Targeting the polyene chain represents an adjuvant strategy for optimizing polyene antifungals

Y Yishan Zhang (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) X Xiaocong Liu (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) L Luobei Weng (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) S Shuixiu Li (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) P Peiran Chen (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) G Gehua Zhang (Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou) J Jianqi Wang (Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou) D Dongmei Zhang (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) C Chuanyan Tang (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) Y Yunfeng Hu (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) W Wencai Ye (State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou) H Hong Zhang

Abstract

Polyene antifungals (PAs) have long constituted an irreplaceable lifesaving treatment for lethal fungal infections. However, PAs induce significant dose-dependent nephrotoxicity, primarily because of their binding to cholesterol in the renal cell membrane, which is structurally similar to their fungicidal target ergosterol in the fungal membrane. Efforts to optimize PAs have focused mainly on their mycosamine group and polyol chain, while the potential of the polyene chain remains underexplored. Here, we report that the polyene chain can function as a molecular switch, enabling hydrophobic coupling with structurally compatible adjuvants repurposed from clinically approved drugs. This hydrophobic coupling triggers conformational rearrangements of PAs, which are characterized by a reorientation of the mycosamine group, and increases the rigidity and length of the polyene chain, which can asymmetrically modulate the binding of PAs to membrane cholesterol and ergosterol, thereby attenuating nephrotoxicity and increasing effectiveness. Among them, vitamin D 3 demonstrated immediate clinical value, characterized by its broad safety profile and substantial dual adjuvant activities in multiple animal models and retrospective clinical data. Thus, exploiting the polyene chain as a molecular switch, modulated via specific polyene chain–coupled adjuvants, is a strategy to concurrently attenuate nephrotoxicity and potentiate the effectiveness of PAs.

Article Details

Volume / Issue Vol. 123, Issue 17
Published April 28, 2026
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (12)

Y

Yishan Zhang

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

X

Xiaocong Liu

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

L

Luobei Weng

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

S

Shuixiu Li

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

P

Peiran Chen

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

G

Gehua Zhang

Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou

J

Jianqi Wang

Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou

D

Dongmei Zhang

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

C

Chuanyan Tang

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

Y

Yunfeng Hu

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

W

Wencai Ye

State Key Laboratory of Bioactive Molecules and Druggability Assessment, The First Affiliated Hospital of Jinan University Guangzhou

H

Hong Zhang