The Japanese Archipelago sheltered cave lions, not tigers, during the Late Pleistocene

X Xin Sun L Lanhui Peng (The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University) T Takumi Tsutaya (Center for GeoGenetics, The Globe Institute, University of Copenhagen) Q Qigao Jiangzuo (Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences) Y Yoshikazu Hasegawa (Iida City Museum) Y Yuxin Hou (The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University) Y Yu Han Y Yan Zhuang (The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University) N Nuno Filipe Gomes Martins (Center for Evolutionary Hologenomics, The Globe Institute, University of Copenhagen) J Jazmin Ramos Madrigal (Center for Evolutionary Hologenomics, The Globe Institute, University of Copenhagen) A Alberto J. Taurozzi (Center for GeoGenetics, The Globe Institute, University of Copenhagen) M Meaghan Mackie G Gaudry Trochė (Center for GeoGenetics, The Globe Institute, University of Copenhagen) J Jesper V. Olsen E Enrico Cappellini S Stephen J. O’Brien (Guy Harvey Oceanographic Center, Halmos College of Arts and Sciences, Nova Southeastern University) M M. Thomas P. Gilbert N Nobuyuki Yamaguchi (Wildlife Conservation Research Unit, Department of Biology, University of Oxford) S Shu-Jin Luo (The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University)

Abstract

Lions and tigers, as dominant apex predators, likely became competitors when lions expanded from Africa into Eurasia approximately one million years ago (Ma), forming a lion–tiger transition belt from the Middle East through Central Asia to the Russian Far East. At the easternmost edge of this zone, the Japanese Archipelago has long been considered a Late Pleistocene tiger refugium, supported by large felid subfossils traditionally attributed to tigers ( Panthera tigris ), though their taxonomic identity remained unresolved. To clarify the origin, evolutionary history, and biogeography of Japan’s Pleistocene felids, we analyzed 26 ancient specimens previously assumed to be tigers. Using mitochondrial and nuclear genome hybridization capture and sequencing, paleoproteomics, Bayesian molecular dating, and radiocarbon dating, we found that all ancient Japanese “tiger” remains yielding molecular data were, unexpectedly, cave lions ( Panthera spelaea ). One specimen from Yamaguchi Prefecture, western Japan, was radiocarbon dated to 36,000-34,891 cal. BP. These cave lions likely dispersed to the Japanese Archipelago between ~72.7 and 37.5 thousand years ago (ka), when a land bridge connected northern Japan to the mainland during the Last Glacial Period. Our findings challenge the long-held view that tigers once took refuge in Japan, showing instead that cave lions were widespread in northeast Asia during this period and were the Panthera lineage that colonized Japan, reaching even its southwestern regions despite habitats previously thought to favor tigers.

Article Details

Volume / Issue Vol. 123, Issue 6
Published February 10, 2026
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (19)

X

Xin Sun

L

Lanhui Peng

The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University

T

Takumi Tsutaya

Center for GeoGenetics, The Globe Institute, University of Copenhagen

Q

Qigao Jiangzuo

Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences

Y

Yoshikazu Hasegawa

Iida City Museum

Y

Yuxin Hou

The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University

Y

Yu Han

Y

Yan Zhuang

The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University

N

Nuno Filipe Gomes Martins

Center for Evolutionary Hologenomics, The Globe Institute, University of Copenhagen

J

Jazmin Ramos Madrigal

Center for Evolutionary Hologenomics, The Globe Institute, University of Copenhagen

A

Alberto J. Taurozzi

Center for GeoGenetics, The Globe Institute, University of Copenhagen

M

Meaghan Mackie

G

Gaudry Trochė

Center for GeoGenetics, The Globe Institute, University of Copenhagen

J

Jesper V. Olsen

E

Enrico Cappellini

S

Stephen J. O’Brien

Guy Harvey Oceanographic Center, Halmos College of Arts and Sciences, Nova Southeastern University

M

M. Thomas P. Gilbert

N

Nobuyuki Yamaguchi

Wildlife Conservation Research Unit, Department of Biology, University of Oxford

S

Shu-Jin Luo

The State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Institute of Ecology, Peking University