of stroke among hypertension patients. Data were interpreted by using the odds ratio with a 95% confidence level, and a P-value < 0.05 was taken as statistically significant. Results A total of 105 cases and 420 controls participated in this study with a complete response (100%). Stroke among adult hypertension patients was significantly associated with a family history of hypertension (AOR =  2.8, 95% CI: 1.8–4.3), being physically inactive (AOR =  2.13, 95% CI: 1.4–3.3), current smoking cigarettes (AOR =  3.2, 95% CI: 2.1–5.2), current alcohol drinking (AOR =  4.7, 95% CI: 2.87–7.9), and having heart disease (AOR =  2.04, 95% CI: 1.3–3.25). Conclusions Having a family history of hypertension, cigarette smoking, physical inactivity, alcohol consumption, and heart disease were determinants of stroke. Health education on lifestyle practices, early hypertension, and other cardiovascular diseases in each follow-up visit is very essential for improving stroke prevention.

Exploring the relationships between macrophyte groups and environmental conditions in lake ecosystems

Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants

Correction: SYBR Gold dye enables preferential labelling of mitochondrial nucleoids and their time-lapse imaging by structured illumination microscopy

Broad-spectrum ubiquitin-specific protease inhibition as a mechanism for the cytotoxicity of YM155 in cancers

Kupffer cell and recruited macrophage heterogeneity orchestrate granuloma maturation and hepatic immunity in visceral leishmaniasis

Abstract In murine models of visceral leishmaniasis (VL), the parasitization of resident Kupffer cells (resKCs) drives early Leishmania infantum growth in the liver, leading to granuloma formation and subsequent parasite control. Using the chronic VL model, we demonstrate that polyclonal resKCs redistributed to form granulomas outside the sinusoids, creating an open sinusoidal niche that was gradually repopulated by monocyte-derived KCs (moKCs) acquiring a tissue specific, homeostatic profile. Early-stage granulomas predominantly consisted of CLEC4F + KCs. In contrast, late-stage granulomas led to remodeling of the sinusoidal network and contained monocyte-derived macrophages (momacs) along with KCs that downregulated CLEC4F, with both populations expressing iNOS and pro-inflammatory chemokines. During late-stage infection, parasites were largely confined to CLEC4F - KCs. Reduced monocyte recruitment and increased resKCs proliferation in infected Ccr2 −/− mice impaired parasite control. These findings show that the ontogenic heterogeneity of granuloma macrophages is closely linked to granuloma maturation and the development of hepatic immunity in VL.

Psychotherapies and psychological support for individuals facing psychological distress during the COVID-19 pandemic: A scoping review

In this scoping review, we investigated the psychotherapies and psychological support provided during the coronavirus disease 2019 (COVID-19) pandemic to clarify its recipients and the methods employed, among other characteristics. We used Scopus and PubMed as the two search engines and employed the following search terms: “COVID*” AND (“psychotherap*” OR “psychological support*”) AND “psychological distress*.” The first search was conducted on October 18, 2022, by reviewing search engines and conducting a manual search. It yielded 154 articles, of which 18 finally met the eligibility criteria after the second round of screening. The distribution of the participants in the intervention studies included in this review was diverse, including the general population, patients with COVID-19, and patients who had recovered from COVID-19. The implementation of psychotherapy was not limited to more advanced countries in psychiatry, indicating the broader reach of these interventions. Psychotherapy includes various methods, including cognitive behavioral therapy, acceptance commitment therapy, mindfulness, supportive care, virtual reality therapy, and online educational content via apps. The rise of new technologies may have increased the replacement rate of human therapists. In addition, the use of remote interventions was more common than that of face-to-face interventions. It is necessary to monitor whether the technologies and methods used for the first time during the pandemic will continue to be used in the future. Therefore, further research is needed to compare the effectiveness of remote randomized controlled trials with conventional face-to-face randomized controlled trials. Finally, most of those providing psychotherapies and psychological support in the studies included in this review were not doctors or psychologists.

Author Correction: Rurality and relative poverty drive acquisition of a stable and diverse gut microbiome in early childhood in a non-industrialized setting

Structure and inhibition mechanisms of Mycobacterium tuberculosis essential transporter efflux protein A

Abstract A broad chemical genetic screen in Mycobacterium tuberculosis (Mtb) identified compounds (BRD-8000.3 and BRD-9327) that inhibit the essential efflux pump EfpA. To understand the mechanisms of inhibition, we determined the structures of EfpA with these inhibitors bound at 2.7-3.4 Å resolution. Our structures reveal different mechanisms of inhibition by the two inhibitors. BRD-8000.3 binds in a tunnel contacting the lipid bilayer and extending toward the central cavity to displace the fatty acid chain of a lipid molecule bound in the apo structure, suggesting its blocking of an access route for a natural lipidic substrate. Meanwhile, BRD-9327 binds in the outer vestibule without complete blockade of the substrate path to the outside, suggesting its possible inhibition of the movement necessary for alternate access of the transporter. Our results show EfpA as a potential lipid transporter, explain the basis of the synergy of these inhibitors and their potential for combination anti-tuberculosis therapy.

Platelet rich fibrin and commercial sealants for dural closure in neurosurgery: An in vitro study

Background Watertight closure of the dura mater is essential after neurosurgical interventions to avoid complications such meningitis, intracranial hypotension and surgical site infections. In addition to conventional suturing techniques, various biomaterials have been proposed as sealants to minimize the risk of leakage. We previously reported that patient derived platelet-rich fibrin (PRF), an autologous biomaterial, significantly improves watertight dural closure when applied as an onlay over a standard running suture. To demonstrate the efficacy of this method, we aimed to compare this orthobiological approach with other commercially available sealants. Methods We utilized an in vitro testing device that allowed for the assessment of watertight dura mater closure via hydrostatic testing. On our testing phantom using freshly harvested bovine dura maters, a standardized 20-mm incision was closed using a running suture, and the leak pressure was measured initially without (primary leak pressure) and subsequently with on lay augmentation (secondary leak pressure) using either PRF, fibrinogen- and thrombin-coated collagen patch (TachoSil®), collagen matrix graft (DuraGen®), Polyethylenglykol (PEG) and polyethylene glycol ester and trilysine amine hydrogel solution (DuraSeal®), polyethylene glycol, protein-reactive polyethylene glycol monomer coated collagen matrix (Hemopatch®) or polyethylenimine component autospray sealant (Adherus®). Results The outcomes demonstrate that the usage of a dural onlay in addition to the standard running suture exhibited a substantial improvement in leak pressure compared to the running suture alone. Specifically, the median leak pressure was improved by 3.5 times, where the combined approach was able to withstand 38 cm H2O, whereas the running suture alone had a median leak pressure of 11 cm H2O. Upon evaluating the performance of the sealants, we identified two categories of dural sealants: a medium performance group (30 ± 2 cm H2O) comprised of Adherus® (31 cm H2O), DuraGen® (31 cm H2O), and Hemopatch® (28 cm H2O), and a high-performance group composed of DuraSeal® (45.5 cm H2O), and TachoSil® (41 cm H2O). Patient-derived PRF was able to withstand a max pressure of 45 cm H2O, falling into the high-performance group. Of all the sealants tested in this study, the PRF based patch demonstrated the lowest variance in leak pressure among all the tested sealants. Conclusions Autologous platelet-rich fibrin in a two-step procedure displayed enhanced augmentation and reinforced watertight closure of the dura mater, exhibiting a four-fold increase in leak pressure compared to standard running suture alone. Among other commonly utilized commercial sealants, it ranked second with demonstrated consistency in its performance.

Wavelet correlation noise analysis for qubit operation variable time series

Abstract In quantum computing, characterizing the full noise profile of qubits can aid in increasing coherence times and fidelities by developing error-mitigating techniques specific to the noise present. This characterization also supports efforts in advancing device fabrication to remove sources of noise. Qubit properties can be subject to non-trivial correlations in space and time, for example, spin qubits in MOS quantum dots are exposed to noise originating from the complex glassy behavior of two-level fluctuator ensembles. Engineering progress in spin qubit experiments generates large amounts of data, necessitating analysis techniques from fields experienced in managing large data sets. Fields such as astrophysics, finance, and climate science use wavelet-based methods to enhance their data analysis. Here, we propose and demonstrate wavelet-based analysis techniques to decompose signals into frequency and time components, enhancing our understanding of noise sources in qubit systems by identifying features at specific times. We apply the analysis to a state-of-the-art two-qubit experiment in a pair of SiMOS quantum dots with feedback applied to relevant operation variables. The observed correlations serve to identify common microscopic causes of noise, such as two-level fluctuators and hyperfine coupled nuclei, as well as to elucidate pathways for multi-qubit operation with more scalable feedback systems.

The future of antibiotic use in livestock

Abstract Governments worldwide have pledged to reduce antimicrobial use in the agri-food system. This study projects global livestock antibiotic use quantities through 2040 under various scenarios. This work indicates that under a business-as-usual scenario, global antibiotic use could reach ~143,481 tons by 2040, representing a 29.5% increase from the 2019 baseline of ~110,777 tons. However, alternative scenarios suggest that these projections could vary by +14.2% to -56.8%, depending on changes in livestock biomass and antibiotic use intensity. A key contribution of this research is the development of the Livestock Biomass Conversion method, a novel indicator offering improved accuracy in estimating livestock biomass. The findings have important policy implications, highlighting that meaningful reductions in antibiotic use quantity can only be achieved through coordinated efforts targeting both antibiotic use intensity and livestock biomass.

Premenstrual dysphoric disorder and its co-existence with depression, anxiety, and stress as risk factors for suicidal ideation and suicide attempts among university students in Bangladesh: A single-site survey

Background Premenstrual Syndrome (PMS) and Premenstrual Dysphoric Disorder (PMDD) have been identified as potential risk factors for various mental health issues, such as suicidal ideation and attempts. However, few studies have examined this association among Bangladeshi university students. This study aimed to examine the potential associations between PMDD and its co-existence with depression, anxiety, stress, and various suicidal behaviors, including suicidal ideation and attempts. Methods A cross-sectional survey involving 516 university students was conducted between September and October 2023. The survey was carried out in person and employed a structured questionnaire that comprised demographic information; the Depression, Anxiety, and Stress Scale (DASS-21); and the Premenstrual Symptoms Screening Tool (PSST), a 19-item screening tool for premenstrual symptoms. Multivariable logistic regression analyses were conducted to examine the relationships between variables. Results In the present study, participants with PMDD reported a prevalence of 38.8% for past-year suicidal ideation and 28.6% for suicide attempts. Through logistic regression analysis, we found a significant association between moderate/severe PMS and PMDD and a higher likelihoods of reporting suicidal ideation (AOR =  4.73; 95% CI 2.08–10.73 and AOR =  5.42; 95% CI 2.02–10.52) and suicide attempts (AOR =  3.77; 95% CI 1.36–10.50 and AOR =  4.07; 95% CI 1.22–15.56). The association between suicidal behaviors and PMS/PMDD was mediated by co-existing conditions such as depression, anxiety, and stress. Conclusions A notable proportion of individuals diagnosed with PMDD reported experiencing suicidal ideation or engaging in suicide attempts at some point in their lives. The findings of this research support the importance of conducting regular assessments of suicidal risk among women experiencing moderate to severe premenstrual disturbance. Furthermore, it is crucial to integrate mental health screenings and implement psychosocial interventions specifically designed for women diagnosed with PMS or PMDD and those with co-existing depression, anxiety, and stress alongside PMS/PMDD.

Bodily illusion enhances subjective fear of safety-margin violations surrounding the bodily self

Abstract All living organisms, including humans, must flexibly adjust their defensive behaviors according to the spatial proximity of potential threats. Previous research has examined fear responses to proximal threats by manipulating distance to the body in simulated 2D or 3D environments; however, the functional role of the sense of bodily self in the interplay between fear and threat proximity remains unclear. Here, we investigated fear responses to proximal and distal fear-relevant stimuli while manipulating the sense of body ownership over a mannequin using a perceptual full-body illusion. Our findings revealed that subjective fear was more sensitive to stimulus location when participants experienced illusory ownership over the mannequin. Furthermore, we observed a significant positive correlation between illusion strength and subjective fear ratings. These findings highlight two aspects of how the sense of bodily self influences human fear experiences: enhancing sensitivity to margin-of-safety violations and amplifying anxiety in response to threat appearance.

Mineralogical controls on PFAS and anthropogenic anions in subsurface soils and aquifers

Abstract Per- and polyfluoroalkyl substances (PFAS) migrate into the environment through various means, e.g., soil-amendment impurities and ambient atmospheric deposition, potentially resulting in vegetative uptake and migration to groundwater. Existing approaches for modeling sorption of PFAS commonly treat soil as an undifferentiated homogeneous medium, with distribution constants (e.g., Kd, Koc) generated empirically using surface soils. Considering the limited mineral variety expected in weathered geologic media, PFAS mobility can be better understood by accounting for predictable mineral assemblages that are ubiquitously distributed in US soils. Here we explore the role of minerals and electrostatic sorption in controlling PFAS mobility in subsurface settings at contaminated agricultural sites by measuring geochemical parameters and PFAS, and calculating pH-dependent mineral surface charges through full soil and aquifer columns. These data suggest subsurface mobility of short-chain PFAS largely is controlled by aluminum-oxide mineral(oid) electrostatic sorption, whereas long-chain PFAS mobility is controlled by organic matter and air-water interfacial area.

Comparative genomics of Mycobacterium avium subsp. hominissuis strains within a group of captive lowland tapirs

Within a group of three captive lowland tapirs (Tapirus terrestris) suffering from clinically apparent mycobacteriosis, non-tuberculous Mycobacterium avium subsp. hominissuis (MAH) strains were isolated from the animals and the tapir’s enclosure. Based on MIRU-VNTR findings, which identified two closely related INMV profiles (124 and 246), a micro-evolutionary event was assumed, and four available MAH strains were submitted to whole genome sequencing (short- and long-read technologies). Surprisingly, the differences based on single nucleotide polymorphisms (SNPs) were exceptionally high between the four strains, i.e., between 841 and 11,166 bases, due to a strong impact of homologous recombination. Thus, an ad hoc core genome multilocus sequence typing (cgMLST) scheme was created and pangenome analysis was conducted for determining the genomic similarity between the strains. The INMV246 isolate obtained from sputum on the enclosure floor and one INMV124 isolate of tapir #2 showed the highest congruence, suggesting that both originated from a shared source. The other two INMV124 isolates were genomically distinct from these strains. Nevertheless, in all four strains two plasmids were detected, which were highly conserved between the strains. The study showed that the genomic variability between MAH strains isolated from the same site within a short period of time can be exceptionally high and the influence of homologous recombination needs to be considered when determining MAH strain relationships, particularly via SNP analyses.