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Author Correction: Effectiveness of physical activity interventions on reducing perceived fatigue among adults with chronic conditions: a systematic review and meta-analysis of randomised controlled trials
Development and validation of a machine learning-based prediction model for hepatorenal syndrome in liver cirrhosis patients using MIMIC-IV and eICU databases
SLC1A5 is a key regulator of glutamine metabolism and a prognostic marker for aggressive luminal breast cancer
Abstract Cancer cells exhibit altered metabolism, often relying on glutamine (Gln) for growth. Breast cancer (BC) is a heterogeneous disease with varying clinical outcomes. We investigated the role of the amino acid transporter SLC1A5 (ASCT2) and its association with BC subtypes and patient outcomes. In large BC cohorts, SLC1A5 mRNA (n = 9488) and SLC1A5 protein (n = 1274) levels were assessed and correlated their expression with clinicopathological features, molecular subtypes, and patient outcomes. In vitro SLC1A5 knockdown and inhibition studies in luminal BC cell lines (ZR-75-1 and HCC1500) were used to further explore the role of SLC1A5 in Gln metabolism. Statistical analysis was performed using chi-squared tests, ANOVA, Spearman’s correlation, Kaplan–Meier analysis, and Cox regression. SLC1A5 mRNA and SLC1A5 protein expression were strongly correlated in luminal B, HER2 + and triple-negative BC (TNBC). Both high SLC1A5 mRNA and SLC1A5 protein expression were associated with larger tumour size, higher grade, and positive axillary lymph node metastases (P < 0.01). Importantly, high SLC1A5 expression correlated with poor BC-specific survival specifically in the highly proliferative luminal subtype (P < 0.001). Furthermore, SLC1A5 knockdown by siRNA or GPNA inhibition significantly reduced cell proliferation and glutamine uptake in ZR-75-1 cells. Our findings suggest SLC1A5 plays a key role in the aggressive luminal BC subtype and represents a potential therapeutic target. Further research is needed to explore SLC1A5 function in luminal BC and its association with Gln metabolism pathways.
Identification of hub biomarkers and immune cell infiltrations participating in the pathogenesis of endometriosis
Evaluation of feasibility accuracy and safety after 79 O-ARM based stereotactic brain biopsies
Association of the triglyceride glucose index with all cause and CVD mortality in the adults with diabetes aged < 65 years without cardiovascular disease
Integrated analysis of genetic, proteinic, and metabolomic alterations in Behcet’s disease
Investigation of genes expression of the JAK/STAT signalling pathway and AMPs in the presence of Borrelia spirochetes in Ixodes ricinus
Abstract Multicellular animals need to control the spread of invading pathogens. This is a particular challenge for blood-feeding vectors such as ticks, which ingest large amounts of blood potentially laden with harmful microorganisms. Ticks have a basic innate immune system and protect themselves from infection through innate immune responses involving pathways such as Janus kinase (JAK) or the signalling transducer activator of transcription (STAT). Direct antimicrobial defence occurs through the rapid synthesis of numerous antimicrobial agents including antimicrobial peptides (AMPs). The tick Ixodes ricinus is one of the main vectors of the Lyme disease pathogen, the spirochete Borrelia burgdorferi sensu lato. Data suggest that the JAK/STAT signalling pathway controls the expression of AMPs and regulates the infection of the pathogen in the tick body. The innate immune system during the off-host period keeps the level of spirochete infection in check. Spirochetes may influence the innate immune response in ticks. Therefore, the aim of this study was to analyse the expression of the genes related to the JAK/STAT pathway and selected AMPs in questing ticks in which B. burgorferi s.l. was detected. In the ticks infected with spirochetes, overexpression of genes related to the JAK/STAT signalling pathway was observed in the case of STAM and SOCS genes. AMPs genes such as def1, ric, lzs were overexpressed with different expression patterns. The results obtained suggest that AMPs may be involved in infection management in ticks.
Mechanistic insights into pachymic acid’s action on triple-negative breast Cancer through TOP2A targeting
Modified titanium post with polished sidewalls and prefabricated shoulders can effectively preserve the teeth with subgingival defects
Understanding the influence of stratification for mine water management: a comparative study
The expanded theory of planned behavior for energy saving among academics in Romania, Bulgaria, Turkey, and Slovakia
Abstract Given the escalating global energy consumption and the concurrent economic and energy crises, energy-saving behaviour must be adopted on a large scale. Universities that are energy-intensive institutions should be one of the institutions where energy-saving behaviour is widely adopted. Academics devote a substantial portion of their time to their offices, which leads to increased energy usage. However, no study has investigated academics’ energy-saving behaviours in the literature. Most studies focus on students or employees in various organizations. Our study tries to cover the gap by examining the energy-saving behaviour of academics in four countries (Romania, Bulgaria, Turkey, and Slovakia) based on the expanded Theory of Planned Behaviour. A questionnaire was distributed to 228 academics from the four countries to gather data. The research hypotheses were tested using partial least squares structural equation modelling. The findings show that individual factors (attitude and perceived behaviour control) influence the energy-saving intention of academics but not the organisational factors due to the weak identification with their universities. The study offers valuable insights for policymakers seeking to promote energy-saving programs in academic institutions. The academics can be seen as role models for their students which emphasizes the need to study more their sustainable behaviours.
Protective effect of low-dose lactulose in dextran sulfate sodium induced ulcerative colitis model of rats
Blue photoluminescence from active carboxyl adatoms on nanoporous anodic alumina films
Novel application of sinh cosh optimizer for robust controller design in hybrid photovoltaic-thermal power systems
A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans
A generative adversarial network with multiscale and attention mechanisms for underwater image enhancement
Evaluation and comparison of reading man flap based on different designs of angles and central axial lengths using finite element method
Field switching of microfabricated metamagnetic FeRh MRI contrast agents
Abstract In a step towards generating switchable MRI cellular labels, we demonstrate in-situ field switching of micron scale metamagnetic Iron-Rhodium (FeRh) thin film particles. A thin-film (200 nm) FeRh sample was fabricated and patterned into an array of progressively smaller squares with sizes ranging from 500 μm down to 1 μm. The large first order phase change from antiferromagnetic to ferromagnetic state was characterized using vibrating sample magnetometry, magnetic force microscopy, and MRI. Room temperature MRI experiments sensitive to the local magnetic field surrounding the particles demonstrated the low moment state (OFF MRI contrast) at 4.7T and high moment state (ON MRI contrast) at 11.7T for the array where sizes down to 2–3 μm were observed in MRI at 50 μm resolution. The expected temperature dependent MRI contrast change was seen at 4.7T, where 10 μm particles could be observed at 150 μm resolution in the ON state. A shielded MRI insert, used to temporarily increase or decrease the magnetic field up to 0.77T amplitude, was used to reversibly switch the particle array at constant temperature and blink the particles ON and OFF at 4.7T. This work demonstrates the MRI contrast switching potential for FeRh particles with biological cell dimensions, and the use of magnetic field pulses for reversible MRI label contrast control.