Temporal and spatial coordination of DNA segregation and cell division in an archaeon
Abstract
Cells must coordinate DNA segregation with cytokinesis to ensure that each daughter cell inherits a complete genome. Here, we explore how DNA segregation and division are mechanistically coupled in archaeal relatives of eukaryotes, which lack Cyclin-dependent kinase (CDK)/Cyclins. Using live cell imaging, we first describe the series of sequential changes in DNA organization that accompany cell division in Sulfolobus, which computational modeling shows likely aid genome segregation. Through a perturbation analysis we identify a regulatory checkpoint which ensures that the compaction of the genome into two spatially segregated nucleoids only occurs once cells have assembled a division ring—which also defines the axis of DNA segregation. Finally, we show that DNA compaction and segregation depend, in part, on a ParA homologue, SegA, and its partner SegB, whose absence leads to bridging DNA. Taken together, these data show how regulatory checkpoints like those operating in eukaryotes aid high-fidelity division in an archaeon.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (13)
Joe Parham
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Valerio Sorichetti
Institute of Science and Technology Austria
Alice Cezanne
Sherman Foo
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Yin-Wei Kuo
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Baukje Hoogenberg
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Arthur Radoux-Mergault
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Eloise Mawdesley
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Lydia Daniels Gatward
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Jerome Boulanger
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Ulrike Schulze
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology
Anđela Šarić
Institute of Science and Technology Austria
Buzz Baum
Division of Cell Biology, Medical Research Council Laboratory of Molecular Biology