Serum metabolic patterns reveal the diagnostic and prognostic role of alanine abnormality in ocular adnexal lymphoma

Y Yida Huang (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) H Huimin Lin (Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine) J Jiahao Shi (Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine) Y Yifan Chen Q Qi Sang (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) R Ruimin Wang (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) W Wanshan Liu (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) J Jiao Wu (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) Y Yanyan Li (Department of Chemical Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, Guangdong, China.) X Xiaoyu Xu (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) C Chunmeng Ding (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) S Shouzhi Yang (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) J Juxiang Zhang (State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University) R Renbing Jia (Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine) X Xianqun Fan (Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine) K Kun Qian (Dongguan Key Laboratory of Interdisciplinary Science for Advanced Materials and Large-Scale Scientific Facilities, School of Physical Sciences) Y Yixiong Zhou (Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine)

Abstract

Ocular adnexal lymphoma (OAL) is the most common orbital malignancy in adults. Advanced tools for precise diagnosis and prognosis of OAL are in demand. Here, the nanoparticle-enhanced laser desorption/ionization mass spectrometry was applied for the construction of OAL-associated serum metabolic patterns (SMPs) from 239 participants (104 OAL and 135 non-OAL). Through machine learning, the diagnostic performance with an area-under-the-curve (AUC) of 0.901 was achieved for OAL based on SMPs. Furthermore, a diagnostic metabolic panel was constructed with an AUC of 0.875. Moreover, a prognosis scoring system was built and its desirable prediction efficacy for progression-free survival of OAL was confirmed ( P < 0.05). Specifically, the decreased serum alanine level was found to play both vital roles in the diagnosis and prognosis of OAL, and we demonstrated that uptake of alanine promotes glycolysis and cell growth in lymphoma cells. Our study highlights the value of the serum metabolite biomarkers in the clinical management of OAL.

Article Details

Volume / Issue Vol. 122, Issue 31
Published August 05, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (17)

Y

Yida Huang

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

H

Huimin Lin

Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine

J

Jiahao Shi

Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine

Y

Yifan Chen

Q

Qi Sang

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

R

Ruimin Wang

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

W

Wanshan Liu

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

J

Jiao Wu

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

Y

Yanyan Li

Department of Chemical Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, Guangdong, China.

X

Xiaoyu Xu

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

C

Chunmeng Ding

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

S

Shouzhi Yang

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

J

Juxiang Zhang

State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University

R

Renbing Jia

Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine

X

Xianqun Fan

Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine

K

Kun Qian

Dongguan Key Laboratory of Interdisciplinary Science for Advanced Materials and Large-Scale Scientific Facilities, School of Physical Sciences

Y

Yixiong Zhou

Department of Ophthalmology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine