Vertebral microstructure marks the emergence of pelagic ichthyosaurs soon after the End Permian Mass Extinction

Abstract

Abstract Ichthyosaurs were the first fully marine tetrapods, and evolved a streamlined body, flippers, live birth, and endothermy-like physiology. However, the transition to these adaptations and how it relates to divergence into ocean environments is ambiguous. Here, we use vertebral bone microstructure to document the first ontogenetic series of two Early Triassic taxa that include the oldest ichthyosaur foetal fossils. One series is from Grippia, an early ichthyopterygian with a small body, and limbs with some plesiomorphic features. The other is a large, contemporaneous ichthyosaur, Cymbospondylus. Together, they phylogenetically bracket the ichthyopterygian-ichthyosaurian transition. Grippia has a unique microstructure with a distinctive compacted outer layer, whereas Cymbospondylus vertebrae are cancellous throughout, indicating a different ecology and swimming style. The dissimilar distribution of woven-parallel complex in the histology between the two taxa indicates that growth progressed at different speeds. We also document birth lines in ichthyosaurs for the first time. Pelagic, tail-propelled, rapid-growing ichthyosaurs were thus present less than five million years after the End Permian mass extinction, alongside more anguilliform ichthyopterygians. These data capture the ecological and evolutionary transition from reptiles with eel-like swimming to whale-like ichthyosaurs, implying a paradigm shift in ecology and physiology that paved the way for ichthyosaur radiation.