Spinal Cord‐Like Scaffold with Rapid Tissue Integration Enhanced Spinal Cord Nerve Repair
Abstract
Abstract Spinal cord injury (SCI) repair represents an immense challenge globally. Despite various beneficial strategies having been attempted for SCI repair, the ideal therapy has not yet been achieved. In this study, a multidimensional biomimetic spinal cord‐like scaffold (MBSLS) is designed with a hierarchical structure and embryonic neurogenetic microenvironment and neural regeneration in SCI repair. MBSLS successfully regulated the neural differentiation of loaded neural stem cells (NSCs) and regenerated spinal cord‐like tissue in vitro by promoting neurogenesis, gliogenesis, axonogenesis, axon ensheathment, synapse organization, and calcium homeostasis. Moreover, the NSC‐loaded scaffold achieves exceptional SCI repair through highly efficient axonal regeneration and neural circuit reconstruction, which significantly improves the recovery of motor function, sensory function, and autonomic function in vivo. Importantly, the MBSLS scaffold achieves seamless integration with the host spinal cord in only 5 min, allowing early cell infiltration and repair. Finally, the feasibility of the minimally invasive implantation of human‐sized MBSLS in a pig SCI model is confirmed, demonstrating its potential and prospects for clinical translation to treat SCI.
Article Details
Authors (20)
Baoshuai Bai
Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Advanced Medical Research Institute, Cheeloo College of Medicine Shandong University Jinan Shandong P. R. China
Ruizhi Zhang
Chi Zhang
Yanhan Liu
Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, and Department of Ophthalmology, Renji Hospital, School of Medicine
Shuo Liu
Chenbo Zou
Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Advanced Medical Research Institute, Cheeloo College of Medicine Shandong University Jinan Shandong P. R. China
Linlin Jiang
Zhangyang Qi
Department of Orthopaedics Qilu Hospital of Shandong University Shandong University Centre for Orthopaedics Advanced Medical Research Institute Cheeloo College of Medicine Shandong University Jinan 250012 P. R. China
Xingqi Song
Department of Vascular Surgery Shanghai Key Lab of Tissue Engineering Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai P. R. China
Chunlin Li
Key Laboratory of Advanced Catalysis, Gansu Province; State Key Laboratory of Natural Product Chemistry, College of Chemistry and Chemical Engineering
Zhen Li
Kailin Wu
Department of Orthopaedics Tianjin Medical University General Hospital, International Science and Technology Cooperation Base of Spinal Cord Injury Tianjin Key Laboratory of Spine and Spinal Cord Tianjin 300052 P. R. China
Yijie Song
Mengfan Hou
Department of Orthopaedics Tianjin Medical University General Hospital, International Science and Technology Cooperation Base of Spinal Cord Injury Tianjin Key Laboratory of Spine and Spinal Cord Tianjin 300052 P. R. China
Chao Li
Guangdong Zhou
Xiaohong Kong
Department of Orthopaedics Qilu Hospital of Shandong University Shandong University Centre for Orthopaedics Advanced Medical Research Institute Cheeloo College of Medicine Shandong University Jinan 250012 P. R. China
Dong Lei
Hengxing Zhou
Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Advanced Medical Research Institute, Cheeloo College of Medicine Shandong University Jinan Shandong P. R. China
Shiqing Feng