Underappreciated role of canopy nitrogen deposition for forest productivity

X Xiaowei Li (College of Chemistry, Institute of Nuclear Science and Technology, Key Laboratory of Radiation Physics and Technology of Ministry of Education) C Chenlu Zhang B Beibei Zhang (State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC)) L Li Jiang (Department of Radiation Oncology The First Affiliated Hospital of Guangxi Medical University Nanning China) S Shengqi Tang (College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University) C Chenhui Sun (College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University) Y Yulong Bai (Department of Chemistry, Westlake University, 600 Dunyu Road, Hangzhou 310030, P. R. China) Y Yubang Wang (College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University) Y Yifei Shi (College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University) L Lei Ma W Wei Zhang Q Qing Ye (Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences) J Junhua Yan (Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences) K Keya Wang (Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences) J Juemin Fu (Jigongshan National Nature Reserve) W Wenzhi Du (Jigongshan National Nature Reserve) D Denglong Ha (Jigongshan National Nature Reserve) Y Yuxi Ju (Jigongshan National Nature Reserve) S Shiqiang Wan (College of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Institute of Life Science and Green Development, Hebei University) L Liang Hong (Centre for Clean Energy Technology, Faculty of Science) Y Yunting Fang (Chinese Academy of Sciences Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences) E Evan Siemann (Biosciences Department, Rice University) Y Yiqi Luo P Peter B. Reich S Shenglei Fu (College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University)

Abstract

Atmospheric nitrogen (N) deposition is generally expected to stimulate plant carbon (C) sequestration and promote tree growth, thereby mitigating atmospheric CO 2 accumulation. Yet, the magnitude of N deposition contribution to forest productivity remains contentious. While correlative studies suggest substantial plant growth enhancement, controlled fertilization experiments typically demonstrate a limited impact. This discrepancy may arise from whether or not to consider canopy N uptake processes. Here, we conducted a 10-y field experiment comparing canopy addition of N (CAN) with understory addition of N (UAN) at the rate of 0, 25, and 50 kg N ha –1 y –1 in a temperate deciduous forest in central China. We show that CAN significantly enhanced net primary productivity by 37.0% over control, driven by enhanced leaf litterfall, wood and fine root production, whereas UAN effects were marginal (8%). 15 N isotopic tracing revealed that CAN, through enhanced plant N uptake and increased ecosystem N retention, yielded a 3.5-fold higher C sequestration efficiency (∆C/∆N) of 54.5 ± 7.7 kg C kg –1 N, than UAN (12.2 ± 3.4 kg C kg –1 N) resulted from greater N loss through leaching. Physiological measurements indicated CAN enhanced leaf photosynthetic rates, modified leaf morphology, and extended leaf lifespan via delayed senescence. These findings provide robust empirical evidence that canopy N uptake is crucial for maximizing N-induced forest productivity, thereby holding significant implications for refining global C models and urging modelers to incorporate canopy processes for more accurate projections of future C sinks and climate change mitigation strategies.

Article Details

Volume / Issue Vol. 122, Issue 34
Published August 26, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (25)

X

Xiaowei Li

College of Chemistry, Institute of Nuclear Science and Technology, Key Laboratory of Radiation Physics and Technology of Ministry of Education

C

Chenlu Zhang

B

Beibei Zhang

State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC)

L

Li Jiang

Department of Radiation Oncology The First Affiliated Hospital of Guangxi Medical University Nanning China

S

Shengqi Tang

College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University

C

Chenhui Sun

College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University

Y

Yulong Bai

Department of Chemistry, Westlake University, 600 Dunyu Road, Hangzhou 310030, P. R. China

Y

Yubang Wang

College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University

Y

Yifei Shi

College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University

L

Lei Ma

W

Wei Zhang

Q

Qing Ye

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences

J

Junhua Yan

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences

K

Keya Wang

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences

J

Juemin Fu

Jigongshan National Nature Reserve

W

Wenzhi Du

Jigongshan National Nature Reserve

D

Denglong Ha

Jigongshan National Nature Reserve

Y

Yuxi Ju

Jigongshan National Nature Reserve

S

Shiqiang Wan

College of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Institute of Life Science and Green Development, Hebei University

L

Liang Hong

Centre for Clean Energy Technology, Faculty of Science

Y

Yunting Fang

Chinese Academy of Sciences Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences

E

Evan Siemann

Biosciences Department, Rice University

Y

Yiqi Luo

P

Peter B. Reich

S

Shenglei Fu

College of Geographical Science, Faculty of Geographical Science and Engineering, Henan University