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Multilevel support-assisted prototype optimization network for few-shot medical segmentation of lung lesions
Exploring the expression of DLL3 in gastroenteropancreatic neuroendocrine neoplasms and its potential diagnostic value
An approach to energy conservation in lighting systems using luminaire-based sensor for automatic dimming
Synchrotron XRF spectroscopy for reading salt-encrusted cuneiform tablets
Association between ABCB4 variants and intrahepatic cholestasis of pregnancy
Preparation of curcumin submicron particles by supercritical antisolvent method with external adjustable annular gap nozzle
Novel transcripts of EMT driving the malignant transformation of oral submucous fibrosis
Abstract Oral submucous fibrosis (OSF) is a chronic, progressive, and fibrotic condition of the oral mucosa that carries an elevated risk of malignant transformation. We aimed to identify and validate novel genes associated with the regulation of epithelial-to-mesenchymal transition (EMT) in OSF. Genes regulating EMT were identified through differential gene expression analysis, using a LogFC threshold of -1 and + 1 and a padj value < 0.05, based on data from GEO datasets and the TCGA-HNSC datasets. The curated EMT genes were correlated with functional cancer states and subjected to clustering to identify candidate genes. Integration of bioinformatics and proteomics led to the discovery of the EMT genes MMP9, SPARC, and ITGA5 as novel candidates. Comprehensive pathway and immunohistochemical analyses confirmed their roles in regulating EMT in OSF, oral squamous cell carcinoma (OSCC), and OSF-associated squamous cell carcinoma (OSFSCC). The significant roles of MMP9, SPARC, and ITGA5 in fibrosis and malignancy suggest a novel mechanism in which fibrosis-associated type 2 EMT undergoes transition to type 3 EMT, driving OSF towards malignancy.