Single-cell elderly blood–CSF atlas implicates peripherally influenced immune dysregulation in normal pressure hydrocephalus
Abstract
We have generated a single-cell RNA sequencing atlas of peripheral blood and ventricular CSF in idiopathic normal pressure hydrocephalus (iNPH) patients totaling 140,207 single-cell transcriptomes. We found proinflammatory alterations in peripheral blood and CSF monocytes in iNPH patients with lower baseline cognitive function. We also identified CSF cell populations likely representing periventricular sloughing of degenerating neuroglial cells. Our findings suggest possible immune dysregulation in the blood and CSF of iNPH patients.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (17)
Phan Q. Duy
Emre Kiziltug
Department of Neurosurgery, Massachusetts General Hospital
Ana B.W. Greenberg
Department of Neurosurgery, Massachusetts General Hospital
Neel H. Mehta
Department of Neurosurgery, Massachusetts General Hospital
Le T. Hao
Department of Neurosurgery, Yale University School of Medicine
Carla Fortes
Department of Neurosurgery, Massachusetts General Hospital
Susan Mullany
Department of Neurosurgery, Massachusetts General Hospital
Baojian Fan
Department of Neurosurgery, Massachusetts General Hospital
Ani Manichaikul
Department of Genome Sciences, Center for Public Health Genomics, University of Virginia
Andrew F. Teich
Department of Pathology and Cell Biology, Columbia University
Diane Chan
Department of Neurology, Massachusetts General Hospital
Seth L. Alper
Division of Nephrology and Department of Medicine, Beth Israel Deaconess Medical Center
Bradley T. Hyman
Steven E. Arnold
Department of Neurology, Massachusetts General Hospital
Guy M. McKhann
Department of Neurosurgery, Columbia University
Matthew P. Frosch
Department of Neurology, Massachusetts General Hospital
Kristopher T. Kahle
Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School