Unveiling the glymphatic system’s role in brain aging: A comprehensive biomarker and modifiable intervention target

Y Yuanyuan Fang (Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) W Wenxi Luo (College of Life Science and Technology, Huazhong University of Science and Technology) H Hao Huang L Lusen Ran (Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) Y Yuqin He (Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) C Chang Cheng (Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) Y Yao Yao Y Yuxiang Hou (School of Computer Science and Technology, National Engineering Research Center for Big Data Technology and System, Services Computing Technology and System Lab, Cluster and Grid Computing Lab, Huazhong University of Science and Technology) H Haibo Zheng (School of Computer Science and Technology, National Engineering Research Center for Big Data Technology and System, Services Computing Technology and System Lab, Cluster and Grid Computing Lab, Huazhong University of Science and Technology) D Dengji Pan (Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) S Shabei Xu (Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) X Xiang Luo (Ophthalmology Medical Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, Chongqing Branch (Municipality Division) of National Clinical Research Centre for Ocular Diseases) T Tingting Qin (Clinical Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) X Xingjie Hao (Department of Epidemiology and Biostatistics, Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology) F Feng Lu (Department of Medical Oncology, Dana-Farber Cancer Institute) W Wei Wang M Minghuan Wang (Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology)

Abstract

The focus of this study is to investigate the role of diffusion tensor imaging along the perivascular space (DTI-ALPS) index in brain aging. To address this, we first examined the association of DTI-ALPS with aging hallmarks among 40,488 UK Biobank (UKB) participants. Next, we developed normative brain age models incorporating the DTI-ALPS index from 12,401 healthy UKB adults and validated in UKB-ADNI and UKB-TALENT datasets. Finally, we explored the relationship between brain age gap (BAG) with peripheral organ function, chronic diseases, proteomics, and genetics, while identifying modifiable factors in a longitudinal cohort. The findings revealed that DTI-ALPS index correlated with chronological age, telomere length, brain structure, and cognition. A brain age model integrating the DTI-ALPS index achieved good accuracy in the UKB ( r = 0.756) and replicated well in two independent datasets (UKB-ADNI: r = 0.766; UKB-TALENT: r = 0.724), with choroid plexus volume emerging as an additional contributor. Musculoskeletal health was a key driver for brain aging in females, while pulmonary metrics prevailed in males. Neurodegenerative and metabolic disorders increased BAGs, imparting increased mortality risk. Protein-wide and genome-wide analysis identified 154 BAG-related proteins and 11 loci. Modifiable factors, particularly systolic blood pressure below 120 mmHg, were strongly associated with reduced BAGs. Overall, the DTI-ALPS index is a promising brain aging biomarker, offering insights into links between brain and peripheral health, and highlighting sex-stratified therapeutic strategies. Aggressive blood pressure control may mitigate brain aging and promote long-term brain health.

Article Details

Volume / Issue Vol. 123, Issue 18
Published May 05, 2026
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (17)

Y

Yuanyuan Fang

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

W

Wenxi Luo

College of Life Science and Technology, Huazhong University of Science and Technology

H

Hao Huang

L

Lusen Ran

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

Y

Yuqin He

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

C

Chang Cheng

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

Y

Yao Yao

Y

Yuxiang Hou

School of Computer Science and Technology, National Engineering Research Center for Big Data Technology and System, Services Computing Technology and System Lab, Cluster and Grid Computing Lab, Huazhong University of Science and Technology

H

Haibo Zheng

School of Computer Science and Technology, National Engineering Research Center for Big Data Technology and System, Services Computing Technology and System Lab, Cluster and Grid Computing Lab, Huazhong University of Science and Technology

D

Dengji Pan

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

S

Shabei Xu

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

X

Xiang Luo

Ophthalmology Medical Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, Chongqing Branch (Municipality Division) of National Clinical Research Centre for Ocular Diseases

T

Tingting Qin

Clinical Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

X

Xingjie Hao

Department of Epidemiology and Biostatistics, Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology

F

Feng Lu

Department of Medical Oncology, Dana-Farber Cancer Institute

W

Wei Wang

M

Minghuan Wang

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology