Utilizing Electron‐Sink‐Enhanced Nanoshells for Amplified Nanoplasmonic SERS‐Based In Situ Detection of Cancer Cells, Linking Signal Enhancement with Cellular Damage
Abstract
Abstract A novel method is presented for detecting cancer cells and assessing apoptosis using electron‐sink‐enhanced surface‐enhanced Raman scattering (SERS) via active electron transfer. By coating gold (Au) shells with electroactive liposome membranes (ELMs) derived from Shewanella oneidensis MR‐1, the SERS signal is enhanced through chemical mechanism (CM) enhancement driven by electron transfer. The ELMs first donate electrons to the Au shells, which, upon laser excitation, amplify the local electromagnetic field, resulting in stronger Raman signals from the attached probing molecules. Additionally, the electron flow from cancer cells into the Au shells correlates with apoptosis, producing a strong SERS signal, while normal cells exhibit weaker signals. This method enables real‐time monitoring of cancer cell apoptosis, distinguishing cancer cells from normal cells based on the enhanced Raman signal linked to electron flow. This approach marks a breakthrough in CM‐based SERS applications, offering a sensitive method for cancer detection through the measurement of electron flow.
Article Details
Authors (16)
Liu‐Chun Wang
Department of Chemistry National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China
Yu‐Cheng Kuo
Program on Smart and Sustainable Manufacturing Academy of Innovative Semiconductor and Sustainable Manufacturing National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China
Yen‐Ting Kuo
Department of Chemistry National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China
Kai‐Lin Chang
Department of Chemistry National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China
Ying‐Chi Chen
Department of Chemistry National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China
Wen‐Jyun Wang
Department of Medicinal and Applied Chemistry Kaohsiung Medical University No. 100, Shih‐Chuan 1st Road, Sanmin Dist. Kaohsiung City Taiwan 80708 China
Ming‐Yuan Hung
Program on Smart and Sustainable Manufacturing Academy of Innovative Semiconductor and Sustainable Manufacturing National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China
Fang‐Yi Hsu
Department of Medicinal and Applied Chemistry Kaohsiung Medical University No. 100, Shih‐Chuan 1st Road, Sanmin Dist. Kaohsiung City Taiwan 80708 China
Pooja Aich
Department of Medicinal and Applied Chemistry Kaohsiung Medical University No. 100, Shih‐Chuan 1st Road, Sanmin Dist. Kaohsiung City Taiwan 80708 China
Yu‐Wei Lin
Department of Materials Science and Engineering Feng Chia University No. 100, Wenhwa Road, Seatwen Taichung Taiwan 40724 China
Chia‐Hao Su
Center for General Education Chang Gung University No.259, Wenhua 1st Road, Guishan Dist., Taoyuan City Taiwan 33302 China
Divinah Manoharan
Yi‐Hsin Chien
Department of Materials Science and Engineering Feng Chia University No. 100, Wenhwa Road, Seatwen Taichung Taiwan 40724 China
Wei‐Peng Li
Department of Medicinal and Applied Chemistry Kaohsiung Medical University No. 100, Shih‐Chuan 1st Road, Sanmin Dist. Kaohsiung City Taiwan 80708 China
Hong‐Kang Tian
Program on Smart and Sustainable Manufacturing Academy of Innovative Semiconductor and Sustainable Manufacturing National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China
Chen‐Sheng Yeh
Department of Chemistry National Cheng Kung University No.1, University Road, Tainan City 701 Taiwan China