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Allogeneic iPSC-derived iNKT cells in recurrent head and neck cancer: a phase 1 trial
Sodium taurocholate promotes liver regeneration after portal vein ligation in rats
Editing the kinesin-12 gene affects responses to Bt toxin Cry1Ac in Helicoverpa zea
Abstract Crops genetically engineered to produce insecticidal proteins from Bacillus thuringiensis (Bt) are used globally to manage key insect pests. However, the evolution of resistance to Bt proteins in at least 11 pest species has reduced the effectiveness of Bt crops. Resistance to crystalline (Cry) Bt proteins including Cry1Ac produced by Bt cotton is a major problem in Helicoverpa zea (also known as bollworm and corn earworm), one of the most economically damaging pests in the United States. A previous genome-wide association study identified a nonsense point mutation in a kinesin-12 gene that was associated with resistance to Cry1Ac in a lab-selected strain of H. zea . Here, we used CRISPR/Cas9 gene editing to knock out the kinesin-12 gene in a Cry1Ac-susceptible laboratory strain, which caused a 4.0-fold increase in resistance to Cry1Ac. Conversely, gene editing that repaired the natural kinesin-12 nonsense mutation in a lab-selected resistant strain increased susceptibility to Cry1Ac by 3.8-fold. These complementary results provide compelling evidence that kinesin-12 plays a role in the mode of action of Cry1Ac against H. zea .
Meta-encryptor with multi-dimensional security architecture for wireless communications
Magnetic resonance imaging thresholds and diagnostic accuracy in unilateral congenital superior oblique muscle palsy using the ESA method
Correction: A pilot study on the effect of severe sleep fragmentation on aorta remodelling, neuroinflammation in a rat model
Double whammy: drugs that inhibit kinase enzymes also speed up their disposal
Mesh-like structure integrated core-shell-shell nanocomposites for enhanced stability and performance in carbon capture
Low neutrophil-to-lymphocyte ratio identifies secondary polycythemia in patients with erythrocytosis
Hydrology drives phytoplankton succession and productivity coupling in plateau hydropower reservoirs with implications for ecosystem health management
A large, long-lived, slowly-expanding superbubble across the Perseus arm
Optimizing sustainable hub depot locations for empty container logistics in Slovenia using particle swarm optimization
AOH induces oxidative stress and DNA damage in ovarian cancer cells via modulation of GPER1 and HIF1α/PI3K/CLDNs signaling pathway
Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity
Abstract The conserved stem domain of influenza hemagglutinin (HA), which is classified into group 1 and group 2, is a target of broadly neutralizing antibodies. While many group 1 HA stem antibodies have been described, much less is known about group 2 HA stem antibodies. This study structurally characterizes two group 2 HA stem antibodies, 2F02 and AG2-G02, targeting the central stem epitope and the lower stem epitope, respectively. Unlike prototypic antibodies to group 2 HA stem, 2F02 and AG2-G02 do not compete for binding. Both antibodies offer protection in a female mouse model via neutralization activity and Fc-mediated effector functions. We further demonstrate that the natural evolution of HA2 position 32 restricts the binding of AG2-G02 to recent human H3N2 HAs and influences the binding of human plasma samples. Overall, these findings advance our understanding of the antigenicity of HA stem, which has important implications for the development of broadly protective influenza vaccines.
Analysis of Cattaneo-Christov model in stratified and magnetized Powell-Eyring fluid flow with Joule heating and viscous dissipation
Musculoskeletal disorders among office workers: prevalence, ergonomic risk factors, and their interrelationships
Abstract This cross-sectional study investigated the prevalence of work-related musculoskeletal disorders (WMSDs), ergonomic risks, and psychosocial factors among 99 office workers at an industrial company. Participants, aged 20–50 years with minimum one year of experience, were selected using census sampling. Exclusion criteria comprised history of musculoskeletal disorders, fractures, hand surgery, major systemic diseases, pregnancy, menstruation, or recent hospitalization. Data collection utilized the extended nordic musculoskeletal questionnaire and rapid office strain assessment (ROSA). Results demonstrated 80.81% WMSDs prevalence, most commonly affecting neck (58.6%), lower back (52.5%), and shoulders (37.4%). The mean ROSA score of 5.40 ± 1.27 indicated suboptimal workstation ergonomics. Significant associations were identified between chair height and knee/shoulder/upper back pain ( p < 0.01), and between job stress and WMSDs across all body regions ( p < 0.05). The findings emphasize the necessity of comprehensive interventions targeting ergonomic improvements, postural correction, and stress management to mitigate WMSDs risks in office environments.
A distinctive human genetic lineage persisted in central Argentina for 8,500 years
Measuring location and properties of very high frequency sources emitted from an aircraft flying through high clouds
Elucidation of puberulic acid–induced nephrotoxicity using stem cell-based kidney organoids
Abstract Recent cases of acute kidney injury (AKI) in Japan have been linked to Beni-koji CholesteHelp supplements, with puberulic acid identified as a potential nephrotoxic contaminant. To address the need for a reliable in vitro nephrotoxicity testing platform, we developed a screening model using kidney organoids derived from adult rat kidney stem (KS) cells. The organoids were exposed to known nephrotoxicants, including cisplatin and gentamicin, to validate the system. Puberulic acid toxicity was evaluated in both KS cell-derived organoids and wild-type mice. The organoids recapitulated tubular injury induced by known nephrotoxins and showed significant Kim-1 mRNA upregulation. Puberulic acid-treated organoids and mice exhibited morphological features of acute tubular necrosis (ATN), mitochondrial damage, and reduced cytochrome c oxidase subunit IV (COX-IV) expression. Markers of oxidative stress and apoptosis, such as 8-hydroxy-2’-deoxyguanosine (8-OHdG) and cleaved caspase-3, were also elevated. These findings suggest that puberulic acid induces mitochondrial dysfunction and oxidative stress, leading to tubular cell death. Puberulic acid-induced nephrotoxicity was demonstrated using our kidney organoid model. KS cell-derived kidney organoids may provide a simple, reproducible, and rapid platform for nephrotoxicity assessment, which may complement conventional animal experiments.