Superfast Protein Desulfurization Triggered by Low‐Energy Visible Light
Abstract
Abstract The combination of transthioesterification‐based ligation of thiol‐derived amino acids and the post‐ligation desulfurization has greatly expanded the scope of modern chemical protein synthesis. Here, we report a new strategy of low‐energy visible light‐induced desulfurization (LEnVLD) that enables superfast and clean protein desulfurization (half life = 1.7 s) with improved reaction selectivity compared to the previous methods. The LEnVLD method can be easily carried out under very mild conditions using only a catalytic amount of fluorescent dye and household flashlight (ca. 5 W) irradiation, eliminating the need for any pyrophoric reagent, thiol additives, or excessive radical initiators, and its practicality was demonstrated by the fast and high‐yielding desulfurization of more than 30 peptide and protein substrates bearing a variety of sensitive functional groups (e.g., Thz, N‐alkylated maleimide, and thioester). Moreover, the convenience and robustness of LEnVLD enable its extension to versatile reaction scenarios (e.g., solid‐supported desulfurization and flow chemistry‐based desulfurization) that would enhance the practical capability of chemical protein synthesis.
Article Details
Authors (8)
Dongyang Han
New Cornerstone Science Laboratory, Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry
Xiangyu Deng
New Cornerstone Science Laboratory, Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry
Yan Cui
Xianglai Zhu
New Cornerstone Science Laboratory, Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry
Guiyu Deng
New Cornerstone Science Laboratory Tsinghua‐Peking Joint Center for Life Sciences MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Center for Synthetic and Systems Biology Department of Chemistry Tsinghua University Beijing 100084 China
Lu‐Jun Liang
Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at Microscale University of Science and Technology of China Hefei 230026 China
Guo‐Chao Chu
Division of Life Sciences and Medicine University of Science and Technology of China Hefei 230022 China
Lei Liu