SiR‐XActin: A Fluorescent Probe for Imaging Actin Dynamics in Live Cells
Abstract
Abstract Imaging actin‐dependent processes in live cells is important for understanding numerous biological processes. However, currently used natural‐product‐based fluorescent probes for actin filaments affect the dynamics of actin polymerization and can induce undesired cellular phenotypes. Here, we introduce SiR‐XActin, a simplified jasplakinolide‐based, far‐red fluorescent probe that enables bright and photostable staining in various cell types without requiring genetic modifications. Due to its relatively weak binding affinity, the probe exhibits minimal cytotoxicity and labels actin filaments without significantly altering actin dynamics. Furthermore, SiR‐XActin is suitable for time‐resolved, live‐cell super‐resolution STED microscopy. Exchanging the SiR fluorophore in SiR‐XActin for other fluorophores yields probes in different colors. All these properties make SiR‐XActin and its analogs powerful tools for studying actin dynamics using live‐cell fluorescence microscopy.
Article Details
Authors (15)
Veselin Nasufovic
Julian Kompa
Department of Chemical Biology
Halli L. Lindamood
Department of Neuroscience and Regenerative Medicine Medical College of Georgia at Augusta University 1120 15th Street Augusta Georgia 30912 USA
Merle Blümke
Institut für Organische und Makromolekulare Chemie Friedrich‐Schiller‐Universität Humboldtstr. 10 D‐07743 Jena Germany
Rayane Dibsy
Membrane, Cytoskeleton and Cell Division Unit CNRS UMR3691 Institut Pasteur Université Paris Cité 25–28 rue du Dr Roux Paris F‐75015 France
Birgit Koch
Victoria Levario‐Diaz
Department of Cellular Biophysics Max Planck Institute for Medical Research Jahnstrasse 29 D‐69120 Heidelberg Germany
Katharina Weber
Department of Cellular Biophysics Max Planck Institute for Medical Research Jahnstrasse 29 D‐69120 Heidelberg Germany
Marlene Maager
Department of Cellular Biophysics Max Planck Institute for Medical Research Jahnstrasse 29 D‐69120 Heidelberg Germany
Ekaterina Nomerotskaia
Institut für Organische und Makromolekulare Chemie Friedrich‐Schiller‐Universität Humboldtstr. 10 D‐07743 Jena Germany
Arnaud Echard
Elisabetta Ada Cavalcanti‐Adam
Department of Cellular Biophysics Max Planck Institute for Medical Research Jahnstrasse 29 D‐69120 Heidelberg Germany
Eric A. Vitriol
Hans‐Dieter Arndt
Institut für Organische und Makromolekulare Chemie Friedrich‐Schiller‐Universität Humboldtstr. 10 D‐07743 Jena Germany
Kai Johnsson
Department of Chemical Biology