Small-molecule allosteric activator of ubiquitin-specific protease 7 (USP7)
Abstract
Ubiquitin-specific protease 7 (USP7) is a deubiquitylase essential for cell homeostasis, DNA repair, and regulation of both tumor suppressors and oncogenes. Recently, haploinsufficiency of USP7 has been associated with Hao–Fountain syndrome (HAFOUS), a rare neurodevelopmental disorder. Although a range of USP7 inhibitors have been developed over the last decade, in the context of HAFOUS, USP7 activators may represent a more relevant approach. To address this challenge, we report the identification and characterization of a small-molecule activator of USP7 called MS-8. Structural and functional studies show that MS-8 allosterically activates USP7, mimicking the endogenous autoactivation mechanism of the enzyme. We observed that MS-8 engages and activates mutant USP7 in a cellular context, impacting downstream proteins. Taken together, our study provides validation of the USP7 activator that paves the way toward activation-driven USP7 pharmacology.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (14)
Isabella Jaen Maisonet
Department of Cancer Biology, Dana-Farber Cancer Institute
Mona Sharafi
Department of Cancer Biology, Dana-Farber Cancer Institute
Emilie J. Korchak
Department of Molecular Biology and Biophysics, University of Connecticut
Andres Salazar-Chaparro
Department of Cancer Biology, Dana-Farber Cancer Institute
Ariana S. Bratt
Department of Cancer Biology, Dana-Farber Cancer Institute
Tvesha Parikh
Department of Molecular Biology and Biophysics, University of Connecticut
Anthony C. Varca
Department of Cancer Biology, Dana-Farber Cancer Institute
Binita Shah
Department of Cancer Biology, Dana-Farber Cancer Institute
Michael Darnowski
Department of Cancer Biology, Dana-Farber Cancer Institute
Mia Chung
Department of Cancer Biology, Dana-Farber Cancer Institute
Wei Pin Teh
Department of Cancer Biology, Dana-Farber Cancer Institute
Jianwei Che
Department of Cancer Biology, Dana-Farber Cancer Institute
Irina Bezsonova
Sara J. Buhrlage
Department of Cancer Biology, Dana-Farber Cancer Institute