The LFR-SWI/SNF complex: A chromatin wrench safeguarding cellular quiescence in the <i>Arabidopsis</i> root stem cell organizer
Abstract
The quiescent center (QC) resides in a reversible G 0 state in which cells are not actively dividing and yet retain their proliferation competence upon stimulation. How this quiescent state is molecularly defined and stably maintained is a fascinating question. Here, we uncover a dual role for LEAF AND FLOWER RELATED (LFR), a component of the SWITCH/SUCROSE NONFERMENTABLE (SWI/SNF) chromatin-remodeling complex, in maintaining quiescence of the QC. We demonstrate that LFR is recruited to the chromatin of core transcription factors (TFs) PLETHORA 1 (PLT1), PLT2, SCARECROW (SCR), and WUSCHEL - RELATED HOMEOBOX (WOX5) via physical interactions with them. Moreover, the autoregulatory binding of these TFs reciprocally requires LFR. Functioning as a chromatin wrench, LFR relaxes the chromatin at PLT1 , PLT2 , and SCR loci to sustain their positive autoregulation, thereby contributing to the prevention of QC cell division. Conversely, LFR compacts WOX5 chromatin to enforce negative autoregulation and simultaneously promotes CYCD3;3 expression to counteract WOX5-mediated repression, thus ensuring the proliferation competence of the QC. Furthermore, WOX5 throws a wrench into LFR binding at the CYCD3;3 promoter, establishing a regulatory circuit that precisely modulates CYCD3;3 expression, a D-type cyclin whose appropriate level is critical for QC quiescence. Consequently, the QC is maintained in a proliferation-competent but arrested state. Our findings establish the LFR-containing SWI/SNF complex as a key regulatory node that coordinates TF autoregulation with cell-cycle control to maintain QC quiescence.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (14)
Shuge Wang
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University
Jiayu Li
Mengmeng Li
Qiyan Fang
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University
Xiaolei Shi
Hebei Laboratory of Crop Genetics and Breeding, National Soybean Improvement Center Shijiazhuang Sub-Center, Ministry of Agriculture and Rural Affairs, Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences
Long Yan
Qiang Chen
Xiaoyun Li
Department of Chemistry
Muhammad Ans Aslam
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University
Hao Zhang
Xigang Liu
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University
Wenqiang Tang
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University
Sujuan Cui
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University
Hongtao Zhao
Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University